SASDXZ5

SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2025-09-01T15:11:14.749430+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL

浓度— – 1.5 缓冲液 / pH20 mM Tris, 150 mM NaCl / 7.5
Experimental temperature25.0 设备 / 束线National Synchrotron Light Source II (NSLS-II) / 16-ID (LiX)
波长0.08189 nm曝光0.5 s × 10

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Nucleoprotein
查看序列
MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
proteinSevere acute respiratory syndrome coronavirus 2P0DTC92–419dimer分子数 247.3 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1101[3]0.005–0.25 1/A含误差列缺失 00sasbdb/entries/z5/sasdxz5/source/SASDXZ5.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)18.80.9Å
i0Guinier5.35184arbitrary
i0P(r)3.460.03arbitrary
mwExperimental94.6kDa
rgGuinier5.710.05Å
rgP(r)5.90.1Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris, 150 mM NaCl缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-07-18储存 / 测量温度4.0 / 25.0
曝光时间0.5帧数10
波长0.08189样品-探测器距离3.56
光源X-ray synchrotron探测器Pilatus3 S 1M, Pilatus3 900 K
机构 / 束线National Synchrotron Light Source II (NSLS-II) / 16-ID (LiX) · Upton, NY, USA
q range0.05 – 2.5样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/z5/sasdxz5/source/SASDXZ5.outnot_listedDownload查看原文件
sascifsasbdb/entries/z5/sasdxz5/source/SASDXZ5.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/z5/sasdxz5/source/summary.jsondownloaded7055564a87b499280694e2d210a30386a930aa40305c2e70485380602490113ddc53Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(1 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDXZ5
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titlePhosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.
project.publication.titlePhosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.
project.publication.author_listFavetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-025-62922-4
project.publication.pmid40858555
project.publication.published_date2025 Aug 26
project.statusreleased
project.submitted_date2025-07-03
project.released_date2025-08-19
pddf_datanull
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDXZ5.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_kratky_img.png
pddf_plotnull
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDXZ5.sascif
experiment.instrument.detector.typeDuel detector
experiment.instrument.detector.namePilatus3 S 1M, Pilatus3 900 K
experiment.instrument.detector.resolutionnull
experiment.instrument.nameNational Synchrotron Light Source II (NSLS-II)
experiment.instrument.cityUpton, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_name16-ID (LiX)
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameNucleoprotein
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
experiment.sample.molecule[0].organismSevere acute respiratory syndrome coronavirus 2
experiment.sample.molecule[0].uniprot_codeP0DTC9
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last419
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQ QQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH WPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAY KTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
experiment.sample.molecule[0].mw47.3
experiment.sample.molecule[0].total_mw94.6
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionThe protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG). N-terminal His6xtag+tev cleavage site
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSARS-CoV-2 Nucleocapsid protein 1.5 mg/mL
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameRutgers University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameBruna
experiment.contributor[0].contributor_surnameFavetta
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-3613-1665
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-07-18
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames10
experiment.wavelength0.08189
experiment.sample_detector_distance3.56
experiment.concentration_minnull
experiment.concentration_max1.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.05
experiment.s_max2.5
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareBioXTAS RAW
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of SARS-CoV-2 Nucleocapsid protein in 20 mM Tris, 150 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors. One solute concentration of 1.50 mg/ml was measured at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw94.6
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i03.46
pddf_i0_error0.03
guinier_i05.35184
guinier_i0_errornull
pddf_rg5.9
pddf_rg_error0.1
guinier_rg5.71
guinier_rg_error0.05
pddf_dmax18.8
pddf_dmax_error0.9
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last19
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDXZ5_datloglog_img.png
symmetrynull
last_modified2025-09-01T15:11:14.749430+02:00
bragg_peak[]
manifest.json:31 个字段值
字段路径原始值
codeSASDXZ5
statussuccess
started_at2026-08-11T15:00:01.878955+00:00
finished_at2026-08-11T15:00:07.047191+00:00
source_last_modified2025-09-01T15:11:14.749430+02:00
files[0].statusdownloaded
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查看完整 summary.json 原文
{
  "code": "SASDXZ5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
    "publication": {
      "title": "Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states.",
      "author_list": "Favetta B, Wang H, Cubuk J, Singh A, Barai M, Ramirez C, Zheng H, Gormley AJ, Murthy NS, Dignon G, Soranno A, Shi Z, Schuster BS",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-025-62922-4",
      "pmid": "40858555",
      "published_date": "2025 Aug 26"
    },
    "status": "released",
    "submitted_date": "2025-07-03",
    "released_date": "2025-08-19"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDXZ5.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDXZ5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDXZ5.sascif",
  "experiment": {
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        "name": "Pilatus3 S 1M, Pilatus3 900 K",
        "resolution": null
      },
      "name": "National Synchrotron Light Source II (NSLS-II)",
      "city": "Upton, NY",
      "country": "USA",
      "beamline_name": "16-ID (LiX)",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
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          "long_name": "Nucleoprotein",
          "short_name": null,
          "sequence": "MHHHHHHENLYFQGSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA",
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          "uniprot_code": "P0DTC9",
          "uniprot_range_first": 2,
          "uniprot_range_last": 419,
          "oligomerization": "dimer",
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          "mw": 47.3,
          "total_mw": 94.6,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct contains an additional 14 non-native amino acids at the N-terminus including a polyhistidine tag (MHHHHHHENLYFQG).\r\nN-terminal His6xtag+tev cleavage site"
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        "comment": null,
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        "deuteration": null
      },
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      "name": "SARS-CoV-2 Nucleocapsid protein 1.5 mg/mL",
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      "absorbption": null,
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      "mixture": null
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            "address": null,
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            "webpage": null
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    "concentration_unit": null,
    "date": "2023-07-18",
    "storage_temperature": 4.0,
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    "exposure_time": 0.5,
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    "wavelength": 0.08189,
    "sample_detector_distance": 3.56,
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  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of SARS-CoV-2 Nucleocapsid protein in 20 mM Tris, 150 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) of λ = 0.08189 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SAXS data was collected over a s range of 0.05 - 31.3 nm-1 and analyzed within a range of 0.05 - 2.55 nm-1. Sample to detector distance for small angles was 3.560 m and 0.317 m for wide angles using Pilatus3X 1M and Pilatus3X 900k detectors. One solute concentration of 1.50 mg/ml was measured at 25°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 94.6,
  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
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  "porod_mw_error": null,
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  "pddf_i0_error": 0.03,
  "guinier_i0": 5.35184,
  "guinier_i0_error": null,
  "pddf_rg": 5.9,
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  "guinier_rg_error": 0.05,
  "pddf_dmax": 18.8,
  "pddf_dmax_error": 0.9,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 2,
  "guinier_point_last": 19,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDXZ5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-09-01T15:11:14.749430+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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