SASDY66

MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-08-10T11:14:32.795525+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V

浓度— – 10.0 缓冲液 / pH50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol / 7.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.1 nm曝光2.0 s × 1500

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V
查看序列
>>MEK1DDGRA24CONSTRUCTMPLLERRGVSELPPLYINPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVLEVLFQ>>ERK2T185VCONSTRUCTQGPMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLVEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
protein—–—monomer分子数 186.579 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11000[3]0.008516277–0.4958526 1/A含误差列缺失 076sasbdb/entries/66/sasdy66/source/SASDY66.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.65Å
i0Guinier31.9703arbitrary
i0P(r)32.09261arbitrary
mwExperimental91.2kDa
mwPorod83.0kDa
porod_volumePorod134.0ų
rgGuinier3.0990.055Å
rgP(r)3.13Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-06-25储存 / 测量温度20.0 / 20.0
曝光时间2.0帧数1500
波长0.1样品-探测器距离2.827
光源X-ray synchrotron探测器Pilatus3 2M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.085 – 4.959样品体积 / 流速45.0 / 0.5

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/66/sasdy66/source/SASDY66.datdownloaded5225028adba679b15012e5e9d2f2ee8fdc0f3d47736fcf9fae1f36d7f833091754344下载查看原文件源站
full_entry_zipsasbdb/entries/66/sasdy66/source/SASDY66.zipdownloaded17506837f046ef6b8deb4721dfdfa10ba9344fcab531215afbc17edef96053813f3348下载查看原文件源站
pddfsasbdb/entries/66/sasdy66/source/SASDY66.outdownloaded85276a2cfd421ff4fb4880c54eb05d66428ac01480457bb3b0e12b81749313ab52354下载查看原文件源站
sascifsasbdb/entries/66/sasdy66/source/SASDY66.sascifnot_available下载查看原文件源站
summarysasbdb/entries/66/sasdy66/source/summary.jsondownloaded735636ab6fb6e9c076251a97f3ca2d6480fb4e99ab7e7bc892233f2c154c267d5f27下载查看原文件源站
curve:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.dat",
  "role": "curve",
  "sha256": "28adba679b15012e5e9d2f2ee8fdc0f3d47736fcf9fae1f36d7f833091754344",
  "size": 52250,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDY66.dat"
}
full_entry_zip:来源记录与 ZIP 内部目录(4 项)
{
  "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.zip",
  "role": "full_entry_zip",
  "sha256": "37f046ef6b8deb4721dfdfa10ba9344fcab531215afbc17edef96053813f3348",
  "size": 175068,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDY66.zip",
  "zip_member_count": 4,
  "zip_members": [
    {
      "crc32": "486fde4f",
      "name": "SASDY66/experimental_data/SASDY66.dat",
      "size": 52250
    },
    {
      "crc32": "8b853003",
      "name": "SASDY66/pddf/SASDY66.ift",
      "size": 85276
    },
    {
      "crc32": "a9bf7a02",
      "name": "SASDY66/SASDY66_fit1.fit",
      "size": 61973
    },
    {
      "crc32": "00f404e3",
      "name": "SASDY66/SASDY66_fit1_model1.pdb",
      "size": 502168
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.out",
  "role": "pddf",
  "sha256": "a2cfd421ff4fb4880c54eb05d66428ac01480457bb3b0e12b81749313ab52354",
  "size": 85276,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDY66.ift"
}
sascif:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.sascif",
  "role": "sascif",
  "status": "not_available",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDY66.sascif"
}
summary:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/summary.json",
  "sha256": "36ab6fb6e9c076251a97f3ca2d6480fb4e99ab7e7bc892233f2c154c267d5f27",
  "size": 7356,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDY66/"
}

全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDY66
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleMolecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1
project.publication.titleMolecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1
project.publication.author_listvon Velsen J, Juyoux P, Piasentin N, Fisher H, Lapouge K, Vadas O, Gervasio F, Bowler M
project.publication.journalnull
project.publication.doi10.7554/eLife.111925.1
project.publication.pmidnull
project.publication.published_date2026 Aug 07
project.statusreleased
project.submitted_date2026-01-18
project.released_date2026-08-10
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDY66.ift
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDY66.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDY66_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDY66.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 2M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometrypoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V
experiment.sample.molecule[0].short_nameMEK1DDGRA-ERK2T185V
experiment.sample.molecule[0].sequence>>MEK1DDGRA24 construct MPLLERRGVSELPPLYINPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVLEVLFQ >>ERK2T185V construct QGPMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLVEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw86.579
experiment.sample.molecule[0].total_mw86.579
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameESRF
experiment.contributor[0].affiliation[0].addressGrenoble, France
experiment.contributor[0].affiliation[0].full_nameEuropean Synchrotron Radiation Facility
experiment.contributor[0].affiliation[0].webpagehttp://www.esrf.eu/
experiment.contributor[0].contributor_nameHayden
experiment.contributor[0].contributor_surnameFisher
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-0093-0921
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-06-25
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time2.0
experiment.number_of_frames1500
experiment.wavelength0.1
experiment.sample_detector_distance2.827
experiment.concentration_minnull
experiment.concentration_max10.0
experiment.sample_volume45.0
experiment.flow_rate0.5
experiment.s_min0.085
experiment.s_max4.959
experiment.total_exposure_timenull
experiment.seccolumn1
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDY66_fit1_model1_img.png
fits[0].models[0].softwareOther
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelother
fits[0].models[0].software_versionNOLB
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDY66_fit1_model1.pdb
fits[0].models[0].model_mwnull
fits[0].models[0].bead_radiusnull
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/inactive_full_MODEL-4_trimmed.log
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user407
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDY66_fit1_fixed_fit_img.png
fits[0].softwarePepsi-SAXS
fits[0].chi_square_value2.7018
fits[0].p_value0.0
fits[0].fit_residual_plotSASDY66_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDY66_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/inactive_full_MODEL-4_trimmed.log
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareBioXTAS RAW
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw91.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw83.0
porod_mw_errornull
pddf_i032.09261
pddf_i0_errornull
guinier_i031.9703
guinier_i0_errornull
pddf_rg3.13
pddf_rg_errornull
guinier_rg3.099
guinier_rg_error0.055
pddf_dmax11.65
pddf_dmax_errornull
porod_volume134.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first17
guinier_point_last69
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDY66_datloglog_img.png
symmetrynull
last_modified2026-08-10T11:14:32.795525+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDY66
statussuccess
started_at2026-08-11T15:02:58.616035+00:00
finished_at2026-08-11T15:03:06.340228+00:00
source_last_modified2026-08-10T11:14:32.795525+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDY66/
files[0].path/data/saxsdb/sasbdb/entries/66/sasdy66/source/summary.json
files[0].size7356
files[0].sha25636ab6fb6e9c076251a97f3ca2d6480fb4e99ab7e7bc892233f2c154c267d5f27
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDY66.dat
files[1].path/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.dat
files[1].size52250
files[1].sha25628adba679b15012e5e9d2f2ee8fdc0f3d47736fcf9fae1f36d7f833091754344
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDY66.ift
files[2].path/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.out
files[2].size85276
files[2].sha256a2cfd421ff4fb4880c54eb05d66428ac01480457bb3b0e12b81749313ab52354
files[2].rolepddf
files[3].statusnot_available
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDY66.sascif
files[3].path/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.sascif
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDY66.zip
files[4].path/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.zip
files[4].size175068
files[4].sha25637f046ef6b8deb4721dfdfa10ba9344fcab531215afbc17edef96053813f3348
files[4].rolefull_entry_zip
files[4].zip_member_count4
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDY66",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Molecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1",
    "publication": {
      "title": "Molecular basis of mitogen-activated protein kinase ERK2 activation by its upstream kinase MEK1",
      "author_list": "von Velsen J, Juyoux P, Piasentin N, Fisher H, Lapouge K, Vadas O, Gervasio F, Bowler M",
      "journal": null,
      "doi": "10.7554/eLife.111925.1",
      "pmid": null,
      "published_date": "2026 Aug 07"
    },
    "status": "released",
    "submitted_date": "2026-01-18",
    "released_date": "2026-08-10"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDY66.ift",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDY66.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDY66_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDY66_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDY66.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V",
          "short_name": "MEK1DDGRA-ERK2T185V",
          "sequence": ">>MEK1DDGRA24 construct\r\nMPLLERRGVSELPPLYINPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGVLEVLFQ\r\n>>ERK2T185V construct\r\nQGPMAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLVEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 86.579,
          "total_mw": 86.579,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
        }
      ],
      "buffer": {
        "name": "50 mM HEPES, 200 mM NaCl, 10 mM MgCl2, 0.5 mM TCEP, 2.5% Glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "MAPK/ERK Kinase (MEK1) DD GRA24 KIM - Extracellular signal-Regulated Kinase (ERK2) T185V",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "ESRF",
            "address": "Grenoble, France",
            "full_name": "European Synchrotron Radiation Facility",
            "webpage": "http://www.esrf.eu/"
          }
        ],
        "contributor_name": "Hayden",
        "contributor_surname": "Fisher",
        "orcid": "https://orcid.org/0000-0003-0093-0921"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-06-25",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 2.0,
    "number_of_frames": 1500,
    "wavelength": 0.1,
    "sample_detector_distance": 2.827,
    "concentration_min": null,
    "concentration_max": 10.0,
    "sample_volume": 45.0,
    "flow_rate": 0.5,
    "s_min": 0.085,
    "s_max": 4.959,
    "total_exposure_time": null,
    "seccolumn": 1
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDY66_fit1_model1_img.png",
          "software": "Other",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "other",
          "software_version": "NOLB",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDY66_fit1_model1.pdb",
          "model_mw": null,
          "bead_radius": null,
          "log": "https://www.sasbdb.org/media/log_files/inactive_full_MODEL-4_trimmed.log",
          "symmetry": null,
          "comment": null,
          "user": 407
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDY66_fit1_fixed_fit_img.png",
      "software": "Pepsi-SAXS",
      "chi_square_value": 2.7018,
      "p_value": 0.0,
      "fit_residual_plot": "SASDY66_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDY66_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/inactive_full_MODEL-4_trimmed.log",
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "BioXTAS RAW",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 91.2,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 83.0,
  "porod_mw_error": null,
  "pddf_i0": 32.09261,
  "pddf_i0_error": null,
  "guinier_i0": 31.9703,
  "guinier_i0_error": null,
  "pddf_rg": 3.13,
  "pddf_rg_error": null,
  "guinier_rg": 3.099,
  "guinier_rg_error": 0.055,
  "pddf_dmax": 11.65,
  "pddf_dmax_error": null,
  "porod_volume": 134.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 17,
  "guinier_point_last": 69,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDY66_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-08-10T11:14:32.795525+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDY66",
  "status": "success",
  "started_at": "2026-08-11T15:02:58.616035+00:00",
  "finished_at": "2026-08-11T15:03:06.340228+00:00",
  "source_last_modified": "2026-08-10T11:14:32.795525+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDY66/",
      "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/summary.json",
      "size": 7356,
      "sha256": "36ab6fb6e9c076251a97f3ca2d6480fb4e99ab7e7bc892233f2c154c267d5f27"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDY66.dat",
      "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.dat",
      "size": 52250,
      "sha256": "28adba679b15012e5e9d2f2ee8fdc0f3d47736fcf9fae1f36d7f833091754344",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDY66.ift",
      "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.out",
      "size": 85276,
      "sha256": "a2cfd421ff4fb4880c54eb05d66428ac01480457bb3b0e12b81749313ab52354",
      "role": "pddf"
    },
    {
      "status": "not_available",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDY66.sascif",
      "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.sascif",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDY66.zip",
      "path": "/data/saxsdb/sasbdb/entries/66/sasdy66/source/SASDY66.zip",
      "size": 175068,
      "sha256": "37f046ef6b8deb4721dfdfa10ba9344fcab531215afbc17edef96053813f3348",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}