SASDY96

Chordin fragment aa.168-650

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-06-15T09:04:33.907949+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Chordin fragment aa.168-650

浓度— – 9.0 缓冲液 / pH25 mM HEPES, 150 mM NaCl / 7.5
Experimental temperature15.0 设备 / 束线Diamond Light Source / B21
波长0.09464 nm曝光1.0 s × 600

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Chordin fragment aa.168-650
查看序列
APLAGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFAEVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQTGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHTAVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAGALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLKGFYGSEAQGVVKDLEPELLRHLAKGMASLLITTKGSPRGELRGQVHIANQCELVPRGSHHHHHH
proteinHomo sapiensQ9H2X0168–644monomer分子数 153.6 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12573[3]0.0045–0.34 1/A含误差列缺失 044sasbdb/entries/96/sasdy96/source/SASDY96.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.57Å
i0Guinier0.1158451/cm
i0P(r)0.11591/cm
mwExperimental61.3kDa
mwPorod66.0kDa
porod_volumePorod106.0ų
rgGuinier2.9620.02Å
rgP(r)2.98Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

拟合 1 · MultiFoXS main.af0ac5f

χ²:2.054974CorMap p:0.0单位:1/A
模型名称 / 类型软件Symmetry模型 MWPDB 声明来源文件
1atomicMultiFoXS multi_state_model_2_1_1.datC150.99模型 图片
2atomicMultiFoXS multi_state_model_2_1_1.datC150.99模型 图片

拟合 2 · MultiFoXS main.af0ac5f

χ²:2.277442CorMap p:0.0单位:1/A
模型名称 / 类型软件Symmetry模型 MWPDB 声明来源文件
1atomicMultiFoXS main.af0ac5fC150.99模型 图片

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM HEPES, 150 mM NaCl缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-07-26储存 / 测量温度-80.0 / 15.0
曝光时间1.0帧数600
波长0.09464样品-探测器距离3.6883
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4样品体积 / 流速50.0 / 0.07

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/96/sasdy96/source/SASDY96.datdownloaded1060397e120127290d334718ab38cdcd240b701cebee7f6c3f5c554bbd82bcf4441232Download查看原文件源站
full_entry_zipsasbdb/entries/96/sasdy96/source/SASDY96.zipdownloaded378071ca9df43574f1077eca1cdf6f57698b5c673494b5a402427a7fcccf9f0adbcf4aDownload查看原文件源站
pddfsasbdb/entries/96/sasdy96/source/SASDY96.outdownloaded167249848f7c52d3cc6baaebcf95f1d193884046921ce1198f4e8161e3ea89ce8adfc4Download查看原文件源站
sascifsasbdb/entries/96/sasdy96/source/SASDY96.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/96/sasdy96/source/summary.jsondownloaded9403b18351cd7bab0134ab6919f4faa80816b405d3f4aa1bb4934cf02cc64021f9a1Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(7 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:192 个字段值
字段路径原始值
codeSASDY96
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructural and biophysical analysis of the four CHRD domains of human chordin reveals a novel binding site for glycosaminoglycans.
project.publication.titleStructural and biophysical analysis of the four CHRD domains of human chordin reveals a novel binding site for glycosaminoglycans.
project.publication.author_listSnee M, Birchenough HL, Becker MH, Popplewell JF, Ashe HL, Baldock C
project.publication.journalJ Biol Chem
project.publication.doi10.1016/j.jbc.2026.113248
project.publication.pmid42285510
project.publication.published_date2026 Jun 12
project.statusreleased
project.submitted_date2026-01-27
project.released_date2026-06-15
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experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameChordin fragment aa.168-650
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceAPLAGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFAEVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQTGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHTAVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAGALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLKGFYGSEAQGVVKDLEPELLRHLAKGMASLLITTKGSPRGELRGQVHIANQCELVPRGSHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9H2X0
experiment.sample.molecule[0].uniprot_range_first168
experiment.sample.molecule[0].uniprot_range_last644
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPSLPAPPAPLLLLGLLLLGSRPARGAGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYAL DETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGH CCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPR SQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRA VPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGI TLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFA EVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQ TGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHT AVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAG ALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLK GFYGSEAQGVVKDLEPELLRHLAKGMASLMITTKGSPRGELRGQVHIANQCEVGGLRLEA AGAEGVRALGAPDTASAAPPVVPGLPALAPAKPGGPGRPRDPNTCFFEGQQRPHGARWAP NYDPLCSLCTCQRRTVICDPVVCPPPSCPHPVQAPDQCCPVCPEKQDVRDLPGLPRSRDP GEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGGTGEVHCEKVQCPRLACAQPVR VNPTDCCKQCPVGSGAHPQLGDPMQADGPRGCRFAGQWFPESQSWHPSVPPFGEMSCITC RCGAGVPHCERDDCSLPLSCGSGKESRCCSRCTAHRRPAPETRTDPELEKEAEGS
experiment.sample.molecule[0].mw53.6
experiment.sample.molecule[0].total_mw53.6
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name25 mM HEPES, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameChordin fragment aa.168-650
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressManchester, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Manchester
experiment.contributor[0].affiliation[0].webpagehttp://www.manchester.ac.uk/
experiment.contributor[0].contributor_nameMark H.
experiment.contributor[0].contributor_surnameBecker
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-3474-2419
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-07-26
experiment.storage_temperature-80.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
experiment.number_of_frames600
experiment.wavelength0.09464
experiment.sample_detector_distance3.6883
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experiment.concentration_max9.0
experiment.sample_volume50.0
experiment.flow_rate0.07
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumn48
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fits[0].fit_residual_plotSASDY96_fit1_fitfoxsresiduals_img.png
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pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw61.3
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw66.0
porod_mw_errornull
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pddf_rg_errornull
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guinier_rg_error0.02
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pddf_dmax_errornull
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porod_volume_errornull
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estimated_volume_errornull
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intensities_log_log_plotSASDY96_datloglog_img.png
symmetrynull
last_modified2026-06-15T09:04:33.907949+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDY96
statussuccess
started_at2026-08-11T15:04:47.188051+00:00
finished_at2026-08-11T15:04:56.131829+00:00
source_last_modified2026-06-15T09:04:33.907949+02:00
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查看完整 summary.json 原文
{
  "code": "SASDY96",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural and biophysical analysis of the four CHRD domains of human chordin reveals a novel binding site for glycosaminoglycans.",
    "publication": {
      "title": "Structural and biophysical analysis of the four CHRD domains of human chordin reveals a novel binding site for glycosaminoglycans.",
      "author_list": "Snee M, Birchenough HL, Becker MH, Popplewell JF, Ashe HL, Baldock C",
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      "pmid": "42285510",
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查看完整 manifest.json 原文
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