SASDY97

Alkaline phosphatase (ALP) under vis

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-03-16T10:30:41.880631+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Alkaline phosphatase (ALP) under vis

浓度— – 1.0 缓冲液 / pH25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerol / 7.5
Experimental temperature20.0 设备 / 束线Shanghai Synchrotron Radiation Facility (SSRF) / BL19U2
波长0.1033 nm曝光0.2 s × 20

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Wild Alkaline Phosphatase under vis
查看序列
MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
proteinEscherichia coliP00634—–—dimer分子数 —48.68 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11060[3]0.0143023413–0.429266163 1/A含误差列缺失 014sasbdb/entries/97/sasdy97/source/SASDY97.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)9.0Å
i0Guinier26.18arbitrary
i0P(r)26.39arbitrary
mwExperimental97.367kDa
mwPorod62.5kDa
porod_volumePorod75.4ų
rgGuinier2.80.489Å
rgP(r)2.8Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerol缓冲液浓度
pH7.5添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-12-23储存 / 测量温度4.0 / 20.0
曝光时间0.2帧数20
波长0.1033样品-探测器距离2.6
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线Shanghai Synchrotron Radiation Facility (SSRF) / BL19U2 · Shanghai, China
q range0.143 – 4.293样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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sascifsasbdb/entries/97/sasdy97/source/SASDY97.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/97/sasdy97/source/summary.jsondownloaded719474939e9149b239162fde3b42cde63ccd160af4abf625399af04d5a46e7552335Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDY97
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSingle-engineered-residue solvation perturbations regulate global protein architecture and function.
project.publication.titleSingle-engineered-residue solvation perturbations regulate global protein architecture and function.
project.publication.author_listLiu Y, Zhai J, Cao S, Guo H, Zhang H, Song B, Guo J, Men D, He X, Li D
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-026-70155-2
project.publication.pmid41807374
project.publication.published_date2026 Mar 10
project.statusreleased
project.submitted_date2026-02-13
project.released_date2026-03-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDY97.out
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameShanghai Synchrotron Radiation Facility (SSRF)
experiment.instrument.cityShanghai
experiment.instrument.countryChina
experiment.instrument.beamline_nameBL19U2
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameWild Alkaline Phosphatase under vis
experiment.sample.molecule[0].short_nameALP-vis
experiment.sample.molecule[0].sequenceMKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codeP00634
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLS DKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDY VTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAA LVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAG EWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGN IDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQ IGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVM VMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
experiment.sample.molecule[0].mw48.68
experiment.sample.molecule[0].total_mw97.367
experiment.sample.molecule[0].number_molecules0
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameAlkaline phosphatase (ALP) under vis
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameEast China Normal University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameYINGYA
experiment.contributor[0].contributor_surnameLiu
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-12-23
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time0.2
experiment.number_of_frames20
experiment.wavelength0.1033
experiment.sample_detector_distance2.6
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.143
experiment.s_max4.293
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareCRYSOL
fits[0].chi_square_value2.098
fits[0].p_value0.0002
fits[0].fit_residual_plotSASDY97_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw97.367
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw62.5
porod_mw_errornull
pddf_i026.39
pddf_i0_errornull
guinier_i026.18
guinier_i0_errornull
pddf_rg2.8
pddf_rg_errornull
guinier_rg2.8
guinier_rg_error0.489
pddf_dmax9.0
pddf_dmax_errornull
porod_volume75.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first19
guinier_point_last44
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDY97_datloglog_img.png
symmetrynull
last_modified2026-03-16T10:30:41.880631+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDY97
statussuccess
started_at2026-08-11T15:25:04.277226+00:00
finished_at2026-08-11T15:25:15.486409+00:00
source_last_modified2026-03-16T10:30:41.880631+01:00
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查看完整 summary.json 原文
{
  "code": "SASDY97",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Single-engineered-residue solvation perturbations regulate global protein architecture and function.",
    "publication": {
      "title": "Single-engineered-residue solvation perturbations regulate global protein architecture and function.",
      "author_list": "Liu Y, Zhai J, Cao S, Guo H, Zhang H, Song B, Guo J, Men D, He X, Li D",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-70155-2",
      "pmid": "41807374",
      "published_date": "2026 Mar 10"
    },
    "status": "released",
    "submitted_date": "2026-02-13",
    "released_date": "2026-03-16"
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          "molecular_type": "protein",
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          "molecule_description": ""
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查看完整 manifest.json 原文
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