SASDY97

Alkaline phosphatase (ALP) under vis

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-03-16T10:30:41.880631+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Alkaline phosphatase (ALP) under vis

Concentration— – 1.0 Buffer / pH25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerol / 7.5
Experimental temperature20.0 Facility / beamlineShanghai Synchrotron Radiation Facility (SSRF) / BL19U2
Wavelength0.1033 nmExposure0.2 s × 20

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Wild Alkaline Phosphatase under vis
View sequence
MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
proteinEscherichia coliP00634—–—dimerMolecule count —48.68 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11060[3]0.0143023413–0.429266163 1/AHas uncertainty columnMissing 014sasbdb/entries/97/sasdy97/source/SASDY97.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.0—Å
i0Guinier26.18—arbitrary
i0P(r)26.39—arbitrary
mwExperimental97.367—kDa
mwPorod62.5—kDa
porod_volumePorod75.4—ų
rgGuinier2.80.489Å
rgP(r)2.8—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerolBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2024-12-23Storage / measurement temperature4.0 / 20.0
Exposure time0.2Frames20
Wavelength0.1033Sample-to-detector distance2.6
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineShanghai Synchrotron Radiation Facility (SSRF) / BL19U2 · Shanghai, China
q range0.143 – 4.293Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDY97
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSingle-engineered-residue solvation perturbations regulate global protein architecture and function.
project.publication.titleSingle-engineered-residue solvation perturbations regulate global protein architecture and function.
project.publication.author_listLiu Y, Zhai J, Cao S, Guo H, Zhang H, Song B, Guo J, Men D, He X, Li D
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-026-70155-2
project.publication.pmid41807374
project.publication.published_date2026 Mar 10
project.statusreleased
project.submitted_date2026-02-13
project.released_date2026-03-16
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameShanghai Synchrotron Radiation Facility (SSRF)
experiment.instrument.cityShanghai
experiment.instrument.countryChina
experiment.instrument.beamline_nameBL19U2
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameWild Alkaline Phosphatase under vis
experiment.sample.molecule[0].short_nameALP-vis
experiment.sample.molecule[0].sequenceMKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codeP00634
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLS DKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDY VTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAA LVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAG EWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGN IDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQ IGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVM VMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
experiment.sample.molecule[0].mw48.68
experiment.sample.molecule[0].total_mw97.367
experiment.sample.molecule[0].number_molecules0
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name25 mM Tris, 100 mM NaCl, 2 mM TCEP, 3% (w/v) glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameAlkaline phosphatase (ALP) under vis
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameEast China Normal University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameYINGYA
experiment.contributor[0].contributor_surnameLiu
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-12-23
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time0.2
experiment.number_of_frames20
experiment.wavelength0.1033
experiment.sample_detector_distance2.6
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.143
experiment.s_max4.293
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareCRYSOL
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fits[0].p_value0.0002
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw97.367
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw62.5
porod_mw_errornull
pddf_i026.39
pddf_i0_errornull
guinier_i026.18
guinier_i0_errornull
pddf_rg2.8
pddf_rg_errornull
guinier_rg2.8
guinier_rg_error0.489
pddf_dmax9.0
pddf_dmax_errornull
porod_volume75.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first19
guinier_point_last44
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDY97_datloglog_img.png
symmetrynull
last_modified2026-03-16T10:30:41.880631+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDY97
statussuccess
started_at2026-08-11T15:25:04.277226+00:00
finished_at2026-08-11T15:25:15.486409+00:00
source_last_modified2026-03-16T10:30:41.880631+01:00
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View the complete summary.json source
{
  "code": "SASDY97",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Single-engineered-residue solvation perturbations regulate global protein architecture and function.",
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      "title": "Single-engineered-residue solvation perturbations regulate global protein architecture and function.",
      "author_list": "Liu Y, Zhai J, Cao S, Guo H, Zhang H, Song B, Guo J, Men D, He X, Li D",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-70155-2",
      "pmid": "41807374",
      "published_date": "2026 Mar 10"
    },
    "status": "released",
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    "released_date": "2026-03-16"
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          "molecule_source": "biological",
          "molecule_description": ""
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      },
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      "name": "Alkaline phosphatase (ALP) under vis",
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View the complete manifest.json source
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