SASDYF4

Mutant Elongation Factor Like-1 protein

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-03-20T09:53:28.146532+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Mutant Elongation Factor Like-1 protein

浓度— – 6.0 缓冲液 / pH50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol / 8.0
Experimental temperature20.0 设备 / 束线Diamond Light Source / B21
波长0.095 nm曝光3.0 s × 600

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Mutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH)
查看序列
MPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDARPDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVIEQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGWGFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILENIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLTVIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREELPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDTVMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDPNDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEISVLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIEKGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHILCTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLLSQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFEEVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNGFQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAIHEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVPVVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARKHMNAIQRRKGLFIEEKVVENAEKQRTLKKNRSRSGSENLYFQGSHHHHHHHH
proteinSaccharomyces cerevisiaeP538931–1110monomer分子数 1127.1 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12213[3]0.008282854–0.2968233 1/A含误差列缺失 0195sasbdb/entries/f4/sasdyf4/source/SASDYF4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)15.2Å
i0Guinier0.0399201arbitrary
i0P(r)0.5431123arbitrary
mwExperimental191.8kDa
mwPorod191.8kDa
porod_volumePorod314.4ų
rgGuinier4.690.061Å
rgP(r)4.48Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol缓冲液浓度
pH8.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2024-10-03储存 / 测量温度4.0 / 20.0
曝光时间3.0帧数600
波长0.095样品-探测器距离3.7
光源X-ray synchrotron探测器Eiger 4M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q range0.083 – 2.968样品体积 / 流速50.0 / 0.08

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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full_entry_zipsasbdb/entries/f4/sasdyf4/source/SASDYF4.zipdownloaded30751c0b8612ec19ef6011fa2a9d3229660f628157eee08cba47a3ce9ef4e51edd88dDownload查看原文件源站
pddfsasbdb/entries/f4/sasdyf4/source/SASDYF4.outdownloaded3809ce0f3daecc946c29f60542e0b8aa01c91cd85e7f8110c7a28035c463a70e9f64Download查看原文件源站
sascifsasbdb/entries/f4/sasdyf4/source/SASDYF4.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/f4/sasdyf4/source/summary.jsondownloaded7795d452ebf72045bab32ad8fa7adefe270f83ade4cd8116bee76794054a8a0aa317Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDYF4
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleHydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.
project.publication.titleHydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.
project.publication.author_listZúñiga-Domínguez JA, Jain R, González-Andrade M, Farquhar ER, Chance MR, Gijsbers A, Sánchez-Puig N
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.70504
project.publication.pmid41848393
project.publication.published_date2026 Apr
project.statusreleased
project.submitted_date2025-12-22
project.released_date2026-03-20
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDYF4.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYF4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYF4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYF4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYF4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYF4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDYF4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH)
experiment.sample.molecule[0].short_nameEFL1 R1086Q
experiment.sample.molecule[0].sequenceMPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDARPDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVIEQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGWGFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILENIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLTVIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREELPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDTVMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDPNDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEISVLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIEKGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHILCTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLLSQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFEEVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNGFQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAIHEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVPVVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARKHMNAIQRRKGLFIEEKVVENAEKQRTLKKNRSRSGSENLYFQGSHHHHHHHH
experiment.sample.molecule[0].organismSaccharomyces cerevisiae
experiment.sample.molecule[0].uniprot_codeP53893
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last1110
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDAR PDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLC DGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVI EQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGW GFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILE NIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLT VIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREE LPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDT VMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDP NDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEIS VLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIE KGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHIL CTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLL SQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFE EVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNG FQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAI HEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVP VVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARK HMNAIRRRKGLFIEEKVVENAEKQRTLKKN
experiment.sample.molecule[0].mw127.1
experiment.sample.molecule[0].total_mw127.1
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionSaccharomyces cerevisiae mutante R1086Q EFL1 produced recombinantly in S. cerevisiae
experiment.sample.buffer.name50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMutant Elongation Factor Like-1 protein
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversidad Nacional Autónoma de México
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNuria
experiment.contributor[0].contributor_surnameSanchez Puig
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-8945-7001
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-10-03
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.095
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max6.0
experiment.sample_volume50.0
experiment.flow_rate0.08
experiment.s_min0.083
experiment.s_max2.968
experiment.total_exposure_timenull
experiment.seccolumn19
fits[]
estimated_volume_methodnull
pddf_softwareScÅtter
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw191.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw191.8
porod_mw_errornull
pddf_i00.5431123
pddf_i0_errornull
guinier_i00.0399201
guinier_i0_errornull
pddf_rg4.48
pddf_rg_errornull
guinier_rg4.69
guinier_rg_error0.061
pddf_dmax15.2
pddf_dmax_errornull
porod_volume314.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first24
guinier_point_last131
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYF4_datloglog_img.png
symmetrynull
last_modified2026-03-20T09:53:28.146532+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYF4
statussuccess
started_at2026-08-11T16:30:46.889639+00:00
finished_at2026-08-11T16:30:53.917179+00:00
source_last_modified2026-03-20T09:53:28.146532+01:00
files[0].statusdownloaded
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查看完整 summary.json 原文
{
  "code": "SASDYF4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.",
    "publication": {
      "title": "Hydroxyl radical footprinting modification reveals an intradomain communication pathway in EFL1 disrupted by a Shwachman-Diamond syndrome-associated mutation.",
      "author_list": "Zúñiga-Domínguez JA, Jain R, González-Andrade M, Farquhar ER, Chance MR, Gijsbers A, Sánchez-Puig N",
      "journal": "Protein Sci",
      "doi": "10.1002/pro.70504",
      "pmid": "41848393",
      "published_date": "2026 Apr"
    },
    "status": "released",
    "submitted_date": "2025-12-22",
    "released_date": "2026-03-20"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYF4.dat",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYF4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYF4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYF4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYF4_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYF4.sascif",
  "experiment": {
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        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Mutated ribosome assembly protein 1 (R1086Q) with C-terminal tag (RSRSGSENLYFQGSHHHHHHHH)",
          "short_name": "EFL1 R1086Q",
          "sequence": "MPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDARPDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVIEQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGWGFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILENIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLTVIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREELPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDTVMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDPNDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEISVLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIEKGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHILCTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLLSQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFEEVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNGFQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAIHEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVPVVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARKHMNAIQRRKGLFIEEKVVENAEKQRTLKKNRSRSGSENLYFQGSHHHHHHHH",
          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "P53893",
          "uniprot_range_first": 1,
          "uniprot_range_last": 1110,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPRVESETYKRLQNDPSCIRNICIVAHVDHGKTSLSDSLLASNGIISQRLAGKIRFLDAR\nPDEQLRGITMESSAISLYFRVLRKQEGSDEPLVSEHLVNLIDSPGHIDFSSEVSAASRLC\nDGAVVLVDVVEGVCSQTVTVLRQCWTEKLKPILVLNKIDRLITELQLTPQEAYIHLSKVI\nEQVNSVIGSFFANERQLDDLFWREQLEKNENAEYIEKDDSGIYFNPTDNNVIFASAIDGW\nGFNIGQLAKFYEQKLGAKRENLQKVLWGDFYMDPKTKKIINNKGLKGRSLKPLFTSLILE\nNIWKIYQNIITSRDSEMVEKIAKTLNIKLLARDLRSKDDKQLLRTIMGQWLPVSTAVLLT\nVIEKLPSPLESQTDRLNTILVSESDTAAMDPRLLKAMKTCDKEGPVSAYVSKMLSIPREE\nLPVESKRIASSDELMERSRKAREEALNAAKHAGMVENMAMMDLNDNSKNTSDLYKRAKDT\nVMTPEVGEQTKPKPSRNNDVFCVVSEPSSALDLEFEYEGEDDSDSQDNFGLDFVPTDIDP\nNDPLSSMFEYEEEDPLLESIKQISEDVNDEVDDIFDEKEECLVAFARIYSGTLRVGQEIS\nVLGPKYDPKCPEEHIETAIITHLYLFMGKELVPLDVCPSGNIVGIRGLAGKVLKSGTLIE\nKGVQGVNLAGVNFHFTPIVRVAVEPANPVEMSKLVRGLKLLDQADPCVHTYVENTGEHIL\nCTAGELHLERCLKDLTERFAGIEITHSEPAIPYRETFLSASDMNPPQNSQLGRGVHELLL\nSQYKITFRTFPLSGKVTDFLSQHQNSIKNILKTSTSSMDPVIESTGSSFLDKKSLLVAFE\nEVINQEEKSRELLSGFKVKLAGFGPSRVGCNILLSQDNLLGSLFEGTPAAFEYSDSIKNG\nFQLAVSEGPLANEPVQGMCVLVESVHKMSQDEIESIEDPRYQQHIVDLSGRLITSTRDAI\nHEAFLDWSPRIMWAIYSCDIQTSVDVLGKVYAVILQRHGKIISEEMKEGTPFFQIEAHVP\nVVEAFGLSEDIRKRTSGAAQPQLVFSGFECIDLDPFWVPTTEEELEELGDTADRENIARK\nHMNAIRRRKGLFIEEKVVENAEKQRTLKKN",
          "mw": 127.1,
          "total_mw": 127.1,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Saccharomyces cerevisiae mutante R1086Q EFL1 produced recombinantly in S. cerevisiae"
        }
      ],
      "buffer": {
        "name": "50 mM HEPES, 300 mM NaCl, 5 mM MgCl2, 5% glycerol",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Mutant Elongation Factor Like-1 protein",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Universidad Nacional Autónoma de México",
            "webpage": null
          }
        ],
        "contributor_name": "Nuria",
        "contributor_surname": "Sanchez Puig",
        "orcid": "https://orcid.org/0000-0002-8945-7001"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2024-10-03",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 3.0,
    "number_of_frames": 600,
    "wavelength": 0.095,
    "sample_detector_distance": 3.7,
    "concentration_min": null,
    "concentration_max": 6.0,
    "sample_volume": 50.0,
    "flow_rate": 0.08,
    "s_min": 0.083,
    "s_max": 2.968,
    "total_exposure_time": null,
    "seccolumn": 19
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ScÅtter",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 191.8,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 191.8,
  "porod_mw_error": null,
  "pddf_i0": 0.5431123,
  "pddf_i0_error": null,
  "guinier_i0": 0.0399201,
  "guinier_i0_error": null,
  "pddf_rg": 4.48,
  "pddf_rg_error": null,
  "guinier_rg": 4.69,
  "guinier_rg_error": 0.061,
  "pddf_dmax": 15.2,
  "pddf_dmax_error": null,
  "porod_volume": 314.4,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 24,
  "guinier_point_last": 131,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDYF4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-20T09:53:28.146532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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  "finished_at": "2026-08-11T16:30:53.917179+00:00",
  "source_last_modified": "2026-03-20T09:53:28.146532+01:00",
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