SASDYJ2

Hen Egg White Lysozyme pH 7.4

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-11-12T08:45:15.794651+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Hen Egg White Lysozyme pH 7.4

Concentration— – 16.0 Buffer / pH20 mM HEPES, 150 mM NaCl / 7.4
Experimental temperature15.0 Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2
Wavelength0.1127 nmExposure1.0 s × 16

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Hen egg white lysozyme
View sequence
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
proteinGallus gallusP0069819–147monomerMolecule count 114.314 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1964[3]0.01025133–0.7479287 1/AHas uncertainty columnMissing 05sasbdb/entries/j2/sasdyj2/source/SASDYJ2.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)59.0—Å
i0Guinier125.838—arbitrary
i0P(r)126.00.11arbitrary
mwExperimental10.9—kDa
mwGuinier I(0)10.9—kDa
mwPorod14.0—kDa
porod_volumePorod23.0—ų
rgGuinier1.5810.022Å
rgP(r)1.5980.003Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

Fit 2 · DENSS

χ²: 4.2625CorMap p: 0.0Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—densityDENSS ———Model
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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 150 mM NaClBuffer concentration—
pH7.4Additives—
Buffer descriptionExtensively dialyzed
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-07-09Storage / measurement temperature15.0 / 15.0
Exposure time1.0Frames16
Wavelength0.1127Sample-to-detector distance1.2486
SourceX-ray synchrotronDetectorPilatus3 X 1M
Facility / beamlineStanford Synchrotron Radiation Lightsource (SSRL) / BL4-2 · Menlo Park, CA, USA
q range0.103 – 7.479Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:174 field values
Field pathRaw value
codeSASDYJ2
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleLysozyme pH 7.4 with models
project.publicationnull
project.statusreleased
project.submitted_date2025-11-05
project.released_date2025-11-12
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experiment.instrument.detector.namePilatus3 X 1M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameStanford Synchrotron Radiation Lightsource (SSRL)
experiment.instrument.cityMenlo Park, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBL4-2
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHen egg white lysozyme
experiment.sample.molecule[0].short_nameLyszoyme
experiment.sample.molecule[0].sequenceKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
experiment.sample.molecule[0].organismGallus gallus
experiment.sample.molecule[0].uniprot_codeP00698
experiment.sample.molecule[0].uniprot_range_first19
experiment.sample.molecule[0].uniprot_range_last147
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQA TNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDG NGMNAWVAWRNRCKGTDVQAWIRGCRL
experiment.sample.molecule[0].mw14.314
experiment.sample.molecule[0].total_mw14.314
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionHen Egg White Lysozyme, purchased from Sigma
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentExtensively dialyzed
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHen Egg White Lysozyme pH 7.4
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameTopos Bio
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameAnthony
experiment.contributor[0].contributor_surnameGiannetti
experiment.contributor[0].orcidhttps://orcid.org/0009-0003-4487-4826
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-07-09
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time1.0
experiment.number_of_frames16
experiment.wavelength0.1127
experiment.sample_detector_distance1.2486
experiment.concentration_minnull
experiment.concentration_max16.0
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experiment.flow_ratenull
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experiment.total_exposure_timenull
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experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw10.9
experimental_mw_errornull
guinier_i0_mw10.9
guinier_i0_mw_errornull
porod_mw14.0
porod_mw_errornull
pddf_i0126.0
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guinier_i0125.838
guinier_i0_errornull
pddf_rg1.598
pddf_rg_error0.003
guinier_rg1.581
guinier_rg_error0.022
pddf_dmax59.0
pddf_dmax_errornull
porod_volume23.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first19
guinier_point_last93
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYJ2_datloglog_img.png
symmetrynull
last_modified2025-11-12T08:45:15.794651+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDYJ2
statussuccess
started_at2026-08-11T13:40:01.576243+00:00
finished_at2026-08-11T13:40:10.827971+00:00
source_last_modified2025-11-12T08:45:15.794651+01:00
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View the complete summary.json source
{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
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