SASDYL2

Human prothrombin in the unbound state

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-07-29T11:49:25.434245+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human prothrombin in the unbound state

Concentration— – 0.22 Buffer / pH10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol, / 7.4
Experimental temperature20.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.095 nmExposure1.0 s × 21

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Human prothrombin
View sequence
ANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE
proteinHomo sapiensP0073444–622monomerMolecule count 172.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12567[3]0.0045–0.34 1/AHas uncertainty columnMissing 0611sasbdb/entries/l2/sasdyl2/source/SASDYL2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.0—Å
i0Guinier0.0144486—1/cm
i0P(r)0.01433—1/cm
mwExperimental67.089—kDa
mwPorod77.0—kDa
porod_volumePorod123.0—ų
rgGuinier3.420.01Å
rgP(r)3.410.12Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,Buffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2022-04-29Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames21
Wavelength0.095Sample-to-detector distance3.7
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:185 field values
Field pathRaw value
codeSASDYL2
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleProthrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers.
project.publication.titleProthrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers.
project.publication.author_listTroisi R, Cangiano A, Cowieson N, Spiridonova V, Pozzi N, Del Vecchio P, Paduano L, Sica F
project.publication.journalMol Ther
project.publication.doi10.1016/j.ymthe.2026.07.042
project.publication.pmid42494063
project.publication.published_date2026 Jul 23
project.statusreleased
project.submitted_date2025-11-08
project.released_date2026-07-29
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman prothrombin
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP00734
experiment.sample.molecule[0].uniprot_range_first44
experiment.sample.molecule[0].uniprot_range_last622
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLEREC VEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHV NITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQE CSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASA QAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETG DGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYI DGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTEN DLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHP VCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDST RIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKY GFYTHVFRLKKWIQKVIDQFGE
experiment.sample.molecule[0].mw72.0
experiment.sample.molecule[0].total_mw72.0
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name10 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 5 mM CaCl2, 1% v/v glycerol,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman prothrombin in the unbound state
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversity of Naples Federico II
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameRomualdo
experiment.contributor[0].contributor_surnameTroisi
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-3484-3589
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2022-04-29
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames21
experiment.wavelength0.095
experiment.sample_detector_distance3.7
experiment.concentration_minnull
experiment.concentration_max0.22
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radiusnull
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fits[0].models[0].commentHuman prothrombin in the open conformation
fits[0].models[0].user1290
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fits[0].p_value0.7151
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fits[1].models[0].bead_radiusnull
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fits[1].models[0].user1290
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fits[1].p_value0.7151
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pddf_software_version3.1.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw67.089
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
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guinier_rg3.42
guinier_rg_error0.01
pddf_dmax12.0
pddf_dmax_errornull
porod_volume123.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first2
guinier_point_last169
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYL2_datloglog_img.png
symmetrynull
last_modified2026-07-29T11:49:25.434245+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDYL2
statussuccess
started_at2026-08-11T13:41:29.438618+00:00
finished_at2026-08-11T13:41:42.926552+00:00
source_last_modified2026-07-29T11:49:25.434245+02:00
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View the complete summary.json source
{
  "code": "SASDYL2",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Prothrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers.",
    "publication": {
      "title": "Prothrombin recognition and conformational modulation by anti-thrombin anticoagulant aptamers.",
      "author_list": "Troisi R, Cangiano A, Cowieson N, Spiridonova V, Pozzi N, Del Vecchio P, Paduano L, Sica F",
      "journal": "Mol Ther",
      "doi": "10.1016/j.ymthe.2026.07.042",
      "pmid": "42494063",
      "published_date": "2026 Jul 23"
    },
    "status": "released",
    "submitted_date": "2025-11-08",
    "released_date": "2026-07-29"
  },
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      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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      "xray_energy": null,
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    },
    "sample": {
      "molecule": [
        {
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View the complete manifest.json source
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