SASDYM4

Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (4 mg/ml)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-03-26T13:49:38.423216+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (4 mg/ml)

浓度— – 4.0 缓冲液 / pH20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP / 7.5
实验温度20.0 设备 / 束线PETRA III / EMBL P12
波长0.123979 nm曝光0.095 s × 40

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Heat shock cognate 71 kDa protein
查看序列
SNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
proteinP111421–646other分子数 171.142 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12593[3]0.002247303–0.7247599 1/A含误差列缺失 0113sasbdb/entries/m4/sasdym4/source/SASDYM4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)18.4Å
i0Guinier0.0712431/cm
i0P(r)0.072591/cm
mwExperimental92.0kDa
mwPorod100.0kDa
porod_volumePorod160.0ų
rgGuinier4.3080.019Å
rgP(r)4.63Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP缓冲液浓度
pH7.5添加剂
缓冲液说明HEPES buffer with ATP
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-03-06储存 / 测量温度4.0 / 20.0
曝光时间0.095帧数40
波长0.123979样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.022 – 7.248样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/m4/sasdym4/source/SASDYM4.zipdownloaded49902613f0fff96f11c9ba7406106bf2f06ebf4831cd53cde413e72ee79d8fe4db3fc下载查看原文件源站
pddfsasbdb/entries/m4/sasdym4/source/SASDYM4.outdownloaded62961572ad3205b2892b18d93a4f68963c098c257a5113d88dddd27b408ce1b1a06a1下载查看原文件源站
sascifsasbdb/entries/m4/sasdym4/source/SASDYM4.sascifnot_available下载查看原文件源站
summarysasbdb/entries/m4/sasdym4/source/summary.jsondownloaded6525bb2eac80e878fcccb842ccc7d6d160b9d0e5f009778dfb94de1e4f1f4c0f0f25下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDYM4
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleAdvances on the large-scale bacterial production and stable phosphorylation of Hsc70
project.publication.titleAdvances on the large-scale bacterial production and stable phosphorylation of Hsc70
project.publication.author_listKley N, Hecht-Bucher M, Itzen A
project.publication.journalProtein Expression and Purification
project.publication.doi10.1016/j.pep.2026.106923
project.publication.pmidnull
project.publication.published_date2026 Mar
project.statusreleased
project.submitted_date2026-01-12
project.released_date2026-03-26
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDYM4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYM4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYM4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDYM4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHeat shock cognate 71 kDa protein
experiment.sample.molecule[0].short_nameHsc70
experiment.sample.molecule[0].sequenceSNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeP11142
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last646
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA MNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVS SMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAA IAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNH FIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRA RFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELN KSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTI PTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDI DANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKN SLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELE KVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
experiment.sample.molecule[0].mw71.142
experiment.sample.molecule[0].total_mw71.142
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1 mM TCEP, 1 mM ATP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentHEPES buffer with ATP
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHeat shock cognate 71 kDa protein (Hsc70) T204A mutant with ATP (4 mg/ml)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHamburg, Germany
experiment.contributor[0].affiliation[0].full_nameUniversity of Hamburg
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-hamburg.de/
experiment.contributor[0].contributor_nameNicholas
experiment.contributor[0].contributor_surnameKley
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-03-06
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time0.095
experiment.number_of_frames40
experiment.wavelength0.123979
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.022
experiment.s_max7.248
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw92.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw100.0
porod_mw_errornull
pddf_i00.07259
pddf_i0_errornull
guinier_i00.071243
guinier_i0_errornull
pddf_rg4.63
pddf_rg_errornull
guinier_rg4.308
guinier_rg_error0.019
pddf_dmax18.4
pddf_dmax_errornull
porod_volume160.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first41
guinier_point_last93
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYM4_datloglog_img.png
symmetrynull
last_modified2026-03-26T13:49:38.423216+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYM4
statussuccess
started_at2026-08-11T14:27:08.487380+00:00
finished_at2026-08-11T14:27:16.242825+00:00
source_last_modified2026-03-26T13:49:38.423216+01:00
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查看完整 summary.json 原文
{
  "code": "SASDYM4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
    "publication": {
      "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
      "author_list": "Kley N, Hecht-Bucher M, Itzen A",
      "journal": "Protein Expression and Purification",
      "doi": "10.1016/j.pep.2026.106923",
      "pmid": null,
      "published_date": "2026 Mar"
    },
    "status": "released",
    "submitted_date": "2026-01-12",
    "released_date": "2026-03-26"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYM4.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYM4_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYM4_guinier_img.png",
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  "experiment": {
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        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Heat shock cognate 71 kDa protein",
          "short_name": "Hsc70",
          "sequence": "SNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD",
          "organism": null,
          "uniprot_code": "P11142",
          "uniprot_range_first": 1,
          "uniprot_range_last": 646,
          "oligomerization": "other",
          "molecular_type": "protein",
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          "molecule_source": "biological",
          "molecule_description": ""
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        "concentration_unit": null,
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        "concentration": null,
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        "ph": 7.5,
        "deuteration": null
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      "purity_method": null,
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      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
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      "mixture": null
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    "contributor": [
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        "affiliation": [
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            "address": "Hamburg, Germany",
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        "orcid": ""
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    "concentration_method": null,
    "concentration_unit": null,
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    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
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    "number_of_frames": 40,
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    "sample_detector_distance": 3.0,
    "concentration_min": null,
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    "flow_rate": null,
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  "fits": [],
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  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
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  "guinier_i0_error": null,
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  "porod_volume_error": null,
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  "guinier_point_first": 41,
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  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDYM4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-26T13:49:38.423216+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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