Phosphorylated (T495) Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (5 mg/ml)

{
  "code": "SASDYR5",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
    "publication": {
      "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
      "author_list": "Kley N, Hecht-Bucher M, Itzen A",
      "journal": "Protein Expression and Purification",
      "doi": "10.1016/j.pep.2026.106923",
      "pmid": null,
      "published_date": "2026 Mar"
    },
    "status": "released",
    "submitted_date": "2026-01-12",
    "released_date": "2026-03-26"
  },
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  "experiment": {
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      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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      "beam_profile_al": null
    },
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        {
          "long_name": "Phosphorylated Heat shock cognate 71 kDa protein",
          "short_name": "pHsc70",
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          "organism": "Homo sapiens",
          "uniprot_code": "P11142",
          "uniprot_range_first": 1,
          "uniprot_range_last": 646,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA\nMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVS\nSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAA\nIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNH\nFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRA\nRFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELN\nKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTI\nPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDI\nDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKN\nSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELE\nKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD",
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          "total_mw": 71.142,
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          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Protein 86 % phosphorylated at T495"
        }
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        "name": "20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP",
        "concentration_unit": null,
        "comment": "HEPES buffer with 1 mM ADP",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Phosphorylated (T495) Heat shock cognate 71 kDa protein (Hsc70) T204A with ADP (5 mg/ml)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Hamburg, Germany",
            "full_name": "University of Hamburg",
            "webpage": "https://www.uni-hamburg.de/"
          }
        ],
        "contributor_name": "Nicholas",
        "contributor_surname": "Kley",
        "orcid": ""
      }
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    "concentration_unit": null,
    "date": "2025-03-06",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.095,
    "number_of_frames": 40,
    "wavelength": 0.123979,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
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    "flow_rate": null,
    "s_min": 0.025,
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    "total_exposure_time": null,
    "seccolumn": null
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  "estimated_volume_method": null,
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  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "1/cm",
  "experimental_mw": 149.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 146.999,
  "porod_mw_error": null,
  "pddf_i0": 0.1161,
  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 5.47,
  "pddf_rg_error": null,
  "guinier_rg": 5.265,
  "guinier_rg_error": 0.019,
  "pddf_dmax": 22.8,
  "pddf_dmax_error": null,
  "porod_volume": 236.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 24,
  "guinier_point_last": 65,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDYR5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-26T13:53:01.618009+01:00",
  "bragg_peak": []
}

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  "finished_at": "2026-08-11T14:54:35.890389+00:00",
  "source_last_modified": "2026-03-26T13:53:01.618009+01:00",
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