SASDYT2

Full-length yeast shuttle protein Dsk2

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-02-22T17:31:24.214742+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Full-length yeast shuttle protein Dsk2

浓度— – 6.11 缓冲液 / pH20 mM NaPhos, 0.5 mM EDTA, 0.02% NaN3 / 6.8
Experimental temperature25.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.124 nm曝光2.0 s × 600

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Ubiquitin domain-containing protein DSK2
查看序列
MSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRLIYSGKILKDDQTVESYHIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPNMSSGQSAGFNPLADLTSARYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIFQSQMNEMLSNPQMLDFMIQSNPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFARMMDPNAGMGSAGGAASAFPAPGGDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAANPFASLLNPALNPFANAGNAASTGMPAFDPALLASMFQPPVQASQAEDTRPPEERYEHQLRQLNDMGFFDFDRNVAALRRSGGSVQGALDSLLNGDV
proteinSaccharomyces cerevisiaeP485101–373monomer分子数 139.346 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1912[3]0.011402–0.472902 1/A含误差列缺失 01sasbdb/entries/t2/sasdyt2/source/SASDYT2.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
i0Guinier64.3219arbitrary
mwExperimental40.0kDa
rgGuinier3.7910.073Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM NaPhos, 0.5 mM EDTA, 0.02% NaN3缓冲液浓度
pH6.8添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-11-04储存 / 测量温度25.0 / 25.0
曝光时间2.0帧数600
波长0.124样品-探测器距离2.077
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.114 – 4.729样品体积 / 流速300.0 / 0.65

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/t2/sasdyt2/source/SASDYT2.datdownloaded50284a534bd873aab155b7f464adf8fed9b8a487d2b2536c3419afd47c7d8d20d529dDownload查看原文件源站
full_entry_zipsasbdb/entries/t2/sasdyt2/source/SASDYT2.zipdownloaded15445e35c7bcf92a6d0c96dab9ae36396d3aeba1c50065af6ef81b9e46c843305cc91Download查看原文件源站
pddfsasbdb/entries/t2/sasdyt2/source/SASDYT2.outnot_listedDownload查看原文件
sascifsasbdb/entries/t2/sasdyt2/source/SASDYT2.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/t2/sasdyt2/source/summary.jsondownloaded55177fe5a7f9f3d6460d6e8afad7ec174d59b1b4d63b4433de2f4b48172bd18459cdDownload查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(1 项)
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pddf:来源记录
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  "role": "pddf",
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sascif:来源记录
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  "role": "sascif",
  "status": "not_available",
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:128 个字段值
字段路径原始值
codeSASDYT2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSTI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation.
project.publication.titleSTI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation.
project.publication.author_listAcharya N, Daniel EA, Dao TP, Niblo JK, Mulvey EO, Sukenik S, Kraut DA, Roelofs J, Castañeda CA
project.publication.journalEMBO J
project.publication.doi10.1038/s44318-026-00696-1
project.publication.pmid41673446
project.publication.published_date2026 Feb 11
project.statusreleased
project.submitted_date2025-11-24
project.released_date2025-12-22
pddf_datanull
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYT2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_kratky_img.png
pddf_plotnull
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDYT2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameUbiquitin domain-containing protein DSK2
experiment.sample.molecule[0].short_nameDSK2
experiment.sample.molecule[0].sequenceMSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRLIYSGKILKDDQTVESYHIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPNMSSGQSAGFNPLADLTSARYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIFQSQMNEMLSNPQMLDFMIQSNPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFARMMDPNAGMGSAGGAASAFPAPGGDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAANPFASLLNPALNPFANAGNAASTGMPAFDPALLASMFQPPVQASQAEDTRPPEERYEHQLRQLNDMGFFDFDRNVAALRRSGGSVQGALDSLLNGDV
experiment.sample.molecule[0].organismSaccharomyces cerevisiae
experiment.sample.molecule[0].uniprot_codeP48510
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last373
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRLIYSGKILKDDQTVESY HIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPNMSSGQSAGFNPLADLTSA RYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIFQSQMNEMLSNPQMLDFMIQS NPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFARMMDPNAGMGSAGGAASAFPAPG GDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAANPFASLLNPALNPFANAGNAASTG MPAFDPALLASMFQPPVQASQAEDTRPPEERYEHQLRQLNDMGFFDFDRNVAALRRSGGS VQGALDSLLNGDV
experiment.sample.molecule[0].mw39.346
experiment.sample.molecule[0].total_mw39.346
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM NaPhos, 0.5 mM EDTA, 0.02% NaN3
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFull-length yeast shuttle protein Dsk2
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-11-04
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time2.0
experiment.number_of_frames600
experiment.wavelength0.124
experiment.sample_detector_distance2.077
experiment.concentration_minnull
experiment.concentration_max6.11
experiment.sample_volume300.0
experiment.flow_rate0.65
experiment.s_min0.114
experiment.s_max4.729
experiment.total_exposure_timenull
experiment.seccolumn17
fits[]
estimated_volume_methodnull
pddf_softwarenull
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw40.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i064.3219
guinier_i0_errornull
pddf_rgnull
pddf_rg_errornull
guinier_rg3.791
guinier_rg_error0.073
pddf_dmaxnull
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first6
guinier_point_last31
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYT2_datloglog_img.png
symmetrynull
last_modified2026-02-22T17:31:24.214742+01:00
bragg_peak[]
manifest.json:31 个字段值
字段路径原始值
codeSASDYT2
statussuccess
started_at2026-08-11T13:46:12.574736+00:00
finished_at2026-08-11T13:46:18.486049+00:00
source_last_modified2026-02-22T17:31:24.214742+01:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDYT2/
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files[0].size5517
files[0].sha2567fe5a7f9f3d6460d6e8afad7ec174d59b1b4d63b4433de2f4b48172bd18459cd
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files[1].path/data/saxsdb/sasbdb/entries/t2/sasdyt2/source/SASDYT2.dat
files[1].size50284
files[1].sha256a534bd873aab155b7f464adf8fed9b8a487d2b2536c3419afd47c7d8d20d529d
files[1].rolecurve
files[2].rolepddf
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files[2].path/data/saxsdb/sasbdb/entries/t2/sasdyt2/source/SASDYT2.out
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files[3].path/data/saxsdb/sasbdb/entries/t2/sasdyt2/source/SASDYT2.sascif
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDYT2.zip
files[4].path/data/saxsdb/sasbdb/entries/t2/sasdyt2/source/SASDYT2.zip
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files[4].rolefull_entry_zip
files[4].zip_member_count1
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDYT2",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation.",
    "publication": {
      "title": "STI1 domain engages transient helices to mediate Dsk2 phase separation and proteasome condensation.",
      "author_list": "Acharya N, Daniel EA, Dao TP, Niblo JK, Mulvey EO, Sukenik S, Kraut DA, Roelofs J, Castañeda CA",
      "journal": "EMBO J",
      "doi": "10.1038/s44318-026-00696-1",
      "pmid": "41673446",
      "published_date": "2026 Feb 11"
    },
    "status": "released",
    "submitted_date": "2025-11-24",
    "released_date": "2025-12-22"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYT2.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYT2_guinier_img.png",
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      "country": "USA",
      "beamline_name": "12.3.1 (SIBYLS)",
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      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
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          "long_name": "Ubiquitin domain-containing protein DSK2",
          "short_name": "DSK2",
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          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "P48510",
          "uniprot_range_first": 1,
          "uniprot_range_last": 373,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRLIYSGKILKDDQTVESY\nHIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPNMSSGQSAGFNPLADLTSA\nRYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIFQSQMNEMLSNPQMLDFMIQS\nNPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFARMMDPNAGMGSAGGAASAFPAPG\nGDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAANPFASLLNPALNPFANAGNAASTG\nMPAFDPALLASMFQPPVQASQAEDTRPPEERYEHQLRQLNDMGFFDFDRNVAALRRSGGS\nVQGALDSLLNGDV",
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          "total_mw": 39.346,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": ""
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
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      "ext_coefficient": null,
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  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
  "guinier_i0": 64.3219,
  "guinier_i0_error": null,
  "pddf_rg": null,
  "pddf_rg_error": null,
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  "guinier_rg_error": 0.073,
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  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
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  "estimated_volume_error": null,
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  "guinier_point_last": 31,
  "pddf_point_first": null,
  "pddf_point_last": null,
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  "intensities_log_log_plot": "SASDYT2_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-02-22T17:31:24.214742+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDYT2",
  "status": "success",
  "started_at": "2026-08-11T13:46:12.574736+00:00",
  "finished_at": "2026-08-11T13:46:18.486049+00:00",
  "source_last_modified": "2026-02-22T17:31:24.214742+01:00",
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