SASDYV4

Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-03-26T13:49:57.877450+01:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Heat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)

浓度— – 1.0 缓冲液 / pH20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP / 7.5
Experimental temperature20.0 设备 / 束线PETRA III / EMBL P12
波长0.123979 nm曝光0.095 s × 40

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Heat shock cognate 71 kDa protein
查看序列
SNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
proteinP111421–646other分子数 171.142 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12593[3]0.002247303–0.7247599 1/A含误差列缺失 0492sasbdb/entries/v4/sasdyv4/source/SASDYV4.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)22.0Å
i0Guinier0.08742341/cm
i0P(r)0.088981/cm
mwExperimental113.0kDa
mwPorod157.0kDa
porod_volumePorod252.0ų
rgGuinier5.2390.045Å
rgP(r)5.54Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP缓冲液浓度
pH7.5添加剂
缓冲液说明HEPES buffer with 1 mM ADP
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-03-06储存 / 测量温度4.0 / 20.0
曝光时间0.095帧数40
波长0.123979样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.022 – 7.248样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/v4/sasdyv4/source/SASDYV4.datdownloaded111991e17145aeda03d8bdb9d01177880d1501bc78ae6b98f52ade940ec14eed3e9ba7Download查看原文件源站
full_entry_zipsasbdb/entries/v4/sasdyv4/source/SASDYV4.zipdownloaded49883dadae518a33a0b17bfae787d59223021eeb6c0a878fdef5dbf8bb60bdfbd224fDownload查看原文件源站
pddfsasbdb/entries/v4/sasdyv4/source/SASDYV4.outdownloaded639642b18c5add51be80bee5a684c5f2e7037d6747ed4e38541360b78f7e8424fa4b5Download查看原文件源站
sascifsasbdb/entries/v4/sasdyv4/source/SASDYV4.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/v4/sasdyv4/source/summary.jsondownloaded65308f39af58b1a0a43b4c39671f2398e60bd4956c075c2b81c340fc44856cb0e62aDownload查看原文件源站
curve:来源记录
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}
full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDYV4.zip",
  "zip_member_count": 2,
  "zip_members": [
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pddf:来源记录
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  "role": "pddf",
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sascif:来源记录
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  "role": "sascif",
  "status": "not_available",
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summary:来源记录
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  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDYV4/"
}

全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDYV4
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleAdvances on the large-scale bacterial production and stable phosphorylation of Hsc70
project.publication.titleAdvances on the large-scale bacterial production and stable phosphorylation of Hsc70
project.publication.author_listKley N, Hecht-Bucher M, Itzen A
project.publication.journalProtein Expression and Purification
project.publication.doi10.1016/j.pep.2026.106923
project.publication.pmidnull
project.publication.published_date2026 Mar
project.statusreleased
project.submitted_date2026-01-12
project.released_date2026-03-26
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDYV4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYV4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYV4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDYV4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHeat shock cognate 71 kDa protein
experiment.sample.molecule[0].short_nameHsc70
experiment.sample.molecule[0].sequenceSNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeP11142
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last646
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA MNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVS SMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAA IAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNH FIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRA RFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELN KSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTI PTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDI DANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKN SLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELE KVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD
experiment.sample.molecule[0].mw71.142
experiment.sample.molecule[0].total_mw71.142
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM HEPES, 150 mM KCl, 10 mM MgCl2, 1mM TCEP, 1mM ADP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentHEPES buffer with 1 mM ADP
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHeat shock cognate 71 kDa protein (Hsc70) T204A mutant with ADP (1 mg/ml)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressHamburg, Germany
experiment.contributor[0].affiliation[0].full_nameUniversity of Hamburg
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-hamburg.de/
experiment.contributor[0].contributor_nameNicholas
experiment.contributor[0].contributor_surnameKley
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-03-06
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time0.095
experiment.number_of_frames40
experiment.wavelength0.123979
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max1.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.022
experiment.s_max7.248
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw113.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw157.0
porod_mw_errornull
pddf_i00.08898
pddf_i0_errornull
guinier_i00.0874234
guinier_i0_errornull
pddf_rg5.54
pddf_rg_errornull
guinier_rg5.239
guinier_rg_error0.045
pddf_dmax22.0
pddf_dmax_errornull
porod_volume252.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first17
guinier_point_last80
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYV4_datloglog_img.png
symmetrynull
last_modified2026-03-26T13:49:57.877450+01:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYV4
statussuccess
started_at2026-08-11T14:32:41.970156+00:00
finished_at2026-08-11T14:32:49.954215+00:00
source_last_modified2026-03-26T13:49:57.877450+01:00
files[0].statusdownloaded
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files[0].size6530
files[0].sha2568f39af58b1a0a43b4c39671f2398e60bd4956c075c2b81c340fc44856cb0e62a
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files[1].size111991
files[1].sha256e17145aeda03d8bdb9d01177880d1501bc78ae6b98f52ade940ec14eed3e9ba7
files[1].rolecurve
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDYV4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
    "publication": {
      "title": "Advances on the large-scale bacterial production and stable phosphorylation of Hsc70",
      "author_list": "Kley N, Hecht-Bucher M, Itzen A",
      "journal": "Protein Expression and Purification",
      "doi": "10.1016/j.pep.2026.106923",
      "pmid": null,
      "published_date": "2026 Mar"
    },
    "status": "released",
    "submitted_date": "2026-01-12",
    "released_date": "2026-03-26"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYV4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYV4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYV4_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDYV4_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYV4.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Heat shock cognate 71 kDa protein",
          "short_name": "Hsc70",
          "sequence": "SNAMSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGAFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD",
          "organism": null,
          "uniprot_code": "P11142",
          "uniprot_range_first": 1,
          "uniprot_range_last": 646,
          "oligomerization": "other",
          "molecular_type": "protein",
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    "concentration_unit": null,
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  "pddf_software_version": null,
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  "description": null,
  "experiment_description": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDYV4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-03-26T13:49:57.877450+01:00",
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}
查看完整 manifest.json 原文
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