SASDYV7

Chimeric constructs - CC_2 (Jo_AtCel8A:In-AtCel9R)

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2026-06-18T15:58:40.527242+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Chimeric constructs - CC_2 (Jo_AtCel8A:In-AtCel9R)

Concentration1.7 – 20.0 Buffer / pH25 mM Tris, 150 mM NaCl / 8.0
Experimental temperature20.0 Facility / beamlineLaboratoire de Génie Chimique / XEUSS 2.0
Wavelength0.154 nmExposure600.0 s × 12

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Jo_AtCel8A:In-AtCel9R
View sequence
MGSSRYSDQYPQTGTYPDVQTPYQIIKVDGSEKNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDGYGVPFNTKYPYGPTSIADNQSEVTAMLKAEWEDWKSKRITSNGAGGYKRVQRDASTNYDTVSEGMGYGLLLAVCFNEQALFDDLYRYVKSHFNGNGLMHWHIDANNNVTSHDGGDGAATDADEDIALALIFADKLWGSSGAINYGQEARTLINNLYNHCVEHGSYVLKPGDRWGGSSVTNPSYFAPAWYKVYAQYTGDTRWNQVADKCYQIVEEVKKYNNGTGLVPDWCTASGTPASGQSYDYKYDATRYGWRTAVDYSWFGDQRAKANCDMLTKFFARDGAKGIVDGYTIQGSKISNNHNASFIGPVAAASMTGYDLNFAKELYRETVAVKDSEYYGYYGNSLRLLTLLYITGNFPNPLSDLSGQASHHHHHHMGSSRYTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRLENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSNKFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDGYADYNYGEALQKAIMFYEFQMSGKLPDNIRNNWRGDSCLGDGSDVGLDLTGGWFDAGDHVKFNLPMAYTATMLAWAVYEYKDALQKSGQLGYLMDQIKWASDYFIRCHPEKYVYYYQVGNGDMDHRWWVPAECIDVQAPRPSYKVDLSNPGSTVTAGTAAALAATALVFKDTDPAYAALCIRHAKELFDFAETTMSDKGYTAALNFYTSHSGWYDELSWAGAWIYLADGDETYLEKAEKYVDKWPIESQTTYIAYSWGHCWDDVHYGAALLLAKITNKSLYKEAIERHLDYWTVGFNGQRVRYTPKGLAHLTDWGVLRHATTTAFLACVYSDWSECPREKANIYIDFAKKQADYALGSSGRSYVVGFGVNPPQHPHHRTAHSSWCDSQKVPEYHRHVLYGALVGGPDASDAYVDDIGNYVTNEVACDYNAGFVGLLAKMYEKYGGNPIPNFMAIEEKTNEEIYVEATANSNNGVELKTYLYNKSGWPARVCDKLSFRYFMDLTEYVSAGYNPNDITVSIIYSAAPTAKISKPILYDASKNIYYCEIDLSGTKIFPGSNSDHQKETQFRIQPPAGAPWDNTNDFSYQGIKKNGEVVKEMPVYEDGVLIFGVEPNGTGAS
proteinAcetovibrio thermocellus / Streptococcus pneumoniae——–—monomerMolecule count 1136.311 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1861[3]0.009972–0.500031 1/AHas uncertainty columnMissing 00sasbdb/entries/v7/sasdyv7/source/SASDYV7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.36—Å
i0Guinier0.0122852—arbitrary
i0P(r)0.01213—arbitrary
mwExperimental136.8—kDa
mwPorod142.4—kDa
porod_volumePorod172.0—ų
rgGuinier4.3180.238Å
rgP(r)4.22—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris, 150 mM NaClBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-04-04Storage / measurement temperature4.0 / 20.0
Exposure time600.0Frames12
Wavelength0.154Sample-to-detector distance1.26
SourceX-ray in houseDetectorPilatus 1M detector (DECTRIS, Switzerland)
Facility / beamlineLaboratoire de Génie Chimique / XEUSS 2.0 · Toulouse, France
q range0.1 – 5.0Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDYV7
statusPublished
type_of_curveMerged
angular_unit1/A
project.titleIntegrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.
project.publication.titleIntegrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.
project.publication.author_listPardo Larrabeiti I, Eibinger M, Esque J, Pradeau S, Fort S, Moraïs S, Mizrahi I, Bayer EA, Nidetzky B, Montanier CY, Roblin P, Dumon C
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.70649
project.publication.pmid42298785
project.publication.published_date2026 Jul
project.statusreleased
project.submitted_date2026-02-27
project.released_date2026-06-18
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDYV7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDYV7.dat
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 1M detector (DECTRIS, Switzerland)
experiment.instrument.detector.resolution172.0
experiment.instrument.nameLaboratoire de Génie Chimique
experiment.instrument.cityToulouse
experiment.instrument.countryFrance
experiment.instrument.beamline_nameXEUSS 2.0
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameJo_AtCel8A:In-AtCel9R
experiment.sample.molecule[0].short_nameCC_2
experiment.sample.molecule[0].sequenceMGSSRYSDQYPQTGTYPDVQTPYQIIKVDGSEKNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDGYGVPFNTKYPYGPTSIADNQSEVTAMLKAEWEDWKSKRITSNGAGGYKRVQRDASTNYDTVSEGMGYGLLLAVCFNEQALFDDLYRYVKSHFNGNGLMHWHIDANNNVTSHDGGDGAATDADEDIALALIFADKLWGSSGAINYGQEARTLINNLYNHCVEHGSYVLKPGDRWGGSSVTNPSYFAPAWYKVYAQYTGDTRWNQVADKCYQIVEEVKKYNNGTGLVPDWCTASGTPASGQSYDYKYDATRYGWRTAVDYSWFGDQRAKANCDMLTKFFARDGAKGIVDGYTIQGSKISNNHNASFIGPVAAASMTGYDLNFAKELYRETVAVKDSEYYGYYGNSLRLLTLLYITGNFPNPLSDLSGQASHHHHHHMGSSRYTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRLENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSNKFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDGYADYNYGEALQKAIMFYEFQMSGKLPDNIRNNWRGDSCLGDGSDVGLDLTGGWFDAGDHVKFNLPMAYTATMLAWAVYEYKDALQKSGQLGYLMDQIKWASDYFIRCHPEKYVYYYQVGNGDMDHRWWVPAECIDVQAPRPSYKVDLSNPGSTVTAGTAAALAATALVFKDTDPAYAALCIRHAKELFDFAETTMSDKGYTAALNFYTSHSGWYDELSWAGAWIYLADGDETYLEKAEKYVDKWPIESQTTYIAYSWGHCWDDVHYGAALLLAKITNKSLYKEAIERHLDYWTVGFNGQRVRYTPKGLAHLTDWGVLRHATTTAFLACVYSDWSECPREKANIYIDFAKKQADYALGSSGRSYVVGFGVNPPQHPHHRTAHSSWCDSQKVPEYHRHVLYGALVGGPDASDAYVDDIGNYVTNEVACDYNAGFVGLLAKMYEKYGGNPIPNFMAIEEKTNEEIYVEATANSNNGVELKTYLYNKSGWPARVCDKLSFRYFMDLTEYVSAGYNPNDITVSIIYSAAPTAKISKPILYDASKNIYYCEIDLSGTKIFPGSNSDHQKETQFRIQPPAGAPWDNTNDFSYQGIKKNGEVVKEMPVYEDGVLIFGVEPNGTGAS
experiment.sample.molecule[0].organismAcetovibrio thermocellus / Streptococcus pneumoniae
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw136.311
experiment.sample.molecule[0].total_mw136.311
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name25 mM Tris, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameChimeric constructs - CC_2 (Jo_AtCel8A:In-AtCel9R)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameToulouse Biotechnology Institute
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameIker
experiment.contributor[0].contributor_surnamePARDO
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-04-04
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time600.0
experiment.number_of_frames12
experiment.wavelength0.154
experiment.sample_detector_distance1.26
experiment.concentration_min1.7
experiment.concentration_max20.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.1
experiment.s_max5.0
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw136.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw142.4
porod_mw_errornull
pddf_i00.01213
pddf_i0_errornull
guinier_i00.0122852
guinier_i0_errornull
pddf_rg4.22
pddf_rg_errornull
guinier_rg4.318
guinier_rg_error0.238
pddf_dmax13.36
pddf_dmax_errornull
porod_volume172.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last35
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYV7_datloglog_img.png
symmetrynull
last_modified2026-06-18T15:58:40.527242+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDYV7
statussuccess
started_at2026-08-11T15:41:08.537219+00:00
finished_at2026-08-11T15:41:16.285899+00:00
source_last_modified2026-06-18T15:58:40.527242+02:00
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View the complete summary.json source
{
  "code": "SASDYV7",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/A",
  "project": {
    "title": "Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.",
    "publication": {
      "title": "Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization.",
      "author_list": "Pardo Larrabeiti I, Eibinger M, Esque J, Pradeau S, Fort S, Moraïs S, Mizrahi I, Bayer EA, Nidetzky B, Montanier CY, Roblin P, Dumon C",
      "journal": "Protein Sci",
      "doi": "10.1002/pro.70649",
      "pmid": "42298785",
      "published_date": "2026 Jul"
    },
    "status": "released",
    "submitted_date": "2026-02-27",
    "released_date": "2026-06-18"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDYV7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYV7.dat",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDYV7_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDYV7.sascif",
  "experiment": {
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      "detector": {
        "type": null,
        "name": "Pilatus 1M detector (DECTRIS, Switzerland)",
        "resolution": 172.0
      },
      "name": "Laboratoire de Génie Chimique",
      "city": "Toulouse",
      "country": "France",
      "beamline_name": "XEUSS 2.0",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "Jo_AtCel8A:In-AtCel9R",
          "short_name": "CC_2",
          "sequence": "MGSSRYSDQYPQTGTYPDVQTPYQIIKVDGSEKNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDGYGVPFNTKYPYGPTSIADNQSEVTAMLKAEWEDWKSKRITSNGAGGYKRVQRDASTNYDTVSEGMGYGLLLAVCFNEQALFDDLYRYVKSHFNGNGLMHWHIDANNNVTSHDGGDGAATDADEDIALALIFADKLWGSSGAINYGQEARTLINNLYNHCVEHGSYVLKPGDRWGGSSVTNPSYFAPAWYKVYAQYTGDTRWNQVADKCYQIVEEVKKYNNGTGLVPDWCTASGTPASGQSYDYKYDATRYGWRTAVDYSWFGDQRAKANCDMLTKFFARDGAKGIVDGYTIQGSKISNNHNASFIGPVAAASMTGYDLNFAKELYRETVAVKDSEYYGYYGNSLRLLTLLYITGNFPNPLSDLSGQASHHHHHHMGSSRYTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRLENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSNKFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDGYADYNYGEALQKAIMFYEFQMSGKLPDNIRNNWRGDSCLGDGSDVGLDLTGGWFDAGDHVKFNLPMAYTATMLAWAVYEYKDALQKSGQLGYLMDQIKWASDYFIRCHPEKYVYYYQVGNGDMDHRWWVPAECIDVQAPRPSYKVDLSNPGSTVTAGTAAALAATALVFKDTDPAYAALCIRHAKELFDFAETTMSDKGYTAALNFYTSHSGWYDELSWAGAWIYLADGDETYLEKAEKYVDKWPIESQTTYIAYSWGHCWDDVHYGAALLLAKITNKSLYKEAIERHLDYWTVGFNGQRVRYTPKGLAHLTDWGVLRHATTTAFLACVYSDWSECPREKANIYIDFAKKQADYALGSSGRSYVVGFGVNPPQHPHHRTAHSSWCDSQKVPEYHRHVLYGALVGGPDASDAYVDDIGNYVTNEVACDYNAGFVGLLAKMYEKYGGNPIPNFMAIEEKTNEEIYVEATANSNNGVELKTYLYNKSGWPARVCDKLSFRYFMDLTEYVSAGYNPNDITVSIIYSAAPTAKISKPILYDASKNIYYCEIDLSGTKIFPGSNSDHQKETQFRIQPPAGAPWDNTNDFSYQGIKKNGEVVKEMPVYEDGVLIFGVEPNGTGAS",
          "organism": "Acetovibrio thermocellus / Streptococcus pneumoniae",
          "uniprot_code": null,
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