SASDYW3

Point mutant M65I of EVH1 domain of Homer protein homolog 1 from mouse monomer

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2026-06-01T15:03:22.566079+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Point mutant M65I of EVH1 domain of Homer protein homolog 1 from mouse monomer

浓度— – 4.0 缓冲液 / pH20mM NaCl, 50 mM NaPi, 0.02% of sodium-azide / 7.4
Experimental temperature25.0 设备 / 束线CEITEC / Rigaku BioSAXS-2000
波长1.54 nm曝光600.0 s × 10

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Point mutant M65I of EVH1 domain of Homer protein homolog 1 from mouse monomer
查看序列
GSHMGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNITFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQ
proteinMus musculusQ9Z2Y33–121monomer分子数 113.7 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1777[3]0.00341288–0.644208 1/A含误差列缺失 027sasbdb/entries/w3/sasdyw3/source/SASDYW3.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)5.25Å
i0Guinier0.722068arbitrary
i0P(r)0.7129arbitrary
mwExperimental12.0kDa
mwPorod14.88kDa
porod_volumePorod23.88ų
rgGuinier1.6840.122Å
rgP(r)1.65Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20mM NaCl, 50 mM NaPi, 0.02% of sodium-azide缓冲液浓度
pH7.4添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-11-08储存 / 测量温度4.0 / 25.0
曝光时间600.0帧数10
波长1.54样品-探测器距离0.48
光源X-ray in house探测器Rigaku HyPix-3000
机构 / 束线CEITEC / Rigaku BioSAXS-2000 · Brno, Czech Republic
q range0.034 – 6.442样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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sascifsasbdb/entries/w3/sasdyw3/source/SASDYW3.sascifnot_availableDownload查看原文件源站
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDYW3
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleModulation of Homer1 EVH1 domain internal dynamics by putative autism-associated mutations.
project.publication.titleModulation of Homer1 EVH1 domain internal dynamics by putative autism-associated mutations.
project.publication.author_listFarkas F, Maruzs B, Kálmán ZE, Klumpler T, Batta G, Péterfia B, Gáspári Z
project.publication.journalFEBS Lett
project.publication.doi10.1002/1873-3468.70369
project.publication.pmid42206738
project.publication.published_date2026 May 28
project.statusreleased
project.submitted_date2025-12-10
project.released_date2026-06-01
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experiment.instrument.detector.resolution100.0
experiment.instrument.nameCEITEC
experiment.instrument.cityBrno
experiment.instrument.countryCzech Republic
experiment.instrument.beamline_nameRigaku BioSAXS-2000
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experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
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experiment.sample.molecule[0].long_namePoint mutant M65I of EVH1 domain of Homer protein homolog 1 from mouse monomer
experiment.sample.molecule[0].short_nameEVH1 Homer M65I
experiment.sample.molecule[0].sequenceGSHMGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNITFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQ
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeQ9Z2Y3
experiment.sample.molecule[0].uniprot_range_first3
experiment.sample.molecule[0].uniprot_range_last121
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw13.7
experiment.sample.molecule[0].total_mw13.7
experiment.sample.molecule[0].number_molecules1
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experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcesynthetic
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20mM NaCl, 50 mM NaPi, 0.02% of sodium-azide
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePoint mutant M65I of EVH1 domain of Homer protein homolog 1 from mouse monomer
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experiment.sample.contrastnull
experiment.sample.specific_volnull
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experiment.contributor[0].affiliation[0].webpagehttps://www.ceitec.eu/
experiment.contributor[0].contributor_nameTomas
experiment.contributor[0].contributor_surnameKlumpler
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experiment.concentration_unitnull
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experiment.cell_temperature25.0
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descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw12.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw14.88
porod_mw_errornull
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estimated_volume_errornull
guinier_point_first14
guinier_point_last90
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symmetrynull
last_modified2026-06-01T15:03:22.566079+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYW3
statussuccess
started_at2026-08-11T14:09:54.404797+00:00
finished_at2026-08-11T14:10:02.609826+00:00
source_last_modified2026-06-01T15:03:22.566079+02:00
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查看完整 summary.json 原文
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Modulation of Homer1 EVH1 domain internal dynamics by putative autism-associated mutations.",
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      "title": "Modulation of Homer1 EVH1 domain internal dynamics by putative autism-associated mutations.",
      "author_list": "Farkas F, Maruzs B, Kálmán ZE, Klumpler T, Batta G, Péterfia B, Gáspári Z",
      "journal": "FEBS Lett",
      "doi": "10.1002/1873-3468.70369",
      "pmid": "42206738",
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查看完整 manifest.json 原文
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