SASDYZ6

trimeric tailspike protein depolymerase Dpo31, mature form without D4

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-06-20T16:07:22.968337+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · trimeric tailspike protein depolymerase Dpo31, mature form without D4

浓度— – 23.6 缓冲液 / pHTris-HCl 50 mM pH 8, NaCl 100 mM / 8.0
Experimental temperature15.0 设备 / 束线SOLEIL / SWING
波长0.1033 nm曝光0.5 s × 250

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
tailspike protein depolymerase Dpo31
查看序列
ATTVFPYAFRIFESSDLEVYLTDEDGDQALLIEGTDYTVSGAGDEEGGEITFPVSGDPLDDGETLTILRVIDITQETDLKNQGAYYPEVVEDEFDRSRMIDQQQQEQLDRALIKTETGDRWEGQGVPAKNFAMSDPVEDTDLPTVRWTKDYVTQMAEGITGDIGAYTVVAPTSGDEKRLDEWMDDIQRPDDSLVVADGGTEARSLSERFADSASYQDYGIAGDGTTNDTAAFAALESDRSSDAIELHGNTYLVDEIPNGNAYRDAVWSLDGEDLSISEYGGLVTGTPTTGAFEPAYTGGVNNTPTTSGRTNKHTRAILASQNCRADFARSACVASIYSWAYGNVSGNFASRQSIAGAPQTVNIGSEEGQALGFQSGNYTTQFCRAEGSTTFNIGSDDCAASGAHSGTISSLESYAGRGHDFRGTPVFDDGVLVDITIDDAGAGYVPGSDVMYLQNRQFGNTTDAVITYTVDGTGGVSAITITDGGSGYSGIVAARIDTFGDYSLVMASARSKIEDQFCAAIASDNARVRGRESAVIASDGGVVNEDNSVVIGSVDSTSNGARSGIYTGSGCETTGAGAVVIGGVNAKASNDGAIVMGRGVDSEFARSLVFGDGGSGAAASTAGRKFQVTAAGNVTAAGTITGSTTYA
proteinCobetia phage Carin117–665trimer分子数 167.5 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11149[3]0.0031927–0.5253204 1/A含误差列缺失 02sasbdb/entries/z6/sasdyz6/source/SASDYZ6.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)28.59Å
i0Guinier0.167443arbitrary
i0P(r)0.1707arbitrary
mwExperimental185.8kDa
mwGuinier I(0)213.0kDa
mwPorod217.0kDa
porod_volumePorod307.0ų
rgGuinier6.990.411Å
rgP(r)7.58Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称Tris-HCl 50 mM pH 8, NaCl 100 mM缓冲液浓度
pH8.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2019-07-10储存 / 测量温度4.0 / 15.0
曝光时间0.5帧数250
波长0.1033样品-探测器距离1.807
光源X-ray synchrotron探测器AVIEX PCCD170170
机构 / 束线SOLEIL / SWING · Saint-Aubin, France
q range0.032 – 5.253样品体积 / 流速70.0 / 0.2

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/z6/sasdyz6/source/SASDYZ6.outdownloaded8038654e4c72b366b3ce24e2d8fe68b434b552cd69e36db62b11c58e9e2d4a6b2aee2Download查看原文件源站
sascifsasbdb/entries/z6/sasdyz6/source/SASDYZ6.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/z6/sasdyz6/source/summary.jsondownloaded73046651784c36e6d74da83727ac74d57a98f43271384e568975b03f4835a091e433Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDYZ6
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleBiochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.
project.publication.titleBiochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.
project.publication.author_listSirigu S, Roret T, Mocaër PY, Larocque R, Jouanneau D, Legrand P, Baudoux AC, Czjzek M
project.publication.journalActa Crystallogr D Struct Biol
project.publication.doi10.1107/S2059798326005425
project.publication.pmid42308020
project.publication.published_date2026 Jul 1
project.statusreleased
project.submitted_date2026-02-14
project.released_date2026-06-20
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experiment.instrument.detector.nameAVIEX PCCD170170
experiment.instrument.detector.resolutionnull
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nametailspike protein depolymerase Dpo31
experiment.sample.molecule[0].short_nameDpo31
experiment.sample.molecule[0].sequenceATTVFPYAFRIFESSDLEVYLTDEDGDQ ALLIEGTDYTVSGAGDEEGGEITFPVSGDPLDDGETLTILRVIDITQETDLKNQGAYY PEVVEDEFDRSRMIDQQQQEQLDRALIKTETGDRWEGQGVPAKNFAMSDPVEDTDLPT VRWTKDYVTQMAEGITGDIGAYTVVAPTSGDEKRLDEWMDDIQRPDDSLVVADGGTEA RSLSERFADSASYQDYGIAGDGTTNDTAAFAALESDRSSDAIELHGNTYLVDEIPNGN AYRDAVWSLDGEDLSISEYGGLVTGTPTTGAFEPAYTGGVNNTPTTSGRTNKHTRAIL ASQNCRADFARSACVASIYSWAYGNVSGNFASRQSIAGAPQTVNIGSEEGQALGFQSG NYTTQFCRAEGSTTFNIGSDDCAASGAHSGTISSLESYAGRGHDFRGTPVFDDGVLVD ITIDDAGAGYVPGSDVMYLQNRQFGNTTDAVITYTVDGTGGVSAITITDGGSGYSGIV AARIDTFGDYSLVMASARSKIEDQFCAAIASDNARVRGRESAVIASDGGVVNEDNSVV IGSVDSTSNGARSGIYTGSGCETTGAGAVVIGGVNAKASNDGAIVMGRGVDSEFARSL VFGDGGSGAAASTAGRKFQVTAAGNVTAAGTITGSTTYA
experiment.sample.molecule[0].organismCobetia phage Carin1
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_first17
experiment.sample.molecule[0].uniprot_range_last665
experiment.sample.molecule[0].oligomerizationtrimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw67.5
experiment.sample.molecule[0].total_mw202.5
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptiontrimeric tailspike protein that contains a depolymerase from the marine Cobetia phage Carin1, mature form, Dpo31
experiment.sample.buffer.nameTris-HCl 50 mM pH 8, NaCl 100 mM
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nametrimeric tailspike protein depolymerase Dpo31, mature form without D4
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameLBI2M, Sorbonne Université CNRS, UMR8227
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameMirjam
experiment.contributor[0].contributor_surnameCzjzek
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-7483-2841
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-07-10
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time0.5
experiment.number_of_frames250
experiment.wavelength0.1033
experiment.sample_detector_distance1.807
experiment.concentration_minnull
experiment.concentration_max23.6
experiment.sample_volume70.0
experiment.flow_rate0.2
experiment.s_min0.032
experiment.s_max5.253
experiment.total_exposure_timenull
experiment.seccolumn23
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fits[0].chi_square_value4.913
fits[0].p_value0.0
fits[0].fit_residual_plotSASDYZ6_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw185.8
experimental_mw_errornull
guinier_i0_mw213.0
guinier_i0_mw_errornull
porod_mw217.0
porod_mw_errornull
pddf_i00.1707
pddf_i0_errornull
guinier_i00.167443
guinier_i0_errornull
pddf_rg7.58
pddf_rg_errornull
guinier_rg6.99
guinier_rg_error0.411
pddf_dmax28.59
pddf_dmax_errornull
porod_volume307.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first9
guinier_point_last34
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDYZ6_datloglog_img.png
symmetrynull
last_modified2026-06-20T16:07:22.968337+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDYZ6
statussuccess
started_at2026-08-11T15:19:56.138115+00:00
finished_at2026-08-11T15:20:04.005205+00:00
source_last_modified2026-06-20T16:07:22.968337+02:00
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查看完整 summary.json 原文
{
  "code": "SASDYZ6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Biochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.",
    "publication": {
      "title": "Biochemical and structural characterization of a tail-spike protein with depolymerase activity identified in a marine podovirus.",
      "author_list": "Sirigu S, Roret T, Mocaër PY, Larocque R, Jouanneau D, Legrand P, Baudoux AC, Czjzek M",
      "journal": "Acta Crystallogr D Struct Biol",
      "doi": "10.1107/S2059798326005425",
      "pmid": "42308020",
      "published_date": "2026 Jul 1"
    },
    "status": "released",
    "submitted_date": "2026-02-14",
    "released_date": "2026-06-20"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDYZ6.dat",
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      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
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    "sample": {
      "molecule": [
        {
          "long_name": "tailspike protein depolymerase Dpo31",
          "short_name": "Dpo31",
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          "organism": "Cobetia phage Carin1",
          "uniprot_code": null,
          "uniprot_range_first": 17,
          "uniprot_range_last": 665,
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查看完整 manifest.json 原文
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