SASDZ46

Engineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-05-21T17:16:41.021532+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Engineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 150 mM NaCl, pH 7.0 / 7.0
Experimental temperature25.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.5 s × 1800

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Fc-hel8-L1-3
查看序列
EPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKAREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKA
proteinS.aureus—–—dimer分子数 254.441 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1411[3]0.07303728–1.224512 1/nm含误差列缺失 00sasbdb/entries/46/sasdz46/source/SASDZ46.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)30.0nm
i0Guinier3651.6arbitrary
i0P(r)3787.937arbitrary
mwExperimental120.0kDa
rgGuinier6.40.1nm
rgP(r)7.246nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 150 mM NaCl, pH 7.0缓冲液浓度
pH7.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2025-04-06储存 / 测量温度4.0 / 25.0
曝光时间0.5帧数1800
波长0.124样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.073 – 1.225样品体积 / 流速25.0 / 0.35

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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full_entry_zipsasbdb/entries/46/sasdz46/source/SASDZ46.zipdownloaded77846feedc68262c8b9558991f07c79dfb0415a41358d33cca201606e1a041ba20edDownload查看原文件源站
pddfsasbdb/entries/46/sasdz46/source/SASDZ46.outdownloaded6286f0695c92b8b9ba78dff28eb09d2cfc15999cdaf1d2d43c945552c660cd62bec6Download查看原文件源站
sascifsasbdb/entries/46/sasdz46/source/SASDZ46.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/46/sasdz46/source/summary.jsondownloaded53303d5b04bdabe8b5e446e3077c6725642e92d552174bfc46fc880919cdaa50d03cDownload查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:129 个字段值
字段路径原始值
codeSASDZ46
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleSEC-SAXS data of Fc-hel8-L1-3
project.publicationnull
project.statusreleased
project.submitted_date2026-04-24
project.released_date2026-05-11
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDZ46.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDZ46.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ46_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDZ46.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFc-hel8-L1-3
experiment.sample.molecule[0].short_nameFc-hel8-L1-3
experiment.sample.molecule[0].sequenceEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKAREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKA
experiment.sample.molecule[0].organismS.aureus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw54.441
experiment.sample.molecule[0].total_mw108.882
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionEngineered LysM-CHAP derivative of autolysin LytN, fused at it's N-terminus to human IgG1 Fc and a helical linker
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, pH 7.0
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEngineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameBioNTech R&D Austria GmbH
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameAdriana
experiment.contributor[0].contributor_surnameBadarau
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-04-06
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames1800
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume25.0
experiment.flow_rate0.35
experiment.s_min0.073
experiment.s_max1.225
experiment.total_exposure_timenull
experiment.seccolumn49
fits[]
estimated_volume_methodnull
pddf_softwareOther
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i03787.937
pddf_i0_errornull
guinier_i03651.6
guinier_i0_errornull
pddf_rg7.246
pddf_rg_errornull
guinier_rg6.4
guinier_rg_error0.1
pddf_dmax30.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last46
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZ46_datloglog_img.png
symmetrynull
last_modified2026-05-21T17:16:41.021532+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDZ46
statussuccess
started_at2026-08-11T15:01:47.352173+00:00
finished_at2026-08-11T15:01:53.795534+00:00
source_last_modified2026-05-21T17:16:41.021532+02:00
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files[3].rolesascif
files[4].statusdownloaded
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDZ46",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "SEC-SAXS data of Fc-hel8-L1-3",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-04-24",
    "released_date": "2026-05-11"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDZ46.dat",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDZ46.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ46_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ46_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDZ46.sascif",
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        "resolution": null
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      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "xray_energy": null,
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          "short_name": "Fc-hel8-L1-3",
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          "uniprot_range_first": null,
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          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
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    "total_exposure_time": null,
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  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "Other",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 120.0,
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  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 3787.937,
  "pddf_i0_error": null,
  "guinier_i0": 3651.6,
  "guinier_i0_error": null,
  "pddf_rg": 7.246,
  "pddf_rg_error": null,
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  "guinier_rg_error": 0.1,
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  "estimated_volume_error": null,
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  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDZ46_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-05-21T17:16:41.021532+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDZ46",
  "status": "success",
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