SASDZ46

Engineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-05-21T17:16:41.021532+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Engineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)

Concentration— – 5.0 Buffer / pH20 mM HEPES, 150 mM NaCl, pH 7.0 / 7.0
Experimental temperature25.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.5 s × 1800

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Fc-hel8-L1-3
View sequence
EPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKAREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKA
proteinS.aureus——–—dimerMolecule count 254.441 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1411[3]0.07303728–1.224512 1/nmHas uncertainty columnMissing 00sasbdb/entries/46/sasdz46/source/SASDZ46.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)30.0—nm
i0Guinier3651.6—arbitrary
i0P(r)3787.937—arbitrary
mwExperimental120.0—kDa
rgGuinier6.40.1nm
rgP(r)7.246—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 150 mM NaCl, pH 7.0Buffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-04-06Storage / measurement temperature4.0 / 25.0
Exposure time0.5Frames1800
Wavelength0.124Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 6M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.073 – 1.225Sample volume / flow rate25.0 / 0.35

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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sascifsasbdb/entries/46/sasdz46/source/SASDZ46.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/46/sasdz46/source/summary.jsondownloaded53303d5b04bdabe8b5e446e3077c6725642e92d552174bfc46fc880919cdaa50d03cDownloadView source fileSource site
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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:129 field values
Field pathRaw value
codeSASDZ46
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleSEC-SAXS data of Fc-hel8-L1-3
project.publicationnull
project.statusreleased
project.submitted_date2026-04-24
project.released_date2026-05-11
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experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFc-hel8-L1-3
experiment.sample.molecule[0].short_nameFc-hel8-L1-3
experiment.sample.molecule[0].sequenceEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKAREAPKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKA
experiment.sample.molecule[0].organismS.aureus
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw54.441
experiment.sample.molecule[0].total_mw108.882
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionEngineered LysM-CHAP derivative of autolysin LytN, fused at it's N-terminus to human IgG1 Fc and a helical linker
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, pH 7.0
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEngineered LysM-CHAP derivative of autolysin LytN (Fc-hel8-L1-3)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameBioNTech R&D Austria GmbH
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameAdriana
experiment.contributor[0].contributor_surnameBadarau
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-04-06
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
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experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
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experiment.flow_rate0.35
experiment.s_min0.073
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experiment.total_exposure_timenull
experiment.seccolumn49
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i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i03787.937
pddf_i0_errornull
guinier_i03651.6
guinier_i0_errornull
pddf_rg7.246
pddf_rg_errornull
guinier_rg6.4
guinier_rg_error0.1
pddf_dmax30.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
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estimated_volume_errornull
guinier_point_first3
guinier_point_last46
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZ46_datloglog_img.png
symmetrynull
last_modified2026-05-21T17:16:41.021532+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDZ46
statussuccess
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View the complete summary.json source
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  "status": "Published",
  "type_of_curve": "SEC-SAS",
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    "title": "SEC-SAXS data of Fc-hel8-L1-3",
    "publication": null,
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    "submitted_date": "2026-04-24",
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View the complete manifest.json source
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