Synthetic fusion protein comprising human IgG1 Fc, the CHAP domain of engineered Staphylococcus aureus LytN, and engineered Staphylococcus simulans lysostaphin (Fc-L2a-CHAP(alt)-LSN(HEK))

{
  "code": "SASDZ88",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "SEC-SAXS data for Fc-CHAP-LSN",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-05-13",
    "released_date": "2026-07-23"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDZ88.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZ88_dat_img.png",
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZ88_pofr_img.png",
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 6M",
        "resolution": null
      },
      "name": "PETRA III",
      "city": "DESY; Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL P12",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "Fc-L2a-CHAP(alt)-LSN(HEK)",
          "short_name": "L2184",
          "sequence": "GPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKEAAAKAKPETVSGNEKSNSGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGYYAKDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGNLQKFQSLDQNWNGGGERKQEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK",
          "organism": "synthetic construct",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
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          "total_mw": 155.948,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Human IgG1 Fc fused via a helical linker to a genetic fusion of the CHAP domain of an engineered derivative  of S.aureus LytN and an engineered derivative of S.simulans lysostaphin."
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 150 mM NaCl, pH 7.0",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Synthetic fusion protein comprising human IgG1 Fc, the CHAP domain of engineered Staphylococcus aureus LytN, and engineered Staphylococcus simulans lysostaphin (Fc-L2a-CHAP(alt)-LSN(HEK))",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "BioNTech R&D Austria GmbH",
            "webpage": null
          }
        ],
        "contributor_name": "Adriana",
        "contributor_surname": "Badarau",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-04-06",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": 0.5,
    "number_of_frames": 1800,
    "wavelength": 0.124,
    "sample_detector_distance": 3.0,
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    "concentration_max": 5.0,
    "sample_volume": 25.0,
    "flow_rate": 0.35,
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    "s_max": 1.283,
    "total_exposure_time": null,
    "seccolumn": 49
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  "estimated_volume_method": null,
  "pddf_software": "Other",
  "pddf_software_version": "GNOM",
  "i0_calibration_standard": null,
  "description": null,
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  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
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  "pddf_rg": 9.963,
  "pddf_rg_error": null,
  "guinier_rg": 8.9,
  "guinier_rg_error": 0.2,
  "pddf_dmax": 40.0,
  "pddf_dmax_error": null,
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  "guinier_point_first": 12,
  "guinier_point_last": 45,
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  "symmetry": null,
  "last_modified": "2026-07-23T17:37:52.487403+02:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T15:48:09.870462+00:00",
  "finished_at": "2026-08-11T15:48:16.387152+00:00",
  "source_last_modified": "2026-07-23T17:37:52.487403+02:00",
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