SASDZ98

Engineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-07-23T17:37:52.565046+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Engineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))

Concentration— – 5.0 Buffer / pH20 mM HEPES, 150 mM NaCl, pH 7.0 / 7.0
Experimental temperature25.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure0.5 s × 1800

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Fc-L1-CHAP(opt)-LSN(HEK)
View sequence
GPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKASGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGDYAKDIPYANNFNNEAKIYKNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGKLQKFQSLDQNWYGGGERKAEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK
proteinsynthetic construct——–—dimerMolecule count 273.856 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11048[3]0.06180079–2.999013 1/nmHas uncertainty columnMissing 00sasbdb/entries/98/sasdz98/source/SASDZ98.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)40.0—nm
i0Guinier11653.1—arbitrary
i0P(r)12332.32—arbitrary
mwExperimental120.0—kDa
rgGuinier7.30.1nm
rgP(r)8.823—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 150 mM NaCl, pH 7.0Buffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-04-06Storage / measurement temperature25.0 / 25.0
Exposure time0.5Frames1800
Wavelength0.124Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 6M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.062 – 2.999Sample volume / flow rate25.0 / 0.35

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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sascifsasbdb/entries/98/sasdz98/source/SASDZ98.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/98/sasdz98/source/summary.jsondownloaded5348a0e53e306c03160f21d76a30a2fa344f0a60f7a78d3294e5f6355de0cc8ec16eDownloadView source fileSource site
curve:source record
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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:123 field values
Field pathRaw value
codeSASDZ98
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleSEC-SAXS data for Fc-CHAP-LSN
project.publicationnull
project.statusreleased
project.submitted_date2026-05-13
project.released_date2026-07-23
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 6M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFc-L1-CHAP(opt)-LSN(HEK)
experiment.sample.molecule[0].short_nameL1829
experiment.sample.molecule[0].sequenceGPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKAEAAAKEAAAKEAAAKEAAAKALEAEAAAKEAAAKEAAAKEAAAKASGSSTLNYLKSLEGKGWDFDGSFGWQCFDLVNVYWNHLYGHGLKGDYAKDIPYANNFNNEAKIYKNTPTFKAEPGDLVVFSGRYGGGYGHTAIVLNGNYDGKLQKFQSLDQNWYGGGERKAEVAHKVVHNYENDMIFIRPFKKAGGAATHEHSAQWLNNYKKGYGYGPYPLGINGGMHYGVDFFMNIGTPVKAISSGKIVEAGWSNYGGGNQIGLIENDGVHRQWYMHLSKYNVKVGDYVKAGQIIGWSGSTGYSTAPHLHFQRMVNSFSNPTAQDPMPFLKSAGYGKAGGTVTPTPNTGWKTNKYGTLYKSESASFTPNTDIITRTTGPFRSMPQSGVLKAGQTIHYDEVMKQDGHVWVGYTGNSGQRIYLPVRTWQKSTNTLGVLWGTIK
experiment.sample.molecule[0].organismsynthetic construct
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw73.856
experiment.sample.molecule[0].total_mw147.712
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionhuman IgG1 Fc domain connected via a helical linker to a genetic fusion of the CHAP domain of an engineered derivative of S.aureus LytN and an engineered derivative of S.simulans lysostaphin
experiment.sample.buffer.name20 mM HEPES, 150 mM NaCl, pH 7.0
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameEngineered fusion of derivative of S.aureus LytN (CHAP domain) and derivative of S.simulans lysostaphin (Fc-L1-CHAP(opt)-LSN(HEK))
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-04-06
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time0.5
experiment.number_of_frames1800
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume25.0
experiment.flow_rate0.35
experiment.s_min0.062
experiment.s_max2.999
experiment.total_exposure_timenull
experiment.seccolumn49
fits[]
estimated_volume_methodnull
pddf_softwareOther
pddf_software_versionGNOM
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i012332.32
pddf_i0_errornull
guinier_i011653.1
guinier_i0_errornull
pddf_rg8.823
pddf_rg_errornull
guinier_rg7.3
guinier_rg_error0.1
pddf_dmax40.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first12
guinier_point_last32
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZ98_datloglog_img.png
symmetrynull
last_modified2026-07-23T17:37:52.565046+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDZ98
statussuccess
started_at2026-08-11T15:48:49.272770+00:00
finished_at2026-08-11T15:48:56.774887+00:00
source_last_modified2026-07-23T17:37:52.565046+02:00
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View the complete summary.json source
{
  "code": "SASDZ98",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "SEC-SAXS data for Fc-CHAP-LSN",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-05-13",
    "released_date": "2026-07-23"
  },
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}
View the complete manifest.json source
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  "status": "success",
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