Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 1:1 (2nd peak)

{
  "code": "SASDZD8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-05-18",
    "released_date": "2026-07-28"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDZD8.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDZD8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZD8_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZD8_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDZD8.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "SOLEIL",
      "city": "Saint-Aubin",
      "country": "France",
      "beamline_name": "SWING",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Venom Allergen-like protein 11",
          "short_name": "SmVAL11",
          "sequence": "MSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGEVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGKACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETLTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACKEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWADHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSIQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKKAIK",
          "organism": "Schistosoma mansoni",
          "uniprot_code": "Q1WL54",
          "uniprot_range_first": 1,
          "uniprot_range_last": 423,
          "oligomerization": "other",
          "molecular_type": "protein",
          "uniprot_sequence": "MSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGE\nVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGK\nACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETL\nTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACK\nEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWAD\nHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSI\nQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKK\nAIK",
          "mw": 47.0,
          "total_mw": 47.0,
          "number_molecules": 3,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) is a 47 kDa protein composed by two symmetrical domains  separated by a flexible linker. The latter, in our experience, undergoes autocleavage in vitro, with an unknown mechanism."
        },
        {
          "long_name": "Polyclonal antibody anti-SmVAL11",
          "short_name": "pAb anti-SmVAL11",
          "sequence": "A",
          "organism": "Lagomorph",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "",
          "mw": 0.089,
          "total_mw": 140.0,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Polyclonal antibody raised against SmVAL11 in rabbit."
        }
      ],
      "buffer": {
        "name": "25 mM MES, 125 mM NaCl, 1 mM EDTA",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 6.8,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) in complex with pAb anti-SmVAL11, ratio 1:1 (2nd peak)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "University of Lyon",
            "webpage": null
          }
        ],
        "contributor_name": "Adriana Erica",
        "contributor_surname": "Miele",
        "orcid": "http://orcid.org/0000-0002-4637-2606"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2025-01-22",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 2600,
    "wavelength": 0.76,
    "sample_detector_distance": 2.0,
    "concentration_min": null,
    "concentration_max": 2.7,
    "sample_volume": 45.0,
    "flow_rate": 0.3,
    "s_min": 0.068,
    "s_max": 5.529,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "SmVAL11 in complex with its cognate pAb at a 1:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. The present analysis focuses on the second peak.",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 146.8,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 169.2,
  "porod_mw_error": null,
  "pddf_i0": 1.41,
  "pddf_i0_error": null,
  "guinier_i0": 1.40187,
  "guinier_i0_error": null,
  "pddf_rg": 4.99,
  "pddf_rg_error": null,
  "guinier_rg": 4.874,
  "guinier_rg_error": 0.019,
  "pddf_dmax": 16.4,
  "pddf_dmax_error": null,
  "porod_volume": 204.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 10,
  "guinier_point_last": 39,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDZD8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2026-07-28T14:42:19.875272+02:00",
  "bragg_peak": []
}

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  "started_at": "2026-08-11T15:51:21.416331+00:00",
  "finished_at": "2026-08-11T15:51:29.178054+00:00",
  "source_last_modified": "2026-07-28T14:42:19.875272+02:00",
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