SASDZH8

Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) - Full length

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-07-28T14:42:38.667014+02:00

SmVAL11 is a 45 kDa protein containing two SCP/TAPS domains arranged in tandem and connected by a flexible linker. The protein is prone to autocleavage at the linker region, resulting in a heterogeneous sample containing multiple species in solution. To address this, SAXS measurements were coupled to Size Exclusion Chromatography (SEC-SAXS) to separate and individually analyse the different populations. The scattering data presented here were obtained by Evolving Factor Analysis (EFA) deconvolution of the most intense elution peak, as implemented in RAW, and likely correspond to the full-length SmVAL11 protein.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Schistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) - Full length

Concentration— – 11.0 Buffer / pH25 mM MES, 125 mM NaCl, 1 mM EDTA / 6.8
Experimental temperature20.0 Facility / beamlineSOLEIL / SWING
Wavelength0.76 nmExposure1.0 s × 2400

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Venom Allergen-like protein 11
View sequence
MSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGEVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGKACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETLTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACKEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWADHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSIQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKKAIK
proteinSchistosoma mansoniQ1WL541–423otherMolecule count 347.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11218[2,5]0.0–0.552915 1/ANo uncertainty columnMissing 1035sasbdb/entries/h8/sasdzh8/source/SASDZH8.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)18.0—Å
i0Guinier1.52905——
i0P(r)1.553——
mwExperimental40.2—kDa
mwPorod49.2—kDa
porod_volumePorod59.3—ų
rgGuinier3.9080.015Å
rgP(r)4.22—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM MES, 125 mM NaCl, 1 mM EDTABuffer concentration—
pH6.8Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2024-07-11Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames2400
Wavelength0.76Sample-to-detector distance2.0
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.041 – 5.529Sample volume / flow rate45.0 / 0.3

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:133 field values
Field pathRaw value
codeSASDZH8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSchistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)
project.publicationnull
project.statusreleased
project.submitted_date2026-05-18
project.released_date2026-07-28
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pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZH8_pofr_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameVenom Allergen-like protein 11
experiment.sample.molecule[0].short_nameSmVAL11
experiment.sample.molecule[0].sequenceMSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGEVGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGKACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETLTESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACKEQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWADHLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSIQGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKKAIK
experiment.sample.molecule[0].organismSchistosoma mansoni
experiment.sample.molecule[0].uniprot_codeQ1WL54
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last423
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSRLDDFREDCFRAHNEKRLLHGVCALRHSLALDKTAQDWAEALLSEDGIKNSPLSSRGE VGESISVRTSTGTHVDMQGHEVVNTWHSDAENYNYENGKGPAGNFTQLVWSSTREVGFGK ACGPGKCVVVAHYRPPGNVLGRYLENVFRPKKSVKDVKQPIRNTFALNNDTPKTVITETL TESDGKQYSVRREISDLTDDKGKNKKLYLVGQGKKVEYFSCCFNNRTRRCINEVYTDACK EQKKAKSSISPDGHLVDNSKKLQESIHSVVQLHNQYRSQHGSNPLVLDQNLSNMAQQWAD HLLQQSHLSNSGYVYRGMKVGENLGSRWSNGPMELNCKDLIEHWYQESGKYKFNSEPDSI QGIGNFTQIVWTSSERIGVGIAIQSYKSGEDLHKDSKMILVCLYHPPGNVISQFQNNVKK AIK
experiment.sample.molecule[0].mw47.0
experiment.sample.molecule[0].total_mw47.0
experiment.sample.molecule[0].number_molecules3
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionSchistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) is a 47 kDa protein composed by two symmetrical domains separated by a flexible linker. The latter, in our experience, undergoes autocleavage in vitro, with an unknown mechanism.
experiment.sample.buffer.name25 mM MES, 125 mM NaCl, 1 mM EDTA
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSchistosoma mansoni Venom Allergen-like protein isoform 11 (SmVAL11) - Full length
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[]
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2024-07-11
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames2400
experiment.wavelength0.76
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max11.0
experiment.sample_volume45.0
experiment.flow_rate0.3
experiment.s_min0.041
experiment.s_max5.529
experiment.total_exposure_timenull
experiment.seccolumn14
fits[0].models[]
fits[0].fit_unit1/A
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fits[0].softwareDAMMIF
fits[0].chi_square_value2.607
fits[0].p_value0.0
fits[0].fit_residual_plotSASDZH8_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSmVAL11 is a 45 kDa protein containing two SCP/TAPS domains arranged in tandem and connected by a flexible linker. The protein is prone to autocleavage at the linker region, resulting in a heterogeneous sample containing multiple species in solution. To address this, SAXS measurements were coupled to Size Exclusion Chromatography (SEC-SAXS) to separate and individually analyse the different populations. The scattering data presented here were obtained by Evolving Factor Analysis (EFA) deconvolution of the most intense elution peak, as implemented in RAW, and likely correspond to the full-length SmVAL11 protein.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw40.2
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_error0.001
porod_mw49.2
porod_mw_errornull
pddf_i01.553
pddf_i0_errornull
guinier_i01.52905
guinier_i0_errornull
pddf_rg4.22
pddf_rg_errornull
guinier_rg3.908
guinier_rg_error0.015
pddf_dmax18.0
pddf_dmax_errornull
porod_volume59.3
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first11
guinier_point_last57
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZH8_datloglog_img.png
symmetrynull
last_modified2026-07-28T14:42:38.667014+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDZH8
statussuccess
started_at2026-08-11T15:54:09.758589+00:00
finished_at2026-08-11T15:54:17.622435+00:00
source_last_modified2026-07-28T14:42:38.667014+02:00
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View the complete summary.json source
{
  "code": "SASDZH8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Schistosoma mansoni Venom Allergen-like protein 11 (SmVAL11) in complex with cognate polyclonal antibody (pAb anti-SmVAL11)",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-05-18",
    "released_date": "2026-07-28"
  },
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      "country": "France",
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          "long_name": "Venom Allergen-like protein 11",
          "short_name": "SmVAL11",
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}
View the complete manifest.json source
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