SASDZM8

Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (1st peak)

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-07-28T14:41:05.276570+02:00

SmVAL13 in complex with its cognate pAb at a 2:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. The present analysis focuses on the first peak.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (1st peak)

Concentration— – 3.3 Buffer / pH25 mM MES, 125 mM NaCl, 1 mM EDTA / 6.8
Experimental temperature20.0 Facility / beamlineSOLEIL / SWING
Wavelength0.76 nmExposure1.0 s × 2600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Venom allergen-like protein 13
View sequence
MVDEQLNHDALNEHNRLRALHGCPPLKYDRRLAREAQAWAENLARLKIMKHSICDEYGENLASAQSTGKAEMTGARATRNWYDEIHYHNFNKQFQSQSGHFTQLIWKNTSKAGFGIQHSVDGHHVFIVGRYEPPGNVNGQFLENVPPPIHGQSTPKSKVPSYKHNEQNGPRRTYQDELVIVRETDRKDHNGSNHITLIDSSKRSRSDETIPNKNEVRIIRAEKKRQRRCAKRCSIM
proteinSchistosoma mansoniQ1WIQ51–236monomerMolecule count 127.225 kDa
Polyclonal antibody anti-SmVAL13
View sequence
A
proteinLagomorph——–—monomerMolecule count 1140.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11206[3]0.00501735525–0.552925219 1/AHas uncertainty columnMissing 0438sasbdb/entries/m8/sasdzm8/source/SASDZM8.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)36.9—Å
i0Guinier0.481362——
i0P(r)0.5247——
mwExperimental646.8—kDa
mwPorod792.5—kDa
porod_volumePorod955.0—ų
rgGuinier9.0410.311Å
rgP(r)10.51—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM MES, 125 mM NaCl, 1 mM EDTABuffer concentration—
pH6.8Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2025-01-22Storage / measurement temperature4.0 / 20.0
Exposure time1.0Frames2600
Wavelength0.76Sample-to-detector distance2.0
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineSOLEIL / SWING · Saint-Aubin, France
q range0.05 – 5.529Sample volume / flow rate45.0 / 0.3

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:146 field values
Field pathRaw value
codeSASDZM8
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleSchistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)
project.publicationnull
project.statusreleased
project.submitted_date2026-05-18
project.released_date2026-07-28
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameSOLEIL
experiment.instrument.citySaint-Aubin
experiment.instrument.countryFrance
experiment.instrument.beamline_nameSWING
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameVenom allergen-like protein 13
experiment.sample.molecule[0].short_nameSmVAL13
experiment.sample.molecule[0].sequenceMVDEQLNHDA LNEHNRLRAL HGCPPLKYDR RLAREAQAWA ENLARLKIMK HSICDEYGEN LASAQSTGKA EMTGARATRN WYDEIHYHNF NKQFQSQSGH FTQLIWKNTS KAGFGIQHSV DGHHVFIVGR YEPPGNVNGQ FLENVPPPIH GQSTPKSKVP SYKHNEQNGP RRTYQDELVI VRETDRKDHN GSNHITLIDS SKRSRSDETI PNKNEVRIIR AEKKRQRRCA KRCSIM
experiment.sample.molecule[0].organismSchistosoma mansoni
experiment.sample.molecule[0].uniprot_codeQ1WIQ5
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last236
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMVDEQLNHDALNEHNRLRALHGCPPLKYDRRLAREAQAWAENLARLKIMEHSICDEYGEN LASAQSTGKAEMTGARATRNWYDEIHYHNFNKQFQSQSGHFTQLIWKNTSKAGFGIQHSV DGHHVFIVGRYEPPGNVNGQFLENVPPPIHGQSTPKSKVPSYKHNEQNGPRRTYQDELVI VRETDRKDHNGSNHITLIDSSKRSRSDETIPNKNEVRIIRAEKKRQRRCAKRCSIM
experiment.sample.molecule[0].mw27.225
experiment.sample.molecule[0].total_mw27.225
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionSCP/TAPS domain flanked by intrinsically disordered terminal regions
experiment.sample.molecule[1].long_namePolyclonal antibody anti-SmVAL13
experiment.sample.molecule[1].short_namepAb anti-SmVAL13
experiment.sample.molecule[1].sequenceA
experiment.sample.molecule[1].organismLagomorph
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequence
experiment.sample.molecule[1].mw140.0
experiment.sample.molecule[1].total_mw140.0
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionPolyclonal antibody anti-SmVAL13 raised in rabbit
experiment.sample.buffer.name25 mM MES, 125 mM NaCl, 1 mM EDTA
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameSchistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 2:1 (1st peak)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
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experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversity of Lyon
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameAdriana Erica
experiment.contributor[0].contributor_surnameMiele
experiment.contributor[0].orcidhttp://orcid.org/0000-0002-4637-2606
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2025-01-22
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
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experiment.wavelength0.76
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max3.3
experiment.sample_volume45.0
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experiment.s_min0.05
experiment.s_max5.529
experiment.total_exposure_timenull
experiment.seccolumn48
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSmVAL13 in complex with its cognate pAb at a 2:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. The present analysis focuses on the first peak.
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw646.8
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw792.5
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guinier_i00.481362
guinier_i0_errornull
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guinier_rg9.041
guinier_rg_error0.311
pddf_dmax36.9
pddf_dmax_errornull
porod_volume955.0
porod_volume_errornull
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estimated_volume_errornull
guinier_point_first5
guinier_point_last19
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZM8_datloglog_img.png
symmetrynull
last_modified2026-07-28T14:41:05.276570+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDZM8
statussuccess
started_at2026-08-11T15:56:52.858254+00:00
finished_at2026-08-11T15:56:59.866310+00:00
source_last_modified2026-07-28T14:41:05.276570+02:00
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View the complete summary.json source
{
  "code": "SASDZM8",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)",
    "publication": null,
    "status": "released",
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View the complete manifest.json source
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