SASDZT5

Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2026-07-23T20:40:36.056258+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant

浓度— – 2.0 缓冲液 / pH20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol / 7.0
Experimental temperature设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.0127 nm曝光2.0 s × 750

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem
查看序列
GAMDPMSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKERERRKGLKLGMEQDTWVM
proteinHomo sapiensQ70Z351–377monomer分子数 144.366 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
1908[3]0.01338–0.471169 1/A含误差列缺失 099sasbdb/entries/t5/sasdzt5/source/SASDZT5.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.5Å
i0Guinier11.1429arbitrary
i0P(r)11.170.06arbitrary
mwExperimental42.5kDa
mwPorod45.6kDa
porod_volumePorod55.0ų
rgGuinier2.5830.247Å
rgP(r)2.610.018Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol缓冲液浓度
pH7.0添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2026-02-17储存 / 测量温度— / —
曝光时间2.0帧数750
波长0.0127样品-探测器距离2.1
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.134 – 4.712样品体积 / 流速— / 0.65

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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pddfsasbdb/entries/t5/sasdzt5/source/SASDZT5.outdownloaded82350679ff3fb3475ac4f39da8293184db499f7d3986919883cbf6876e67217b3b806Download查看原文件源站
sascifsasbdb/entries/t5/sasdzt5/source/SASDZT5.sascifnot_availableDownload查看原文件源站
summarysasbdb/entries/t5/sasdzt5/source/summary.jsondownloaded81725a982d63d50c919322f46e094685d01d79b13e25c009debaa624f1a8a33e8322Download查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDZT5
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleFreezing the P-Rex2 DH/PH tandem
project.publicationnull
project.statusreleased
project.submitted_date2026-04-03
project.released_date2026-07-23
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDZT5.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZT5_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZT5_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDZT5_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDZT5_guinier_img.png
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePhosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem
experiment.sample.molecule[0].short_nameP-Rex2 DH/PH tandem
experiment.sample.molecule[0].sequenceGAMDPMSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASKVDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYDEYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLILKELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNITDTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRINTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKERERRKGLKLGMEQDTWVM
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ70Z35
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last377
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSEDSRGDSRAESAKDLEKQLRLRVCVLSELQKTERDYVGTLEFLVSAFLHRMNQCAASK VDKNVTEETVKMLFSNIEDILAVHKEFLKVVEECLHPEPNAQQEVGTCFLHFKDKFRIYD EYCSNHEKAQKLLLELNKIRTIRTFLLNCMLLGGRKNTDVPLEGYLVTPIQRICKYPLIL KELLKRTPRKHSDYAAVMEALQAMKAVCSNINEAKRQMEKLEVLEEWQSHIEGWEGSNIT DTCTEMLMCGVLLKISSGNIQERVFFLFDNLLVYCKRKHRRLKNSKASTDGHRYLFRGRI NTEVMEVENVDDGTADFHSSGHIVVNGWKIHNTAKNKWFVCMAKTPEEKHEWFEAILKER ERRKGLKLGMEQDTWVMISEQGEKLYKMMCRQGNLIKDRKRKLTTFPKCFLGSEFVSWLL EIGEIHRPEEGVHLGQALLENGIIHHVTDKHQFKPEQMLYRFRYDDGTFYPRNEMQDVIS KGVRLYCRLHSLFTPVIRDKDYHLRTYKSVVMANKLIDWLIAQGDCRTREEAMIFGVGLC DNGFMHHVLEKSEFKDEPLLFRFFSDEEMEGSNMKHRLMKHDLKVVENVIAKSLLIKSNE GSYGFGLEDKNKVPIIKLVEKGSNAEMAGMEVGKKIFAINGDLVFMRPFNEVDCFLKSCL NSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCII KVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQKSHSKPPGDEA GDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMI EPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRI SSYKKFSRVLKNRAWPTFKQAKSKISPLHSSDFCPTNCHVNVMEVSYPKTSTSLGSAFGV QLDSRKHNSHDKENKSSEQGKLSPMVYIQHTITTMAAPSGLSLGQQDGHGLRYLLKEEDL ETQDIYQKLLGKLQTALKEVEMCVCQIDDLLSSITYSPKLERKTSEGIIPTDSDNEKGER NSKRVCFNVAGDEQEDSGHDTISNRDSYSDCNSNRNSIASFTSICSSQCSSYFHSDEMDS GDELPLSVRISHDKQDKIHSCLEHLFSQVDSITNLLKGQAVVRAFDQTKYLTPGRGLQEF QQEMEPKLSCPKRLRLHIKQDPWNLPSSVRTLAQNIRKFVEEVKCRLLLALLEYSDSETQ LRRDMVFCQTLVATVCAFSEQLMAALNQMFDNSKENEMETWEASRRWLDQIANAGVLFHF QSLLSPNLTDEQAMLEDTLVALFDLEKVSFYFKPSEEEPLVANVPLTYQAEGSRQALKVY FYIDSYHFEQLPQRLKNGGGFKIHPVLFAQALESMEGYYYRDNVSVEEFQAQINAASLEK VKQYNQKLRAFYLDKSNSPPNSTSKAAYVDKLMRPLNALDELYRLVASFIRSKRTAACAN TACSASGVGLLSVSSELCNRLGACHIIMCSSGVHRCTLSVTLEQAIILARSHGLPPRYIM QATDVMRKQGARVQNTAKNLGVRDRTPQSAPRLYKLCEPPPPAGEE
experiment.sample.molecule[0].mw44.366
experiment.sample.molecule[0].total_mw44.366
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionIsolated P-Rex2 DH/PH tandem with residual linker (GAMDP) from N-terminal TEV cleavage
experiment.sample.buffer.name20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFrozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameUniversity of California, Davis
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameLauren
experiment.contributor[0].contributor_surnameAnderson
experiment.contributor[0].orcidhttp://lauanderson@ucdavis.edu
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2026-02-17
experiment.storage_temperaturenull
experiment.cell_temperaturenull
experiment.exposure_time2.0
experiment.number_of_frames750
experiment.wavelength0.0127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max2.0
experiment.sample_volumenull
experiment.flow_rate0.65
experiment.s_min0.134
experiment.s_max4.712
experiment.total_exposure_timenull
experiment.seccolumn17
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDZT5_fit1_model1_img.png
fits[0].models[0].softwareBILBOMD
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
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fits[0].models[0].model_mw43.75
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fits[0].models[0].lognull
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fits[0].models[0].commentnull
fits[0].models[0].user1310
fits[0].fit_unit1/A
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fits[0].softwareMultiFoXS
fits[0].chi_square_value0.733053
fits[0].p_value0.5882
fits[0].fit_residual_plotSASDZT5_fit1_fitfoxsresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDZT5_fit1.dat
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/feedback_TdHLXVy.log
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw42.5
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw45.6
porod_mw_errornull
pddf_i011.17
pddf_i0_error0.06
guinier_i011.1429
guinier_i0_errornull
pddf_rg2.61
pddf_rg_error0.018
guinier_rg2.583
guinier_rg_error0.247
pddf_dmax8.5
pddf_dmax_errornull
porod_volume55.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last75
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDZT5_datloglog_img.png
symmetrynull
last_modified2026-07-23T20:40:36.056258+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDZT5
statussuccess
started_at2026-08-11T14:56:15.872686+00:00
finished_at2026-08-11T14:56:24.174827+00:00
source_last_modified2026-07-23T20:40:36.056258+02:00
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查看完整 summary.json 原文
{
  "code": "SASDZT5",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Freezing the P-Rex2 DH/PH tandem",
    "publication": null,
    "status": "released",
    "submitted_date": "2026-04-03",
    "released_date": "2026-07-23"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDZT5.out",
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    "sample": {
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        {
          "long_name": "Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem",
          "short_name": "P-Rex2 DH/PH tandem",
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          "organism": "Homo sapiens",
          "uniprot_code": "Q70Z35",
          "uniprot_range_first": 1,
          "uniprot_range_last": 377,
          "oligomerization": "monomer",
          "molecular_type": "protein",
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          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Isolated P-Rex2 DH/PH tandem with residual linker (GAMDP) from N-terminal TEV cleavage"
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
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        "deuteration": null
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      "name": "Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
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    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "University of California, Davis",
            "webpage": null
          }
        ],
        "contributor_name": "Lauren",
        "contributor_surname": "Anderson",
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    "concentration_unit": null,
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    "storage_temperature": null,
    "cell_temperature": null,
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    "concentration_min": null,
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    "seccolumn": 17
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  "last_modified": "2026-07-23T20:40:36.056258+02:00",
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}
查看完整 manifest.json 原文
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