SAXSdb — 10ju

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1. Structure Basics

entry_id	10ju
deposition_date	2026-01-22
last_revision	2026-02-04
title	Crystal Structure of serine/threonine-protein kinase (AEK1) T376D, S395D Mutant from Trypanosoma brucei (AMP-PNP)
keywords	SSGCID, STRUCTURAL GENOMICS, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE, AEK1, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.42 Å
rg_electron	26.48 Å
i0	58303100.00
molecular_weight	59716.0 kDa
excluded_volume	74723 Å³
envelope_volume	92553 Å³
shell_volume	29531 Å³
envelope_diameter	96.0 Å
shell_rg	33.27 Å
envelope_rg	26.54 Å
shape_rg	26.49 Å
total_rg	27.17 Å
total_atoms	4190
n_residues	521
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	93.0 Å
rg_real	27.45 Å
rg_real_error	0.70 Å
i0_real	5.8300e+07
i0_real_error	8.6860e+05
rg_reciprocal	27.44 Å
i0_reciprocal	58300000.0000
total_estimate	0.8771
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	28.9 Å
skewness	0.402
kurtosis	-0.242
angular_range	— – 0.2900 Å⁻¹
current_alpha	0.0000
highest_alpha	19010000.0000
n_real_points	59
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.827; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.983; Smooth: 0.936

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Serine/threonine-protein kinase, putative
formula_weight	41175.8 kDa
ec_number	2.7.1.-
mutation	T376D, S395D
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4278

MEKVTKEDFETIDTLGKGSFAYVVLVRRVGTNNLYAMKVVNKQGLLDHNRCRDVFIERNVLSRINHPFLLKLYWTFQSEHKLFFVMEYMAGGDLDKYMNSVPNKQLDLPTSKLYGAEILMAILTLHEQSVIYRDLKPENILLTGDGHCVLADFGLSKDFYDAKMGENASVTDMRANSFVGSPFYVAPDVLKQREYTNAVDFWSFGILLYRMLCGRTPFSGKNMKEVFDNILYSDLRFPSTVSIPSEAKDLISRLLVKDAAHRIKGPEVKAHPFWTGINFDEVMQKKVKPPRWVPAPSVDKLIAERSAAQSGNNSSKNPAQVVNDPAHSSQLNARQQQLFNGFDCTTDNHLGGGSLEHHHHHH

Source

organism	Trypanosoma brucei brucei TREU927
ncbi_taxonomy_id	185431
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Serine/threonine-protein kinase, putative
formula_weight	41095.8 kDa
ec_number	2.7.1.-
mutation	T376D, S395D
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MEKVTKEDFETIDTLGKGSFAYVVLVRRVGTNNLYAMKVVNKQGLLDHNRCRDVFIERNVLSRINHPFLLKLYWTFQSEHKLFFVMEYMAGGDLDKYMNSVPNKQLDLPTSKLYGAEILMAILTLHEQSVIYRDLKPENILLTGDGHCVLADFGLSKDFYDAKMGENASVTDMRANSFVGSPFYVAPDVLKQREYTNAVDFWSFGILLYRMLCGRTPFSGKNMKEVFDNILYSDLRFPSTVSIPSEAKDLISRLLVKDAAHRIKGPEVKAHPFWTGINFDEVMQKKVKPPRWVPAPSVDKLIAERSAAQSGNNSSKNPAQVVNDPAHSSQLNARQQQLFNGFDCTTDNHLGGGSLEHHHHHH

Source

organism	Trypanosoma brucei brucei TREU927
ncbi_taxonomy_id	185431
host_organism	Escherichia coli BL21(DE3)

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	11

Entity 4 non-polymer

description	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
formula_weight	506.2 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	88

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6. Citations (1)

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length_a	86.538 Å
length_b	88.475 Å
length_c	201.132 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	I 2 2 2
int_tables_number	23
crystal_system	—

resolution	2.150 Å
resolution_low	44.240 Å
r_factor	0.2202
r_work	0.2186
r_free	0.2504

fit	/app/data/saxs_computation/0j/10ju/10ju.fit
pddf	/app/data/pr_computation/0j/10ju/10ju.pr.out
profile	/app/data/saxs_computation/0j/10ju/10ju.dat

Crystal Structure of serine/threonine-protein kinase (AEK1) T376D, S395D Mutant from Trypanosoma brucei (AMP-PNP)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)