10or

Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 316L, 523S and 294S

Method: ELECTRON MICROSCOPY D_max: 164.7 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Envelope glycoprotein 1

Sudan ebolavirus

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Other combination Heteromer Protein × 24 其他Polymer 3 PDB declaration: 24-meric（24） Consistent with protein copy count	Chain A； UniProt 32–235 Chain B； UniProt 510–631 Chain C； UniProt 32–235 Chain D； UniProt 510–631 Chain E； UniProt 32–235 Chain F； UniProt 510–631	Not recorded	316L Heavy Chain × 3 316L Light Chain × 3 523S Heavy Chain × 3 523S Light Chain × 3 294S Heavy Chain × 3 294S Light Chain × 3 ;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 3	ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE	Resolution 2.46 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 10OR	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
10OP Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 545S, 523S and 294S Deposited 2026-01-29	Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 24 PDB declaration: 24-meric	Chain A 32–235（204 aa） Chain B 510–631（122 aa） Chain C 32–235（204 aa） Chain D 510–631（122 aa） Chain E 32–235（204 aa） Chain F 510–631（122 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.63 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	R4QJ45_9MONO
Isoform	—
PDB entities	1, 2
Chains and sequence ranges	Author chain A； PDBConstruct 1–204； UniProt 32–235 Author chain C； PDBConstruct 1–204； UniProt 32–235 Author chain E； PDBConstruct 1–204； UniProt 32–235 Author chain B； PDBConstruct 1–122； UniProt 510–631 Author chain D； PDBConstruct 1–122； UniProt 510–631 Author chain F； PDBConstruct 1–122； UniProt 510–631

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	10or
Deposition date deposition_date	2026-01-29
Structure title title	Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 316L, 523S and 294S
Keywords keywords	SUDV, neutralizing mAbs, VIRAL PROTEIN-IMMUNE SYSTEM complex; VIRAL PROTEIN/IMMUNE SYSTEM
Experimental Method method	ELECTRON MICROSCOPY

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	51.05 Å
Radius of gyration Rg (electron density) rg_electron	50.95 Å
Forward intensity I(0) i0	1632320000.00
Molecular weight molecular_weight	330000.0 kDa
Excluded volume excluded_volume	409840 Å³
Envelope volume envelope_volume	586320 Å³
Hydration-shell volume shell_volume	95755 Å³
Envelope diameter envelope_diameter	166.6 Å
Shell Rg shell_rg	54.44 Å
Envelope Rg envelope_rg	49.87 Å
Shape Rg shape_rg	50.90 Å
Total Rg total_rg	51.20 Å
Total atoms total_atoms	23250
Residues n_residues	2883
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	164.7 Å
Rg (real space) rg_real	50.99 Å
Rg uncertainty (real space) rg_real_error	1.46 Å
I(0) (real space) i0_real	1.6320e+09
I(0) uncertainty (real space) i0_real_error	3.0710e+07
Rg (reciprocal space) rg_reciprocal	51.10 Å
I(0) (reciprocal space) i0_reciprocal	1633000000.0000
Solution quality estimate total_estimate	0.8601
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	63.8 Å
Skewness Skewness skewness	0.298
Kurtosis Kurtosis kurtosis	-0.365
Angular range angular_range	— – 0.1550 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	87320000.0000
Real-space data points n_real_points	32
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.853; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.625

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Description description	Envelope glycoprotein 1
Molecular weight formula_weight	22494.2 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,C,E
Atom count atom_count	23250

SMPLGVVTNSTLEVTEIDQLVCKDHLASTDQLKSVGLNLEGSGVSTDIPSATKRWGFRSGVPPKVVSYEAGEWAENCYNLEIKKPDGSECLPPPPDGVRGFPRCRYVHKAQGTGPCPGDYAFHKDGAFFLYDRLASTVIYRGVNFAEGVIAFLILAKPKETFLQSPPIREAVNYTENTSSYYATSYLEYEIENFGAQHSTTLFK

来源 Source

来源生物 organism	Sudan ebolavirus
NCBI Class编号 ncbi_taxonomy_id	186540
宿主生物 host_organism	Homo sapiens

Entity 2 Entity 2 polymer

Description description	Envelope glycoprotein 2
Molecular weight formula_weight	13982.9 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,D,F

KCNPNLHYWTAQEQHNAAGIAWIPYFGPGAEGIYTEGLMHNQNALVCGLRQLANETTQALQLFLRATTELRTYTILNRKAIDFLLRRWGGTCRILGPDCCIEPHDWTKNITDKINQIIHDFI

来源 Source

来源生物 organism	Sudan ebolavirus
NCBI Class编号 ncbi_taxonomy_id	186540
宿主生物 host_organism	Homo sapiens

Entity 3 Entity 3 polymer

Description description	316L Heavy Chain
Molecular weight formula_weight	13300.0 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,I,K

EERLVESGGGLVQPGGSLRLSCAASGFTFGDYAMHWVRQVPGKSLEWLSGISWSGYTTYSADSVKGRFTISRDNAKNSLYLQMNRLRPEDTGLYYCVTLIGAIGIKDSLDVWGRGVLVTVSS

来源 Source

来源生物 organism	Macaca mulatta
通用名 common_name	Rhesus monkey
NCBI Class编号 ncbi_taxonomy_id	9544
宿主生物 host_organism	Homo sapiens

Entity 4 Entity 4 polymer

Description description	316L Light Chain
Molecular weight formula_weight	11779.8 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,J,L

QSVLTQPPSVSGAPGQRVTVSCSGSSSNIGAGNYVQWYQQLPGTAPKVLIYQTEKRPSGTSDRFSGSKSDTSASLTINGLQSEDEADYYCQVYDSNLNGWVFGGGTRLTVL

来源 Source

来源生物 organism	Macaca mulatta
通用名 common_name	Rhesus monkey
NCBI Class编号 ncbi_taxonomy_id	9544
宿主生物 host_organism	Homo sapiens

Entity 5 Entity 5 polymer

Description description	523S Heavy Chain
Molecular weight formula_weight	13711.2 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,O,Q

QVQLVQSGAELKKPGASVKVSCKASGYTFSDHYVHWVRQAPGQGLEWMGRINPNSGGTNYAQRFLGRVTMTRDASISTAYLDLSSLRSDDTAVYYCARSSSSWSGHYYYYMDVWGKGTTVTVSS

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 6 Entity 6 polymer

Description description	523S Light Chain
Molecular weight formula_weight	11366.5 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N,P,R

QSVLTQPPSVSAAPGQRVTISCSGSTSNIASNFVSWYQQLPGTAPKLLIYDNHKRPSGIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSAGVFGGGTKVTVL

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 7 Entity 7 polymer

Description description	294S Heavy Chain
Molecular weight formula_weight	13835.4 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S,U,W

QVQLQESGPGLVKPSQTLSLTCTVSGGSISSGGYHWSWIRQHPGKGLEWIGNIYYNGRTYYNPSLKSRVTISVDTSENEFSLKLSSVTAADTAVYYCARDRRRPRIGHDYGMDVWGQGTKVIVSS

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 8 Entity 8 polymer

Description description	294S Light Chain
Molecular weight formula_weight	11787.0 kDa
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T,V,X

DIQMTQSPSSLSASVGDRVTISCRASQSISNFLNWYQQKPGQAPKLLIYTASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQIYNTGMYSFGQGTKLEIK

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	human
NCBI Class编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Homo sapiens

Entity 9 Entity 9 branched

Description description	;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
Molecular weight formula_weight	910.8 kDa
Source method src_method	man
Copy count entity_count	3

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	2.46 Å
Resolution测定方法 resolution_method	—
颗粒数 num_particles	467000

fit	/app/data/saxs_computation/0o/10or/10or.fit
pddf	/app/data/pr_computation/0o/10or/10or.pr.out
profile	/app/data/saxs_computation/0o/10or/10or.dat

Cryo-EM structure of Sudan Ebolavirus GP bound by three neutralizing antibodies 316L, 523S and 294S

1. Protein Identity and Related Structures Protein Identity & Related Structures

Envelope glycoprotein 1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

冷冻电镜 EM 3D

6. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 polymer

Polymer Polymer

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Entity 3 Entity 3 polymer

Polymer Polymer

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Entity 4 Entity 4 polymer

Polymer Polymer

来源 Source

Entity 5 Entity 5 polymer

Polymer Polymer

来源 Source

Entity 6 Entity 6 polymer

Polymer Polymer

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Entity 7 Entity 7 polymer

Polymer Polymer

来源 Source

Entity 8 Entity 8 polymer

Polymer Polymer

来源 Source

Entity 9 Entity 9 branched

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)