← Back to search

10rx

SthK closed state at low temperature, cAMP-bound in DOPC nanodiscs

Method: ELECTRON MICROSCOPY D_max: 120.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	10rx
deposition_date	2026-02-04
title	SthK closed state at low temperature, cAMP-bound in DOPC nanodiscs
keywords	cyclic nucleotide-gated channel, lipid modulation, pacemaker channel, TRANSPORT PROTEIN, potassium channel, thermoreceptor; TRANSPORT PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	39.54 Å
rg_electron	38.91 Å
i0	413990000.00
molecular_weight	188980.0 kDa
excluded_volume	246380 Å³
envelope_volume	317210 Å³
shell_volume	66272 Å³
envelope_diameter	118.1 Å
shell_rg	46.41 Å
envelope_rg	37.94 Å
shape_rg	38.91 Å
total_rg	39.40 Å
total_atoms	27540
n_residues	1580
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	120.7 Å
rg_real	39.30 Å
rg_real_error	1.04 Å
i0_real	4.1400e+08
i0_real_error	7.6930e+06
rg_reciprocal	39.45 Å
i0_reciprocal	414100000.0000
total_estimate	0.8977
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	48.3 Å
skewness	0.139
kurtosis	-0.554
angular_range	— – 0.2000 Å⁻¹
current_alpha	0.0000
highest_alpha	28500000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.931; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.981; Smooth: 0.894

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	Transcriptional regulator, Crp/Fnr family
formula_weight	51118.6 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	27540

MAKDIGINSDPNSSSVDKLMKSSGVSNPTYTLVWKVWILAVTLYYAIRIPLTLVFPSLFSPLLPLDILASLALIADIPLDLAFESRRTSGRKPTLLAPSRLPDLLAALPLDLLVFALHLPSPLSLLSLVRLLKLISVQRSATRILSYRINPALLRLLSLVGFILLAAHGIACGWMSLQPPSENPAGTRYLSAFYWTITTLTTIGYGDITPSTPTQTVYTIVIELLGAAMYGLVIGNIASLVSKLDAAKLLHRERVERVTAFLSYKRISPELQRRIIEYFDYLWETRRGYEEREVLKELPHPLRLAVAMEIHGDVIEKVPLFKGAGEEFIRDIILHLEPVIYGPGEYIIRAGEMGSDVYFINRGSVEVLSADEKTRYAILSEGQFFGEMALILRAPRTATVRARAFCDLYRLDKETFDRILSRYPEIAAQIQELAVRRKELESSGLVPRGSVKHHHH

Source

organism	Winmispira thermophila
ncbi_taxonomy_id	154
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
formula_weight	329.2 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE
formula_weight	787.1 kDa
src_method	syn
entity_count	32

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.510 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	2.51 Å
resolution_method	—
num_particles	377840

fit	/app/data/saxs_computation/0r/10rx/10rx.fit
pddf	/app/data/pr_computation/0r/10rx/10rx.pr.out
profile	/app/data/saxs_computation/0r/10rx/10rx.dat

SthK closed state at low temperature, cAMP-bound in DOPC nanodiscs

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D