SAXSdb — 10uh

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Formyl-coenzyme a transferase

Brucella melitensis 16M1W

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 50–450 Chain B； UniProt 50–450	Not recorded	PGE TRIETHYLENE GLYCOL × 3 GOL GLYCEROL × 2 ACT ACETATE ION × 2 COA COENZYME A × 2 PEG DI(HYDROXYETHYL)ETHER × 1	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；Index G7: 0.20M Ammonium Acetate, 0.1M Bis-Tris pH 6.5, 20% PEG 3350. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. Cocrystallization with 2mM CoA. plate 19820 G7 drop 2, Puck: PSL-1916, Cryo: 20% PEG 200 + 80% crystallant	Resolution 1.85 Å R-free 0.191
2	Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain C； UniProt 50–450	Not recorded	PGE TRIETHYLENE GLYCOL × 4 GOL GLYCEROL × 2 ACT ACETATE ION × 2 COA COENZYME A × 2	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；Index G7: 0.20M Ammonium Acetate, 0.1M Bis-Tris pH 6.5, 20% PEG 3350. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. Cocrystallization with 2mM CoA. plate 19820 G7 drop 2, Puck: PSL-1916, Cryo: 20% PEG 200 + 80% crystallant	Resolution 1.85 Å R-free 0.191

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 10UH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
10UI Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with Zinc Deposited 2026-02-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 50–450（401 aa） Chain B 50–450（401 aa）	Not recorded	PEG DI(HYDROXYETHYL)ETHER × 2 ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；PPX D8: 0.2M Magnesium Acetate, 0.1M MES pH 6.5, 15% PEG 6000. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. plate 19824 D8 drop 1, Puck: PSL-1911, Cryo: 20% PEG 200 + 80% crystallant	Resolution 2.34 Å R-free 0.263
10UI Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with Zinc Deposited 2026-02-09	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 50–450（401 aa） Chain D 50–450（401 aa）	Not recorded	ZN ZINC ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；PPX D8: 0.2M Magnesium Acetate, 0.1M MES pH 6.5, 15% PEG 6000. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. plate 19824 D8 drop 1, Puck: PSL-1911, Cryo: 20% PEG 200 + 80% crystallant	Resolution 2.34 Å R-free 0.263
9ZAV Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with succinate Deposited 2025-11-19	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 50–450（401 aa）	Not recorded	SIN SUCCINIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；Index C10: 1.0M Succinic acid pH 7.0, 0.1M HEPES pH 7.0, 1% PEG 200mme. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. Succinate in 3 subunits was acquired from the crystallant. plate 19820 C10 drop 1, Puck: PSL-2010, Cryo: 20% PEG 200 + 80% crystallant	Resolution 2.88 Å R-free 0.225
9ZAV Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with succinate Deposited 2025-11-19	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain B 50–450（401 aa） Chain C 50–450（401 aa）	Not recorded	SIN SUCCINIC ACID × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；Index C10: 1.0M Succinic acid pH 7.0, 0.1M HEPES pH 7.0, 1% PEG 200mme. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. Succinate in 3 subunits was acquired from the crystallant. plate 19820 C10 drop 1, Puck: PSL-2010, Cryo: 20% PEG 200 + 80% crystallant	Resolution 2.88 Å R-free 0.225
9ZAV Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with succinate Deposited 2025-11-19	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain D 50–450（401 aa） Chain E 50–450（401 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；Index C10: 1.0M Succinic acid pH 7.0, 0.1M HEPES pH 7.0, 1% PEG 200mme. BrmeA.18114.b.B2.PW39356 at 20.7 mg/mL. Succinate in 3 subunits was acquired from the crystallant. plate 19820 C10 drop 1, Puck: PSL-2010, Cryo: 20% PEG 200 + 80% crystallant	Resolution 2.88 Å R-free 0.225

2 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	Q8YDF2_BRUME
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 9–409； UniProt 50–450 Author chain B； PDBConstruct 9–409； UniProt 50–450 Author chain C； PDBConstruct 9–409； UniProt 50–450

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	10uh
Deposition date deposition_date	2026-02-09
最后修订 last_revision	2026-02-18
Structure title title	Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA
Keywords keywords	SSGCID, STRUCTURAL GENOMICS, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE, TRANSFERASE, Formyl-coenzyme A transferase; TRANSFERASE
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	37.84 Å
Radius of gyration Rg (electron density) rg_electron	37.73 Å
Forward intensity I(0) i0	277106000.00
Molecular weight molecular_weight	133300.0 kDa
Excluded volume excluded_volume	166420 Å³
Envelope volume envelope_volume	215040 Å³
Hydration-shell volume shell_volume	48974 Å³
Envelope diameter envelope_diameter	136.1 Å
Shell Rg shell_rg	41.93 Å
Envelope Rg envelope_rg	38.21 Å
Shape Rg shape_rg	37.74 Å
Total Rg total_rg	37.94 Å
Total atoms total_atoms	9348
Residues n_residues	1209
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	128.6 Å
Rg (real space) rg_real	38.14 Å
Rg uncertainty (real space) rg_real_error	1.13 Å
I(0) (real space) i0_real	2.7710e+08
I(0) uncertainty (real space) i0_real_error	4.8080e+06
Rg (reciprocal space) rg_reciprocal	37.96 Å
I(0) (reciprocal space) i0_reciprocal	277100000.0000
Solution quality estimate total_estimate	0.8446
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	36.8 Å
Skewness Skewness skewness	0.542
Kurtosis Kurtosis kurtosis	-0.183
Angular range angular_range	— – 0.2100 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	52470000.0000
Real-space data points n_real_points	43
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.806; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.950; Smooth: 0.608

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Formyl-coenzyme a transferase
Molecular weight formula_weight	44101.3 kDa
EC 编号 ec_number	2.8.3.-
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C
Atom count atom_count	10148

MAHHHHHHALPLAGIKVLDLSRVLAGPWATMSLADMGAEVWKIENIQGGDDTRAWSVPNYKGASTYFLCANRGKKSLALDLKSREGLEIIHELAKQADVVVENFRSGTVERLKIDYESLKALNPGIVYCSISGYGQTGPEAQRPGYDFVVQAESGLMSITGQIDGEPTRIGVAMTDIVAGMVATQSVLAALYQRKTTGLGQYIDVSLYECALNTLINVGSAHLNGGHVPARFGNAHPTVVPYQIFECSDGAFALAVGNDRQFAILCERIIDLPELAADERFKTASGRALNRAALIPPMAERFRTNTRQHWMSACLKMGVPAGQVKTVPEAFESPNVKARQVVQKLESAHLGPISLVRPAQGLKAQENAAYKAPPMLGEDSASVLGDVLGLDGNKLADLIAAGVIYQYQP

来源 Source

来源生物 organism	Brucella melitensis 16M1W
NCBI Class编号 ncbi_taxonomy_id	1143190
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 non-polymer

Description description	TRIETHYLENE GLYCOL
Molecular weight formula_weight	150.2 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 3 Entity 3 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 4 Entity 4 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 5 Entity 5 non-polymer

Description description	COENZYME A
Molecular weight formula_weight	767.5 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 6 Entity 6 non-polymer

Description description	DI(HYDROXYETHYL)ETHER
Molecular weight formula_weight	106.1 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	701

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8. Citations (1)

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Unit-cell length a length_a	95.054 Å
Unit-cell length b length_b	176.355 Å
Unit-cell length c length_c	213.183 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	24

Space group space_group	C 2 2 21
Space-group number int_tables_number	20
Crystal system crystal_system	—

Resolution resolution	1.850 Å
Low-resolution limit resolution_low	48.680 Å
R factor r_factor	0.1754
R_work r_work	0.1746
R_free r_free	0.1912

fit	/app/data/saxs_computation/0u/10uh/10uh.fit
pddf	/app/data/pr_computation/0u/10uh/10uh.pr.out
profile	/app/data/saxs_computation/0u/10uh/10uh.dat

Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Formyl-coenzyme a transferase

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction