10uh

Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Formyl-coenzyme a transferase

Brucella melitensis 16M1W

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	TRIETHYLENE GLYCOL × 3 GLYCEROL × 2 ACETATE ION × 2 COENZYME A × 2 DI(HYDROXYETHYL)ETHER × 1 water × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	TRIETHYLENE GLYCOL × 4 GLYCEROL × 2 ACETATE ION × 2 COENZYME A × 2 water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q8YDF2_BRUME
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 9–409； UniProt 50–450 Author chain B； PDBConstruct 9–409； UniProt 50–450 Author chain C； PDBConstruct 9–409； UniProt 50–450

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	10uh
Deposition date deposition_date	2026-02-09
Last revision last_revision	2026-02-18
Structure title title	Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA
Keywords keywords	SSGCID, STRUCTURAL GENOMICS, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE, TRANSFERASE, Formyl-coenzyme A transferase; TRANSFERASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

10uh__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

10uh__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

10uh__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	27.22 Å
Rg (electron density)	26.25 Å
Total Rg	27.01 Å
Atom count	6231
Residues	806
Excluded volume	110920 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`10uh__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`10uh__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Description description	Formyl-coenzyme a transferase
Molecular weight formula_weight	44101.3 kDa
EC 编号 ec_number	2.8.3.-
Source method src_method	man
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C
Atom count atom_count	10148

MAHHHHHHALPLAGIKVLDLSRVLAGPWATMSLADMGAEVWKIENIQGGDDTRAWSVPNYKGASTYFLCANRGKKSLALDLKSREGLEIIHELAKQADVVVENFRSGTVERLKIDYESLKALNPGIVYCSISGYGQTGPEAQRPGYDFVVQAESGLMSITGQIDGEPTRIGVAMTDIVAGMVATQSVLAALYQRKTTGLGQYIDVSLYECALNTLINVGSAHLNGGHVPARFGNAHPTVVPYQIFECSDGAFALAVGNDRQFAILCERIIDLPELAADERFKTASGRALNRAALIPPMAERFRTNTRQHWMSACLKMGVPAGQVKTVPEAFESPNVKARQVVQKLESAHLGPISLVRPAQGLKAQENAAYKAPPMLGEDSASVLGDVLGLDGNKLADLIAAGVIYQYQP

Source Source

Source organism organism	Brucella melitensis 16M1W
NCBI taxonomy ID ncbi_taxonomy_id	1143190
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 non-polymer

Description description	TRIETHYLENE GLYCOL
Molecular weight formula_weight	150.2 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 3 Entity 3 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 4 Entity 4 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 5 Entity 5 non-polymer

Description description	COENZYME A
Molecular weight formula_weight	767.5 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 6 Entity 6 non-polymer

Description description	DI(HYDROXYETHYL)ETHER
Molecular weight formula_weight	106.1 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 7 Entity 7 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	701

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Unit-cell length a length_a	95.054 Å
Unit-cell length b length_b	176.355 Å
Unit-cell length c length_c	213.183 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	24

Space group space_group	C 2 2 21
Space-group number int_tables_number	20
Crystal system crystal_system	—

Resolution resolution	1.850 Å
Low-resolution limit resolution_low	48.680 Å
R factor r_factor	0.1754
R_work r_work	0.1746
R_free r_free	0.1912

Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Formyl-coenzyme a transferase

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

10uh__assembly_1__model_1

10uh__assembly_1__model_1 | I(q)

10uh__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 water

7. Citations (1)

Crystal structure of Formyl-coenzyme A transferase from Brucella melitensis in complex with CoA

1. Protein Identity and Related Structures Protein Identity & Related Structures

Formyl-coenzyme a transferase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

10uh__assembly_1__model_1

10uh__assembly_1__model_1 | I(q)

10uh__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (7)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 non-polymer

Entity 7 Entity 7 water

7. Citations (1)