Cryo EM structure of glutamine synthetase from Brucella melitensis

Method: ELECTRON MICROSCOPY D_max: 165.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	11zs
deposition_date	2026-03-20
title	Cryo EM structure of glutamine synthetase from Brucella melitensis
keywords	;SSGCID, STRUCTURAL GENOMICS, SEATTLE STRUCTURAL GENOMICS CENTER FOR INFECTIOUS DISEASE, glutamine synthetase, Brucella melitensis, LIGASE ;; LIGASE
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	55.44 Å
rg_electron	55.01 Å
i0	4907760000.00
molecular_weight	594570.0 kDa
excluded_volume	743940 Å³
envelope_volume	1038000 Å³
shell_volume	145440 Å³
envelope_diameter	168.5 Å
shell_rg	67.78 Å
envelope_rg	53.35 Å
shape_rg	55.02 Å
total_rg	55.26 Å
total_atoms	41952
n_residues	5196
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	165.0 Å
rg_real	55.02 Å
rg_real_error	1.34 Å
i0_real	4.9080e+09
i0_real_error	9.2380e+07
rg_reciprocal	55.79 Å
i0_reciprocal	4913000000.0000
total_estimate	0.8714
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	76.6 Å
skewness	-0.056
kurtosis	-0.559
angular_range	— – 0.1400 Å⁻¹
current_alpha	0.0000
highest_alpha	458100000.0000
n_real_points	29
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.911; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.937; Smooth: 0.655

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4. Crystallography & Experiment

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5. Entities & Polymer Info (1)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.280 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.28 Å
resolution_method	—
num_particles	71284

description	Glutamine synthetase, catalytic domain
formula_weight	52725.6 kDa
ec_number	6.3.1.2
src_method	man
entity_count	12

Cryo EM structure of glutamine synthetase from Brucella melitensis

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (1)

Entity 1 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

type	polypeptide(L)
strand_id	A,B,C,D,E,F,G,H,I,J,K,L
atom_count	41952

organism	Brucella abortus 2308
ncbi_taxonomy_id	359391
host_organism	Escherichia coli BL21(DE3)

fit	/app/data/saxs_computation/1z/11zs/11zs.fit
pddf	/app/data/pr_computation/1z/11zs/11zs.pr.out
profile	/app/data/saxs_computation/1z/11zs/11zs.dat