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1a25

C2 DOMAIN FROM PROTEIN KINASE C (BETA)

Method: X-RAY DIFFRACTION D_max: 77.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a25
deposition_date	1998-01-16
title	C2 DOMAIN FROM PROTEIN KINASE C (BETA)
keywords	CALCIUM++/PHOSPHOLIPID BINDING PROTEIN, CALCIUM-BINDING PROTEIN; CALCIUM-BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.69 Å
rg_electron	21.08 Å
i0	16833100.00
molecular_weight	31215.0 kDa
excluded_volume	39164 Å³
envelope_volume	45726 Å³
shell_volume	19059 Å³
envelope_diameter	77.9 Å
shell_rg	26.93 Å
envelope_rg	21.52 Å
shape_rg	21.10 Å
total_rg	21.84 Å
total_atoms	2190
n_residues	264
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	77.5 Å
rg_real	21.87 Å
rg_real_error	0.68 Å
i0_real	1.6830e+07
i0_real_error	2.4460e+05
rg_reciprocal	21.83 Å
i0_reciprocal	16830000.0000
total_estimate	0.8207
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	22.0 Å
skewness	0.609
kurtosis	0.079
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	3932000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.636; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.771; Smooth: 0.985

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN KINASE C (BETA)
formula_weight	16882.1 kDa
ec_number	2.7.1.-
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	2239

GSPGISGGGGGILDSMERRGRIYIQAHIDREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVP

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	6

Entity 3 non-polymer

description	O-PHOSPHOETHANOLAMINE
formula_weight	171.1 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	49

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6. Citations (1)

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length_a	77.798 Å
length_b	77.798 Å
length_c	140.826 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 43 21 2
int_tables_number	96
crystal_system	—

resolution	2.700 Å
resolution_low	20.000 Å
r_factor	0.2220
r_work	0.2220
r_free	0.2540

fit	/app/data/saxs_computation/a2/1a25/1a25.fit
pddf	/app/data/pr_computation/a2/1a25/1a25.pr.out
profile	/app/data/saxs_computation/a2/1a25/1a25.dat

C2 DOMAIN FROM PROTEIN KINASE C (BETA)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction