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1a5z

LACTATE DEHYDROGENASE FROM THERMOTOGA MARITIMA (TMLDH)

Method: X-RAY DIFFRACTION D_max: 65.4 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a5z
deposition_date	1998-02-18
title	LACTATE DEHYDROGENASE FROM THERMOTOGA MARITIMA (TMLDH)
keywords	OXIDOREDUCTASE, GLYCOLYSIS, HYPERTHERMOPHILES, THERMOTOGA MARITIMA, PROTEIN STABILITY; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.09 Å
rg_electron	19.88 Å
i0	23477100.00
molecular_weight	35979.0 kDa
excluded_volume	44624 Å³
envelope_volume	52226 Å³
shell_volume	21856 Å³
envelope_diameter	66.4 Å
shell_rg	26.47 Å
envelope_rg	20.10 Å
shape_rg	19.87 Å
total_rg	20.78 Å
total_atoms	2508
n_residues	312
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	65.4 Å
rg_real	20.98 Å
rg_real_error	0.33 Å
i0_real	2.3480e+07
i0_real_error	2.7230e+05
rg_reciprocal	21.00 Å
i0_reciprocal	23480000.0000
total_estimate	0.9033
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	26.0 Å
skewness	0.200
kurtosis	-0.410
angular_range	— – 0.3750 Å⁻¹
current_alpha	0.0000
highest_alpha	3560000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.921; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.981

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	L-LACTATE DEHYDROGENASE
formula_weight	35026.4 kDa
ec_number	1.1.1.27
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2852

MKIGIVGLGRVGSSTAFALLMKGFAREMVLIDVDKKRAEGDALDLIHGTPFTRRANIYAGDYADLKGSDVVIVAAGVPQKPGETRLQLLGRNARVMKEIARNVSKYAPDSIVIVVTNPVDVLTYFFLKESGMDPRKVFGSGTVLDTARLRTLIAQHCGFSPRSVHVYVIGEHGDSEVPVWSGAMIGGIPLQNMCQVCQKCDSKILENFAEKTKRAAYEIIERKGATHYAIALAVADIVESIFFDEKRVLTLSVYLEDYLGVKDLCISVPVTLGKHGVERILELNLNEEELEAFRKSASILKNAINEITAEENKHQNTSG

Source

organism	Thermotoga maritima
ncbi_taxonomy_id	2336
host_organism	Escherichia coli

Entity 2 non-polymer

description	1,6-di-O-phosphono-beta-D-fructofuranose
formula_weight	340.1 kDa
src_method	man
entity_count	2

Entity 3 non-polymer

description	CADMIUM ION
formula_weight	112.4 kDa
src_method	syn
entity_count	3

Entity 4 non-polymer

description	OXAMIC ACID
formula_weight	89.0 kDa
src_method	syn
entity_count	1

Entity 5 non-polymer

description	NICOTINAMIDE-ADENINE-DINUCLEOTIDE
formula_weight	663.4 kDa
src_method	syn
entity_count	1

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	344

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6. Citations (2)

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length_a	105.480 Å
length_b	105.480 Å
length_c	187.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 41 2 2
int_tables_number	98
crystal_system	—

resolution	2.100 Å
resolution_low	8.000 Å
r_factor	0.2000
r_work	0.2000
r_free	0.2860

fit	/app/data/saxs_computation/a5/1a5z/1a5z.fit
pddf	/app/data/pr_computation/a5/1a5z/1a5z.pr.out
profile	/app/data/saxs_computation/a5/1a5z/1a5z.dat