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1apx

CRYSTAL STRUCTURE OF RECOMBINANT ASCORBATE PEROXIDASE

Method: X-RAY DIFFRACTION D_max: 112.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1apx
deposition_date	1995-02-01
title	CRYSTAL STRUCTURE OF RECOMBINANT ASCORBATE PEROXIDASE
keywords	PEROXIDASE; PEROXIDASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	34.92 Å
rg_electron	34.12 Å
i0	177945000.00
molecular_weight	110770.0 kDa
excluded_volume	139760 Å³
envelope_volume	171460 Å³
shell_volume	41606 Å³
envelope_diameter	115.5 Å
shell_rg	41.03 Å
envelope_rg	33.75 Å
shape_rg	34.11 Å
total_rg	34.65 Å
total_atoms	7836
n_residues	996
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	112.7 Å
rg_real	34.87 Å
rg_real_error	0.81 Å
i0_real	1.7790e+08
i0_real_error	2.9380e+06
rg_reciprocal	34.90 Å
i0_reciprocal	178000000.0000
total_estimate	0.8992
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	47.6 Å
skewness	0.195
kurtosis	-0.632
angular_range	— – 0.2250 Å⁻¹
current_alpha	0.0000
highest_alpha	133700000.0000
n_real_points	46
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.925; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.922

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	CYTOSOLIC ASCORBATE PEROXIDASE
formula_weight	27097.6 kDa
ec_number	1.11.1.11
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D
atom_count	8481

GKSYPTVSPDYQKAIEKAKRKLRGFIAEKKCAPLILRLAWHSAGTFDSKTKTGGPFGTIKHQAELAHGANNGLDIAVRLLEPIKEQFPIVSYADFYQLAGVVAVEITGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLTGEKDGLLQLPSDKALLTDSVFRPLVEKYAADEDVFFADYAEAHLKLSELGFAEA

Source

organism	Pisum sativum
common_name	pea
ncbi_taxonomy_id	3888
host_organism	Escherichia coli

Entity 2 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	PROTOPORPHYRIN IX CONTAINING FE
formula_weight	616.5 kDa
src_method	syn
entity_count	4

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	645

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6. Citations (2)

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length_a	132.400 Å
length_b	53.000 Å
length_c	170.600 Å
angle_alpha	90.00 °
angle_beta	107.10 °
angle_gamma	90.00 °
z_pdb	16

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.200 Å
resolution_low	8.000 Å
r_factor	0.1930
r_work	0.1930
r_free	—

fit	/app/data/saxs_computation/ap/1apx/1apx.fit
pddf	/app/data/pr_computation/ap/1apx/1apx.pr.out
profile	/app/data/saxs_computation/ap/1apx/1apx.dat