1auu

SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES

Method: SOLUTION NMR D_max: 43.5 Å Quality: GOOD

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1. Protein Identity and Related Structures Protein Identity & Related Structures

SACY

Bacillus subtilis

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 1–55 Chain B； UniProt 1–55	Fragment：RNA BINDING DOMAIN, RESIDUES 1 - 55	No other associated polymer	SOLUTION NMR NMR measurement conditions：pH 6；297 K	Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	SACY_BACSU
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–55； UniProt 1–55 Author chain B； PDBConstruct 1–55； UniProt 1–55

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1auu
Deposition date deposition_date	1997-09-02
Structure title title	SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES
Keywords keywords	ANTITERMINATION, RNA BINDING DOMAIN, TRANSCRIPTION REGULATION; TRANSCRIPTION REGULATION
Experimental Method method	SOLUTION NMR

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	14.49 Å
Radius of gyration Rg (electron density) rg_electron	14.06 Å
Forward intensity I(0) i0	207909000.00
Molecular weight molecular_weight	127130.0 kDa
Excluded volume excluded_volume	162370 Å³
Envelope volume envelope_volume	25342 Å³
Hydration-shell volume shell_volume	13919 Å³
Envelope diameter envelope_diameter	50.1 Å
Shell Rg shell_rg	21.23 Å
Envelope Rg envelope_rg	15.91 Å
Shape Rg shape_rg	14.08 Å
Total Rg total_rg	14.24 Å
Total atoms total_atoms	18520
Residues n_residues	1100
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	43.5 Å
Rg (real space) rg_real	14.41 Å
Rg uncertainty (real space) rg_real_error	0.27 Å
I(0) (real space) i0_real	2.0790e+08
I(0) uncertainty (real space) i0_real_error	2.3570e+06
Rg (reciprocal space) rg_reciprocal	14.42 Å
I(0) (reciprocal space) i0_reciprocal	207900000.0000
Solution quality estimate total_estimate	0.8257
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	19.3 Å
Skewness Skewness skewness	0.102
Kurtosis Kurtosis kurtosis	-0.515
Angular range angular_range	— – 0.5000 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	238100.0000
Real-space data points n_real_points	80
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.918; Stabil: 0.994; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1auua_
Class class	b — All beta proteins
Fold Fold fold	b.35 — GroES-like
Superfamily Superfamily superfamily	b.35.2 — SacY-like RNA-binding domain
Family Family family	b.35.2.1 — BglG-like antiterminator proteins

Domain ID domain_id	d1auub_
Class class	b — All beta proteins
Fold Fold fold	b.35 — GroES-like
Superfamily Superfamily superfamily	b.35.2 — SacY-like RNA-binding domain
Family Family family	b.35.2.1 — BglG-like antiterminator proteins

CATH v4.4 (2 domains)

Domain ID domain_id	1auuA00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	24 — Transcription Regulation, Sacy; Chain A
Homologous superfamily homologous superfamily	10 — CAT RNA-binding domain

Domain ID domain_id	1auuB00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	24 — Transcription Regulation, Sacy; Chain A
Homologous superfamily homologous superfamily	10 — CAT RNA-binding domain

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Description description	SACY
Molecular weight formula_weight	6365.5 kDa
Source method src_method	man
Copy count entity_count	2

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	18520

来源生物 organism	Bacillus subtilis
NCBI Class编号 ncbi_taxonomy_id	1423
宿主生物 host_organism	Escherichia coli BL21(DE3)

SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES

1. Protein Identity and Related Structures Protein Identity & Related Structures

SACY

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

CATH v4.4 (2 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

fit	/app/data/saxs_computation/au/1auu/1auu.fit
pddf	/app/data/pr_computation/au/1auu/1auu.pr.out
profile	/app/data/saxs_computation/au/1auu/1auu.dat