HUMAN BETA-GLUCURONIDASE AT 2.6 A RESOLUTION

Method: X-RAY DIFFRACTION D_max: 135.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bhg
deposition_date	1996-03-04
title	HUMAN BETA-GLUCURONIDASE AT 2.6 A RESOLUTION
keywords	LYSOSOMAL ENZYME, ACID HYDROLASE, GLYCOSIDASE; GLYCOSIDASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	40.71 Å
rg_electron	40.51 Å
i0	296183000.00
molecular_weight	144000.0 kDa
excluded_volume	181110 Å³
envelope_volume	232820 Å³
shell_volume	48196 Å³
envelope_diameter	144.0 Å
shell_rg	45.53 Å
envelope_rg	40.34 Å
shape_rg	40.45 Å
total_rg	40.99 Å
total_atoms	10190
n_residues	1222
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	135.2 Å
rg_real	40.86 Å
rg_real_error	1.22 Å
i0_real	2.9620e+08
i0_real_error	4.9080e+06
rg_reciprocal	40.72 Å
i0_reciprocal	296100000.0000
total_estimate	0.8472
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	34.7 Å
skewness	0.357
kurtosis	-0.653
angular_range	— – 0.1950 Å⁻¹
current_alpha	0.0000
highest_alpha	52420000.0000
n_real_points	40
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.809; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.809; Smooth: 0.774

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	BETA-GLUCURONIDASE
formula_weight	70762.9 kDa
ec_number	3.2.1.31
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	10190

LGLQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVGPLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSVLLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVANEPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLLEQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRERYWKIANET

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Mus musculus

Entity 2 branched

description	;alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ;
formula_weight	1559.4 kDa
src_method	man
entity_count	2

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6. Citations (2)

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length_a	95.100 Å
length_b	124.400 Å
length_c	134.500 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.530 Å
resolution_low	7.000 Å
r_factor	0.2310
r_work	0.2310
r_free	0.3100

fit	/app/data/saxs_computation/bh/1bhg/1bhg.fit
pddf	/app/data/pr_computation/bh/1bhg/1bhg.pr.out
profile	/app/data/saxs_computation/bh/1bhg/1bhg.dat