SAXSdb — 1bwu

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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (AGGLUTININ)

OrganismNot specified

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain A； UniProt 177–282 Chain P； UniProt 18–123	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38784） PROTEIN (AGGLUTININ) × 1 （Q38784） MAN alpha-D-mannopyranose × 14	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278
2	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain P； UniProt 18–123	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38784） MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278
3	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 177–282	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38784） MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1BWU	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1KJ1 MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE Deposited 2001-12-04	Different construct Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 177–284（108 aa）	Not recorded	MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS, 1WEEK, pH 7.00, VAPOR DIFFUSION, HANGING DROP at 293K	Resolution 2.20 Å R-free 0.251
1KJ1 MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE Deposited 2001-12-04	Different construct Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain P 177–284（108 aa）	Not recorded	MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS, 1WEEK, pH 7.00, VAPOR DIFFUSION, HANGING DROP at 293K	Resolution 2.20 Å R-free 0.251

1 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	Q38789_ALLSA
Isoform	—
PDB entities	1, 3
Chains and sequence ranges	Author chain A； PDBConstruct 1–106； UniProt 177–282 Author chain P； PDBConstruct 1–106； UniProt 18–123

PROTEIN (AGGLUTININ)

OrganismNot specified

UniProt Q38784

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count	Chain D； UniProt 25–133 Chain Q； UniProt 25–133	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38789） PROTEIN (AGGLUTININ) × 1 （Q38789） MAN alpha-D-mannopyranose × 14	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278
2	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain Q； UniProt 25–133	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38789） MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278
3	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain D； UniProt 25–133	Not recorded	PROTEIN (AGGLUTININ) × 1 （Q38789） MAN alpha-D-mannopyranose × 7	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG8000, 5.5 MG/ML PROTEIN, 10MM MANNOSE, 20MM PBS PH 7.0, 1 WEEK, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.80 Å R-free 0.278

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	Q38784_ALLSA
Isoform	—
PDB entities	2, 4
Chains and sequence ranges	Author chain D； PDBConstruct 1–109； UniProt 25–133 Author chain Q； PDBConstruct 1–109； UniProt 25–133

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1bwu
Deposition date deposition_date	1998-09-28
Structure title title	MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE
Keywords keywords	BULB LECTIN, MANNOSE, PLANT PROTEIN; PLANT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	27.29 Å
Radius of gyration Rg (electron density) rg_electron	26.48 Å
Forward intensity I(0) i0	45394600.00
Molecular weight molecular_weight	50139.0 kDa
Excluded volume excluded_volume	61675 Å³
Envelope volume envelope_volume	77203 Å³
Hydration-shell volume shell_volume	24894 Å³
Envelope diameter envelope_diameter	89.7 Å
Shell Rg shell_rg	32.92 Å
Envelope Rg envelope_rg	26.11 Å
Shape Rg shape_rg	26.44 Å
Total Rg total_rg	27.26 Å
Total atoms total_atoms	3527
Residues n_residues	430
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	85.9 Å
Rg (real space) rg_real	27.27 Å
Rg uncertainty (real space) rg_real_error	0.62 Å
I(0) (real space) i0_real	4.5390e+07
I(0) uncertainty (real space) i0_real_error	6.4610e+05
Rg (reciprocal space) rg_reciprocal	27.28 Å
I(0) (reciprocal space) i0_reciprocal	45390000.0000
Solution quality estimate total_estimate	0.9044
Solution quality rating solution_quality	EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	28.1 Å
Skewness Skewness skewness	0.245
Kurtosis Kurtosis kurtosis	-0.552
Angular range angular_range	— – 0.2900 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	6007000.0000
Real-space data points n_real_points	59
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.958; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.892

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Description description	PROTEIN (AGGLUTININ)
Molecular weight formula_weight	11865.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	3664

RNILRNDEGLYGGQSLDVNPYHFIMQEDCNLVLYDHSTSVWASNTGILGKKGCRAVLQSDGNFVVYDAEGRSLWASHSVRGNGNYVLVLQEDGNVVIYRSDIWSTN

Entity 2 Entity 2 polymer

Description description	PROTEIN (AGGLUTININ)
Molecular weight formula_weight	12124.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

RNILTNDEGLYGGQSLDVNPYHLIMQEDCNLVLYDHSTAVWSSNTDIPGKKGCKAVLQSDGNFVVYDAEGASLWASHSVRGNGNYVLVLQEDGNVVIYRSDIWSTNTYR

Entity 3 Entity 3 polymer

Description description	PROTEIN (AGGLUTININ)
Molecular weight formula_weight	11678.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

RNILTNDEGLYAGQSLDVNPYHFIMQEDCNLVLYDHSTSVWASNTGILGKKGCKAVLQADGNFVVYDAEGRSLWASHSVRGNGNYVLVLQEDGNVVIYGSDIWSTN

Entity 4 Entity 4 polymer

Description description	PROTEIN (AGGLUTININ)
Molecular weight formula_weight	12010.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

RNILTNDEGLYAGQSLDVNPYHLIMQEDCNLVLYDHSTAVWSSNTDIPGKKGCKAVLQSDGNFVVYDAEGASLWASHSVRGNGNYVLVLQEDGNVVIYGSDIWSTNTYK

Entity 5 Entity 5 non-polymer

Description description	alpha-D-mannopyranose
Molecular weight formula_weight	180.2 kDa
Source method src_method	syn
Copy count entity_count	14

Entity 6 Entity 6 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	137

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7. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d1bwua_
Class class	b — All beta proteins
Fold Fold fold	b.78 — beta-Prism II
Superfamily Superfamily superfamily	b.78.1 — alpha-D-mannose-specific plant lectins
Family Family family	b.78.1.1 — alpha-D-mannose-specific plant lectins

Domain ID domain_id	d1bwud_
Class class	b — All beta proteins
Fold Fold fold	b.78 — beta-Prism II
Superfamily Superfamily superfamily	b.78.1 — alpha-D-mannose-specific plant lectins
Family Family family	b.78.1.1 — alpha-D-mannose-specific plant lectins

Domain ID domain_id	d1bwup_
Class class	b — All beta proteins
Fold Fold fold	b.78 — beta-Prism II
Superfamily Superfamily superfamily	b.78.1 — alpha-D-mannose-specific plant lectins
Family Family family	b.78.1.1 — alpha-D-mannose-specific plant lectins

Domain ID domain_id	d1bwuq_
Class class	b — All beta proteins
Fold Fold fold	b.78 — beta-Prism II
Superfamily Superfamily superfamily	b.78.1 — alpha-D-mannose-specific plant lectins
Family Family family	b.78.1.1 — alpha-D-mannose-specific plant lectins

CATH v4.4 (4 domains)

Domain ID domain_id	1bwuA00
Class class	2 — Mainly Beta
Architecture architecture	90 — Orthogonal Prism
Topology topology	10 — Agglutinin, subunit A
Homologous superfamily homologous superfamily	10 — Bulb-type lectin domain

Domain ID domain_id	1bwuD00
Class class	2 — Mainly Beta
Architecture architecture	90 — Orthogonal Prism
Topology topology	10 — Agglutinin, subunit A
Homologous superfamily homologous superfamily	10 — Bulb-type lectin domain

Domain ID domain_id	1bwuP00
Class class	2 — Mainly Beta
Architecture architecture	90 — Orthogonal Prism
Topology topology	10 — Agglutinin, subunit A
Homologous superfamily homologous superfamily	10 — Bulb-type lectin domain

Domain ID domain_id	1bwuQ00
Class class	2 — Mainly Beta
Architecture architecture	90 — Orthogonal Prism
Topology topology	10 — Agglutinin, subunit A
Homologous superfamily homologous superfamily	10 — Bulb-type lectin domain

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8. Citations (2)

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Unit-cell length a length_a	203.243 Å
Unit-cell length b length_b	43.780 Å
Unit-cell length c length_c	79.268 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	112.37 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	—

Resolution resolution	2.800 Å
Low-resolution limit resolution_low	9.000 Å
R factor r_factor	0.2260
R_work r_work	0.2260
R_free r_free	0.2780

fit	/app/data/saxs_computation/bw/1bwu/1bwu.fit
pddf	/app/data/pr_computation/bw/1bwu/1bwu.pr.out
profile	/app/data/saxs_computation/bw/1bwu/1bwu.dat

MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE

1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (AGGLUTININ)

State in the Current Structure

Other States of the Same Protein in the Database

PROTEIN (AGGLUTININ)

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (6)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 non-polymer

Entity 6 Entity 6 water

7. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

CATH v4.4 (4 domains)

8. Citations (2)

9. Files and Curves (10)

Files (7)

Curves (3)