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1c27

E. COLI METHIONINE AMINOPEPTIDASE:NORLEUCINE PHOSPHONATE COMPLEX

Method: X-RAY DIFFRACTION D_max: 57.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1c27
deposition_date	1999-07-22
title	E. COLI METHIONINE AMINOPEPTIDASE:NORLEUCINE PHOSPHONATE COMPLEX
keywords	PRODUCT COMPLEX, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	18.18 Å
rg_electron	17.19 Å
i0	14842300.00
molecular_weight	28624.0 kDa
excluded_volume	35616 Å³
envelope_volume	39133 Å³
shell_volume	18608 Å³
envelope_diameter	56.4 Å
shell_rg	23.79 Å
envelope_rg	17.48 Å
shape_rg	17.23 Å
total_rg	18.03 Å
total_atoms	1998
n_residues	262
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	57.2 Å
rg_real	18.07 Å
rg_real_error	0.31 Å
i0_real	1.4840e+07
i0_real_error	1.9620e+05
rg_reciprocal	18.09 Å
i0_reciprocal	14840000.0000
total_estimate	0.7252
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	22.4 Å
skewness	0.157
kurtosis	-0.426
angular_range	— – 0.4400 Å⁻¹
current_alpha	0.0000
highest_alpha	3782000.0000
n_real_points	75
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.878; Stabil: 1.000; Sysdev: 0.275; Positv: 1.000; Valcen: 0.984; Smooth: 0.980

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	METHIONINE AMINOPEPTIDASE
formula_weight	29210.6 kDa
ec_number	3.4.11.18
mutation	R175Q
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2077

AISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRICNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIKDGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAEGFSVVREYCGHGIGQGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMKDGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 2 non-polymer

description	COBALT (II) ION
formula_weight	58.9 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	SODIUM ION
formula_weight	23.0 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	(1-AMINO-PENTYL)-PHOSPHONIC ACID
formula_weight	167.1 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	79

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6. Citations (1)

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length_a	39.298 Å
length_b	67.206 Å
length_c	49.104 Å
angle_alpha	90.00 °
angle_beta	111.52 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.950 Å
resolution_low	36.600 Å
r_factor	0.1700
r_work	—
r_free	—

fit	/app/data/saxs_computation/c2/1c27/1c27.fit
pddf	/app/data/pr_computation/c2/1c27/1c27.pr.out
profile	/app/data/saxs_computation/c2/1c27/1c27.dat