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1c4o

CRYSTAL STRUCTURE OF THE DNA NUCLEOTIDE EXCISION REPAIR ENZYME UVRB FROM THERMUS THERMOPHILUS

Method: X-RAY DIFFRACTION D_max: 85.7 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1c4o
deposition_date	1999-09-14
title	CRYSTAL STRUCTURE OF THE DNA NUCLEOTIDE EXCISION REPAIR ENZYME UVRB FROM THERMUS THERMOPHILUS
keywords	DNA NUCLEOTIDE EXCISION REPAIR, UVRABC, HELICASE, HYPERTHERMOSTABLE PROTEIN, REPLICATION; REPLICATION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.20 Å
rg_electron	26.25 Å
i0	53555200.00
molecular_weight	57287.0 kDa
excluded_volume	71894 Å³
envelope_volume	89961 Å³
shell_volume	29022 Å³
envelope_diameter	88.4 Å
shell_rg	33.15 Å
envelope_rg	26.20 Å
shape_rg	26.23 Å
total_rg	27.04 Å
total_atoms	4039
n_residues	495
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	85.7 Å
rg_real	27.20 Å
rg_real_error	0.55 Å
i0_real	5.3560e+07
i0_real_error	7.7790e+05
rg_reciprocal	27.20 Å
i0_reciprocal	53560000.0000
total_estimate	0.9057
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	28.1 Å
skewness	0.304
kurtosis	-0.522
angular_range	— – 0.2900 Å⁻¹
current_alpha	0.0000
highest_alpha	13720000.0000
n_real_points	59
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.936; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.965

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	DNA NUCLEOTIDE EXCISION REPAIR ENZYME UVRB
formula_weight	76134.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4453

TFRYRGPSPKGDQPKAIAGLVEALRDGERFVTLLGATGTGKTVTMAKVIEALGRPALVLAPNKILAAQLAAEFRELFPENAVEYFISYYDYYQPEAYVPGKDLYIEKDASINPEIERLRHSTTRSLLTRRDVIVVASVSAIYGLGDPREYRARNLVVERGKPYPREVLLERLLELGYQRNDIDLSPGRFRAKGEVLEIFPAYETEPIRVELFGDEVERISQVHPVTGERLRELPGFVLFPATHYLSPEGLEEILKEIEKELWERVRYFEERGEVLYAQRLKERTLYDLEMLRVMGTCPGVENYARYFTGKAPGEPPYTLLDYFPEDFLVFLDESHVTVPQLQGMYRGDYARKKTLVDYGFRLPSALDNRPLRFEEFLERVSQVVFVSATPGPFELAHSGRVVEQIIRPTGLLDPLVRVKPTENQILDLMEGIRERAARGERTLVTVLTVRMAEELTSFLVEHGIRARYLHHELDAFKRQALIRDLRLGHYDCLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNARGEVWLYADRVSEAMQRAIEETNRRRALQEAYNLEHGITPETVRKEVRAVIRPEGYEEAPLEADLSGEDLRERIAELELAMWQAAEALDFERAARLRDEIRALEARLQGVRAPEPVPGGRKRKRR

Source

organism	Thermus thermophilus
ncbi_taxonomy_id	300852
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	octyl beta-D-glucopyranoside
formula_weight	292.4 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	4

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	336

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6. Citations (1)

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length_a	134.740 Å
length_b	134.740 Å
length_c	106.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	1.500 Å
resolution_low	41.900 Å
r_factor	0.2520
r_work	0.2520
r_free	0.2660

fit	/app/data/saxs_computation/c4/1c4o/1c4o.fit
pddf	/app/data/pr_computation/c4/1c4o/1c4o.pr.out
profile	/app/data/saxs_computation/c4/1c4o/1c4o.dat

CRYSTAL STRUCTURE OF THE DNA NUCLEOTIDE EXCISION REPAIR ENZYME UVRB FROM THERMUS THERMOPHILUS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction