← Back to search

1c8q

STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE

Method: X-RAY DIFFRACTION D_max: 83.1 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1c8q
deposition_date	2000-06-08
title	STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE
keywords	beta barrel, recombinant amylase, human saliva, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	23.87 Å
rg_electron	22.81 Å
i0	54706200.00
molecular_weight	55964.0 kDa
excluded_volume	69132 Å³
envelope_volume	77081 Å³
shell_volume	27933 Å³
envelope_diameter	83.4 Å
shell_rg	30.44 Å
envelope_rg	22.97 Å
shape_rg	22.80 Å
total_rg	23.65 Å
total_atoms	3948
n_residues	495
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	83.1 Å
rg_real	23.81 Å
rg_real_error	0.70 Å
i0_real	5.4710e+07
i0_real_error	7.3710e+05
rg_reciprocal	23.82 Å
i0_reciprocal	54710000.0000
total_estimate	0.6099
solution_quality	REASONABLE a REASONABLE solution
n_peaks	3
r_peak_primary	29.9 Å
skewness	0.333
kurtosis	-0.212
angular_range	— – 0.3350 Å⁻¹
current_alpha	0.0000
highest_alpha	10560000.0000
n_real_points	65
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.701; Stabil: 0.993; Sysdev: 0.286; Positv: 1.000; Valcen: 0.985; Smooth: 0.000

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	ALPHA-AMYLASE
formula_weight	55955.3 kDa
ec_number	3.2.1.1
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4215

QYSSNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASKHMWPGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRYFENGKDVNDWVGPPNDNGVTKEVTINPDTTCGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	267

▶

6. Citations (1)

▶

length_a	52.707 Å
length_b	74.768 Å
length_c	133.337 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	2.300 Å
resolution_low	9.990 Å
r_factor	—
r_work	0.1730
r_free	0.2160

fit	/app/data/saxs_computation/c8/1c8q/1c8q.fit
pddf	/app/data/pr_computation/c8/1c8q/1c8q.pr.out
profile	/app/data/saxs_computation/c8/1c8q/1c8q.dat

STRUCTURE SOLUTION AND REFINEMENT OF THE RECOMBINANT HUMAN SALIVARY AMYLASE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction