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1cb7

GLUTAMATE MUTASE FROM CLOSTRIDIUM COCHLEARIUM RECONSTITUTED WITH METHYL-COBALAMIN

Method: X-RAY DIFFRACTION D_max: 108.6 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cb7
deposition_date	1999-03-03
title	GLUTAMATE MUTASE FROM CLOSTRIDIUM COCHLEARIUM RECONSTITUTED WITH METHYL-COBALAMIN
keywords	GLUTAMATE MUTASE, COENZYME-B12, RADICAL REACTION, TIM-BARREL, ROSSMAN-FOLD, ISOMERASE; ISOMERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.96 Å
rg_electron	33.37 Å
i0	293567000.00
molecular_weight	139700.0 kDa
excluded_volume	175410 Å³
envelope_volume	205300 Å³
shell_volume	49867 Å³
envelope_diameter	109.5 Å
shell_rg	41.17 Å
envelope_rg	33.46 Å
shape_rg	33.38 Å
total_rg	33.88 Å
total_atoms	9810
n_residues	1240
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	108.6 Å
rg_real	33.90 Å
rg_real_error	0.59 Å
i0_real	2.9360e+08
i0_real_error	4.6870e+06
rg_reciprocal	33.94 Å
i0_reciprocal	293600000.0000
total_estimate	0.9003
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	39.3 Å
skewness	0.264
kurtosis	-0.555
angular_range	— – 0.2350 Å⁻¹
current_alpha	0.0000
highest_alpha	162600000.0000
n_real_points	48
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.923; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.936

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	PROTEIN (GLUTAMATE MUTASE)
formula_weight	14830.0 kDa
ec_number	5.4.99.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C
atom_count	10825

MEKKTIVLGVIGSDCHAVGNKILDHAFTNAGFNVVNIGVLSPQELFIKAAIETKADAILVSSLYGQGEIDCKGLRQKCDEAGLEGILLYVGGNIVVGKQHWPDVEKRFKDMGYDRVYAPGTPPEVGIADLKKDLNIE

Source

organism	Clostridium cochlearium
ncbi_taxonomy_id	1494
host_organism	Escherichia coli

Entity 2 polymer

description	PROTEIN (GLUTAMATE MUTASE)
formula_weight	53623.2 kDa
ec_number	5.4.99.1
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

MELKNKKWTDEEFHKQREEVLQQWPTGKEVDLQEAVDYLKKIPAEKNFAEKLVLAKKKGITMAQPRAGVALLDEHIELLRYLQDEGGADFLPSTIDAYTRQNRYDECENGIKESEKAGRSLLNGFPGVNFGVKGCRKVLEAVNLPLQARHGTPDSRLLAEIIHAGGWTSNEGGGISYNVPYAKNVTIEKSLLDWQYCDRLVGFYEEQGVHINREPFGPLTGTLVPPSMSNAVGITEALLAAEQGVKNITVGYGECGNMIQDIAALRCLEEQTNEYLKAYGYNDVFVTTVFHQWMGGFPQDESKAFGVIVTATTIAALAGATKVIVKTPHEAIGIPTKEANAAGIKATKMALNMLEGQRMPMSKELETEMAVIKAETKCILDKMFELGKGDLAIGTVKAFETGVMDIPFGPSKYNAGKMMPVRDNLGCVRYLEFGNVPFTEEIKNYNRERLQERAKFEGRDVSFQMVIDDIFAVGKGRLIGRPE

Source

organism	Clostridium cochlearium
ncbi_taxonomy_id	1494
host_organism	Escherichia coli

Entity 3 non-polymer

description	CO-METHYLCOBALAMIN
formula_weight	1344.4 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	D(-)-TARTARIC ACID
formula_weight	150.1 kDa
src_method	syn
entity_count	2

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	1013

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6. Citations (3)

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length_a	64.330 Å
length_b	113.140 Å
length_c	108.610 Å
angle_alpha	90.00 °
angle_beta	95.62 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	2.000 Å
resolution_low	25.000 Å
r_factor	0.1597
r_work	—
r_free	0.2185

fit	/app/data/saxs_computation/cb/1cb7/1cb7.fit
pddf	/app/data/pr_computation/cb/1cb7/1cb7.pr.out
profile	/app/data/saxs_computation/cb/1cb7/1cb7.dat

GLUTAMATE MUTASE FROM CLOSTRIDIUM COCHLEARIUM RECONSTITUTED WITH METHYL-COBALAMIN

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)