CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN

Method: X-RAY DIFFRACTION D_max: 67.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ceo
deposition_date	1995-12-04
title	CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN
keywords	GLYCOSYL HYDROLASE, CELLULASE, FAMILY A/5 OF GLYCOSYL HYDROLASES, CLOSTRIDIUM THERMOCELLUM, CELLULOSE DEGRADATION; CELLULOSE DEGRADATION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.21 Å
rg_electron	20.04 Å
i0	25122000.00
molecular_weight	39329.0 kDa
excluded_volume	49450 Å³
envelope_volume	56766 Å³
shell_volume	23188 Å³
envelope_diameter	68.6 Å
shell_rg	27.08 Å
envelope_rg	20.24 Å
shape_rg	19.99 Å
total_rg	21.08 Å
total_atoms	2782
n_residues	332
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	67.4 Å
rg_real	21.08 Å
rg_real_error	0.31 Å
i0_real	2.5120e+07
i0_real_error	3.0350e+05
rg_reciprocal	21.11 Å
i0_reciprocal	25120000.0000
total_estimate	0.8969
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.8 Å
skewness	0.174
kurtosis	-0.424
angular_range	— – 0.3750 Å⁻¹
current_alpha	0.0000
highest_alpha	5677000.0000
n_real_points	69
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.887; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.999

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (3)

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length_a	51.400 Å
length_b	84.330 Å
length_c	87.500 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.900 Å
resolution_low	10.000 Å
r_factor	0.1940
r_work	0.1940
r_free	0.2220

description	CELLULASE CELC
formula_weight	40947.4 kDa
ec_number	3.2.1.4
mutation	E140Q
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	3070

CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (3)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Clostridium thermocellum
ncbi_taxonomy_id	1515
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	288

fit	/app/data/saxs_computation/ce/1ceo/1ceo.fit
pddf	/app/data/pr_computation/ce/1ceo/1ceo.pr.out
profile	/app/data/saxs_computation/ce/1ceo/1ceo.dat