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1cf2

;THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS ;

Method: X-RAY DIFFRACTION D_max: 99.7 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cf2
deposition_date	1999-03-24
title	;THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS ;
keywords	OXYDOREDUCTASE, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.23 Å
rg_electron	32.36 Å
i0	368246000.00
molecular_weight	152350.0 kDa
excluded_volume	189800 Å³
envelope_volume	223020 Å³
shell_volume	54348 Å³
envelope_diameter	98.9 Å
shell_rg	41.65 Å
envelope_rg	32.22 Å
shape_rg	32.39 Å
total_rg	32.92 Å
total_atoms	10660
n_residues	1344
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	99.7 Å
rg_real	32.93 Å
rg_real_error	0.55 Å
i0_real	3.6820e+08
i0_real_error	5.7670e+06
rg_reciprocal	33.06 Å
i0_reciprocal	368300000.0000
total_estimate	0.9064
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	45.7 Å
skewness	0.029
kurtosis	-0.602
angular_range	— – 0.2400 Å⁻¹
current_alpha	0.0000
highest_alpha	265400000.0000
n_real_points	49
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.942; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.980; Smooth: 0.973

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN (GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE)
formula_weight	37454.8 kDa
ec_number	1.2.1.12
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	P,R,O,Q
atom_count	11620

MKAVAINGYGTVGKRVADAIAQQDDMKVIGVSKTRPDFEARMALKKGYDLYVAIPERVKLFEKAGIEVAGTVDDMLDEADIVIDCTPEGIGAKNLKMYKEKGIKAIFQGGEKHEDIGLSFNSLSNYEESYGKDYTRVVSCNTTGLCRTLKPLHDSFGIKKVRAVIVRRGADPAQVSKGPINAIIPNPPKLPSHHGPDVKTVLDINIDTMAVIVPTTLMHQHNVMVEVEETPTVDDIIDVFEDTPRVILISAEDGLTSTAEIMEYAKELGRSRNDLFEIPVWRESITVVDNEIYYMQAVHQESDIVPENVDAVRAILEMEEDKYKSINKTNKAMNILQ

Source

organism	Methanothermus fervidus
ncbi_taxonomy_id	2180
host_organism	Escherichia coli

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
formula_weight	743.4 kDa
src_method	syn
entity_count	4

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	960

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6. Citations (1)

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length_a	136.660 Å
length_b	153.280 Å
length_c	74.920 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.100 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.1940
r_free	0.2570

fit	/app/data/saxs_computation/cf/1cf2/1cf2.fit
pddf	/app/data/pr_computation/cf/1cf2/1cf2.pr.out
profile	/app/data/saxs_computation/cf/1cf2/1cf2.dat

;THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction