← Back to search

1col

REFINED STRUCTURE OF THE PORE-FORMING DOMAIN OF COLICIN A AT 2.4 ANGSTROMS RESOLUTION

Method: X-RAY DIFFRACTION D_max: 83.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1col
deposition_date	1991-07-06
title	REFINED STRUCTURE OF THE PORE-FORMING DOMAIN OF COLICIN A AT 2.4 ANGSTROMS RESOLUTION
keywords	ANTIBACTERIAL PROTEIN; ANTIBACTERIAL PROTEIN
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	25.25 Å
rg_electron	24.59 Å
i0	28617800.00
molecular_weight	42075.0 kDa
excluded_volume	53230 Å³
envelope_volume	62352 Å³
shell_volume	22569 Å³
envelope_diameter	86.1 Å
shell_rg	30.17 Å
envelope_rg	24.68 Å
shape_rg	24.57 Å
total_rg	25.36 Å
total_atoms	2956
n_residues	394
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	83.4 Å
rg_real	25.40 Å
rg_real_error	0.74 Å
i0_real	2.8620e+07
i0_real_error	4.7150e+05
rg_reciprocal	25.36 Å
i0_reciprocal	28620000.0000
total_estimate	0.8409
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.2 Å
skewness	0.515
kurtosis	-0.381
angular_range	— – 0.3150 Å⁻¹
current_alpha	0.0000
highest_alpha	9562000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.716; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.848; Smooth: 0.932

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (2)

▶

6. Citations (7)

Refined structure of the pore-forming domain of colicin A at 2.4 A resolution.

J.Mol.Biol. (1992)

PMID: 1373773 · DOI: 10.1016/0022-2836(92)90550-4 PRIMARY

Fluorescence Energy Transfer Distance Measurements Using Site-Directed Single Cysteine Mutants

J.Mol.Biol. (1991)

Colicins: Prokaryotic Killer-Pores

Experientia (1990)

Structure of the Membrane-Pore-Forming Fragment of Colicin A

Nature (1989)

The Membrane Channel-Forming Colicin A: Synthesis, Secretion, Structure, Action and Immunity

Biochim.Biophys.Acta (1988)

Gating Processes of Channels Induced by Colicin A, its C-Terminal Fragment and Colicin E1 in Planar Lipid Bilayers

Eur.Biophys.J. (1987)

Crystallization of the C-Terminal Domain of Colicin A Carrying the Voltage-Dependent Pore Activity of the Protein

J.Mol.Biol. (1986)

▶

length_a	73.000 Å
length_b	73.000 Å
length_c	171.600 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 43 21 2
int_tables_number	96
crystal_system	—

resolution	2.400 Å
resolution_low	6.000 Å
r_factor	0.1800
r_work	—
r_free	—

description	COLICIN A
formula_weight	21818.1 kDa
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	A,B
atom_count	3039

REFINED STRUCTURE OF THE PORE-FORMING DOMAIN OF COLICIN A AT 2.4 ANGSTROMS RESOLUTION

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (7)

7. Files & Curves (10)

Files (7)

Curves (3)

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	83

fit	/app/data/saxs_computation/co/1col/1col.fit
pddf	/app/data/pr_computation/co/1col/1col.pr.out
profile	/app/data/saxs_computation/co/1col/1col.dat

organism	Escherichia coli
ncbi_taxonomy_id	562