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1cr4

CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP

Method: X-RAY DIFFRACTION D_max: 74.1 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cr4
deposition_date	1999-08-12
title	CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP
keywords	RECA-TYPE PROTEIN FOLD, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	19.77 Å
rg_electron	18.61 Å
i0	13393300.00
molecular_weight	26832.0 kDa
excluded_volume	33374 Å³
envelope_volume	40089 Å³
shell_volume	18428 Å³
envelope_diameter	76.0 Å
shell_rg	24.72 Å
envelope_rg	19.34 Å
shape_rg	18.61 Å
total_rg	19.52 Å
total_atoms	1875
n_residues	243
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	74.1 Å
rg_real	19.78 Å
rg_real_error	0.56 Å
i0_real	1.3390e+07
i0_real_error	1.7680e+05
rg_reciprocal	19.78 Å
i0_reciprocal	13390000.0000
total_estimate	0.7330
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	24.0 Å
skewness	0.458
kurtosis	0.222
angular_range	— – 0.4000 Å⁻¹
current_alpha	0.0000
highest_alpha	2100000.0000
n_real_points	72
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.546; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.888; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	DNA PRIMASE/HELICASE
formula_weight	32832.0 kDa
ec_number	2.7.7.-
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1890

MRERIREHLSSEESVGLLFSGCTGINDKTLGARGGEVIMVTSGSGMGKSTFVRQQALQWGTAMGKKVGLAMLEESVEETAEDLIGLHNRVRLRQSDSLKREIIENGKFDQWFDELFGNDTFHLYDSFAEAETDRLLAKLAYMRSGLGCDVIILDHISIVVSASGESDERKMIDNLMTKLKGFAKSTGVVLVVICHLKNPDKGKAHEEGRPVSITDLRGSGALRQLSDTIIALERNQQGDMPNLVLVRILKCRFTGDTGIAGYMEYNKETGWLEPSSYSGEEESHSESTDWSNDTDF

Source

organism	Enterobacteria phage T7
ncbi_taxonomy_id	10760
host_organism	Escherichia coli

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	THYMIDINE-5'-DIPHOSPHATE
formula_weight	402.2 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	15

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6. Citations (1)

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length_a	80.784 Å
length_b	80.784 Å
length_c	85.852 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 61
int_tables_number	169
crystal_system	—

resolution	2.500 Å
resolution_low	20.000 Å
r_factor	0.2510
r_work	0.2420
r_free	0.2870

fit	/app/data/saxs_computation/cr/1cr4/1cr4.fit
pddf	/app/data/pr_computation/cr/1cr4/1cr4.pr.out
profile	/app/data/saxs_computation/cr/1cr4/1cr4.dat

CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE 4 PROTEIN OF BACTERIOPHAGE T7: COMPLEX WITH DTDP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction