CRYSTAL STRUCTURE OF AMYLOMALTASE FROM THERMUS AQUATICUS, A GLYCOSYLTRANSFERASE CATALYSING THE PRODUCTION OF LARGE CYCLIC GLUCANS

Method: X-RAY DIFFRACTION D_max: 79.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cwy
deposition_date	1999-08-27
title	CRYSTAL STRUCTURE OF AMYLOMALTASE FROM THERMUS AQUATICUS, A GLYCOSYLTRANSFERASE CATALYSING THE PRODUCTION OF LARGE CYCLIC GLUCANS
keywords	(BETA, ALPHA)8 BARREL, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.41 Å
rg_electron	23.07 Å
i0	51194600.00
molecular_weight	57210.0 kDa
excluded_volume	72117 Å³
envelope_volume	83790 Å³
shell_volume	29591 Å³
envelope_diameter	83.8 Å
shell_rg	30.84 Å
envelope_rg	23.16 Å
shape_rg	23.04 Å
total_rg	24.06 Å
total_atoms	4064
n_residues	500
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	79.3 Å
rg_real	24.27 Å
rg_real_error	0.45 Å
i0_real	5.1190e+07
i0_real_error	6.8920e+05
rg_reciprocal	24.31 Å
i0_reciprocal	51200000.0000
total_estimate	0.8890
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	30.2 Å
skewness	0.193
kurtosis	-0.403
angular_range	— – 0.3250 Å⁻¹
current_alpha	0.0000
highest_alpha	11820000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.856; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.985

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	155.700 Å
length_b	155.700 Å
length_c	64.200 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 64
int_tables_number	172
crystal_system	—

resolution	2.000 Å
resolution_low	500.000 Å
r_factor	0.1900
r_work	0.1900
r_free	0.2220

description	AMYLOMALTASE
formula_weight	57293.0 kDa
ec_number	2.4.1.25
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	4803

CRYSTAL STRUCTURE OF AMYLOMALTASE FROM THERMUS AQUATICUS, A GLYCOSYLTRANSFERASE CATALYSING THE PRODUCTION OF LARGE CYCLIC GLUCANS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Thermus aquaticus
ncbi_taxonomy_id	271
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	739

fit	/app/data/saxs_computation/cw/1cwy/1cwy.fit
pddf	/app/data/pr_computation/cw/1cwy/1cwy.pr.out
profile	/app/data/saxs_computation/cw/1cwy/1cwy.dat