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1cx6

T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE

Method: X-RAY DIFFRACTION D_max: 59.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cx6
deposition_date	1999-08-28
title	T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE
keywords	HYDROLASE (O-GLYCOSYL), T4 LYSOZYME, SELENOMETHIONINE CORE MUTANT, PROTEIN ENGINEERING, PROTEIN FOLDING, HYDROLASE; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	17.06 Å
rg_electron	16.39 Å
i0	7902350.00
molecular_weight	19257.0 kDa
excluded_volume	23335 Å³
envelope_volume	26710 Å³
shell_volume	14147 Å³
envelope_diameter	58.6 Å
shell_rg	21.79 Å
envelope_rg	16.55 Å
shape_rg	16.46 Å
total_rg	17.08 Å
total_atoms	1299
n_residues	150
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	59.3 Å
rg_real	17.06 Å
rg_real_error	0.44 Å
i0_real	7.9020e+06
i0_real_error	9.9560e+04
rg_reciprocal	17.06 Å
i0_reciprocal	7902000.0000
total_estimate	0.7816
solution_quality	GOOD a GOOD solution
n_peaks	3
r_peak_primary	17.9 Å
skewness	0.385
kurtosis	-0.252
angular_range	— – 0.4650 Å⁻¹
current_alpha	0.0000
highest_alpha	2467000.0000
n_real_points	77
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.746; Stabil: 0.997; Sysdev: 1.000; Positv: 1.000; Valcen: 0.927; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	LYSOZYME
formula_weight	19283.4 kDa
ec_number	3.2.1.17
mutation	C54T, L84(MSE), L91(MSE), C97A, L99(MSE), L118(MSE), L121(MSE), L133(MSE), F153(MSE)
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1405

MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKMKPVYDSMDAVRRAAMINMVFQMGETGVAGFTNSMRMMQQKRWDEAAVNMAKSRWYNQTPNRAKRVITTMRTGTWDAYKNL

Source

organism	Enterobacteria phage T4
ncbi_taxonomy_id	10665
host_organism	Escherichia coli

Entity 2 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	2-HYDROXYETHYL DISULFIDE
formula_weight	154.3 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	106

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6. Citations (3)

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length_a	61.400 Å
length_b	61.400 Å
length_c	96.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.010 Å
resolution_low	30.000 Å
r_factor	0.1580
r_work	0.1580
r_free	—

fit	/app/data/saxs_computation/cx/1cx6/1cx6.fit
pddf	/app/data/pr_computation/cx/1cx6/1cx6.pr.out
profile	/app/data/saxs_computation/cx/1cx6/1cx6.dat

T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction