SAXSdb — 1cz4

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1. Protein Identity and Related Structures Protein Identity & Related Structures

VCP-LIKE ATPASE

Thermoplasma acidophilum

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 1–183	Fragment：N-TERMINAL DOMAIN: M1 TO E183 FOLLOWED BY A DIGLYCINE SPACER	No other associated polymer	SOLUTION NMR NMR measurement conditions：pH 5.9；320 K；Ionic strength (raw mmCIF value) 80 mM；Pressure AMBIENT NMR measurement conditions：pH 5.9；320 K；Ionic strength (raw mmCIF value) 80 mM；Pressure AMBIENT NMR sample composition：1.4 MM VAT-NU-15N,13C; 40 MM PHOSPHATE BUFFER NA; 90% H2O, 10% D2O NMR sample composition：1.2 MM VAT-N U-15N; 40 MM PHOSPHATE BUFFER NA; 90% H2O, 10% D2O	Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1CZ4	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1CZ5 NMR STRUCTURE OF VAT-N: THE N-TERMINAL DOMAIN OF VAT (VCP-LIKE ATPASE OF THERMOPLASMA) Deposited 1999-09-01	Different experimental conditions	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–183（183 aa） Fragment：N-TERMINAL DOMAIN: M1 TO E183 FOLLOWED BY A DIGLYCINE SPACER	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5.9；320 K；Ionic strength (raw mmCIF value) 80 mM；Pressure AMBIENT NMR measurement conditions pH 5.9；320 K；Ionic strength (raw mmCIF value) 80 mM；Pressure AMBIENT NMR sample composition 1.4 MM VAT-N U-15N,13C; 40 MM PHOSPHATE BUFFER NA; 90% H2O, 10% D2O NMR sample composition 1.2 MM VAT-N U-15N; 40 MM PHOSPHATE BUFFER NA; 90% H2O, 10% D2O	Resolution not provided
5G4F Structure of the ADP-bound VAT complex Deposited 2016-05-12	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 1–726（726 aa） Chain B 1–726（726 aa） Chain C 1–726（726 aa） Chain D 1–726（726 aa） Chain E 1–726（726 aa） Chain P 1–726（726 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES, 100 MM NACL, 5MM ADP；pH 7.5；50 MM HEPES, 100 MM NACL, 5MM ADP cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；VITRIFICATION 1 -- CRYOGEN- ETHANE-PROPANE MIXTURE, HUMIDITY- 100, INSTRUMENT- FEI VITROBOT MARK III, METHOD- BLOT FOR 4 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE HELD AT 4 DEGREES CELSIUS BEFORE FREEZING	Resolution 7.00 Å
5G4G Structure of the ATPgS-bound VAT complex Deposited 2016-05-12	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 6–726（721 aa） Chain B 6–726（721 aa） Chain C 6–726（721 aa） Chain D 6–726（721 aa） Chain E 6–726（721 aa） Chain F 6–726（721 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer 50 MM HEPES, 100 MM NACL, 5MM ATPGS；pH 7.5；50 MM HEPES, 100 MM NACL, 5MM ATPGS cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；VITRIFICATION 1 -- CRYOGEN- ETHANE-PROPANE MIXTURE, HUMIDITY- 100, INSTRUMENT- FEI VITROBOT MARK III, METHOD- BLOT FOR 4 SECONDS BEFORE PLUNGING,	Resolution 7.80 Å
5VC7 VCP like ATPase from T. acidophilum (VAT) - conformation 1 Deposited 2017-03-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 183–745（563 aa） Fragment：UNP residues 183-745 Chain C 183–745（563 aa） Fragment：UNP residues 183-745 Chain D 183–745（563 aa） Fragment：UNP residues 183-745 Chain E 183–745（563 aa） Fragment：UNP residues 183-745 Chain F 183–745（563 aa） Fragment：UNP residues 183-745 Chain G 183–745（563 aa） Fragment：UNP residues 183-745	Not recorded	ATP ADENOSINE-5'-TRIPHOSPHATE × 12	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；Blot for 4 seconds before plunging	Resolution 3.90 Å
5VCA VCP like ATPase from T. acidophilum (VAT)-Substrate bound conformation Deposited 2017-03-31	Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain M 183–745（563 aa） Fragment：UNP residues 183-745 Chain N 183–745（563 aa） Fragment：UNP residues 183-745 Chain O 183–745（563 aa） Fragment：UNP residues 183-745 Chain P 183–745（563 aa） Fragment：UNP residues 183-745 Chain Q 183–745（563 aa） Fragment：UNP residues 183-745 Chain R 183–745（563 aa） Fragment：UNP residues 183-745	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；Blot for 4 seconds before plunging	Resolution 4.80 Å
7DBO DPBB domain of VCP-like ATPase from Thermoplasma acidophilum Deposited 2020-10-21	Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–91（91 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100mM citrate / phosphate pH5.0, 1.4M Ammonium sulfate	Resolution 1.90 Å R-free 0.264
7DBO DPBB domain of VCP-like ATPase from Thermoplasma acidophilum Deposited 2020-10-21	Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–91（91 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；100mM citrate / phosphate pH5.0, 1.4M Ammonium sulfate	Resolution 1.90 Å R-free 0.264

6 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	VAT_THEAC
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–183； UniProt 1–183

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1cz4
Deposition date deposition_date	1999-09-01
Structure title title	NMR STRUCTURE OF VAT-N: THE N-TERMINAL DOMAIN OF VAT (VCP-LIKE ATPASE OF THERMOPLASMA)
Keywords keywords	DOUBLE-PSI BETA-BARREL, BETA-CLAM, SUBSTRATE RECOGNITION DOMAIN, HYDROLASE; HYDROLASE
Experimental Method method	SOLUTION NMR

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	18.25 Å
Radius of gyration Rg (electron density) rg_electron	17.13 Å
Forward intensity I(0) i0	8250190.00
Molecular weight molecular_weight	20619.0 kDa
Excluded volume excluded_volume	25813 Å³
Envelope volume envelope_volume	31564 Å³
Hydration-shell volume shell_volume	15696 Å³
Envelope diameter envelope_diameter	59.8 Å
Shell Rg shell_rg	22.88 Å
Envelope Rg envelope_rg	17.45 Å
Shape Rg shape_rg	17.12 Å
Total Rg total_rg	18.14 Å
Total atoms total_atoms	2961
Residues n_residues	185
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	60.6 Å
Rg (real space) rg_real	18.20 Å
Rg uncertainty (real space) rg_real_error	0.43 Å
I(0) (real space) i0_real	8.2500e+06
I(0) uncertainty (real space) i0_real_error	1.1360e+05
Rg (reciprocal space) rg_reciprocal	18.21 Å
I(0) (reciprocal space) i0_reciprocal	8250000.0000
Solution quality estimate total_estimate	0.8826
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	22.1 Å
Skewness Skewness skewness	0.293
Kurtosis Kurtosis kurtosis	-0.305
Angular range angular_range	— – 0.4350 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	2251000.0000
Real-space data points n_real_points	75
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.829; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.982

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1cz4a1
Class class	b — All beta proteins
Fold Fold fold	b.52 — Double psi beta-barrel
Superfamily Superfamily superfamily	b.52.2 — ADC-like
Family Family family	b.52.2.3 — Cdc48 N-terminal domain-like

Domain ID domain_id	d1cz4a2
Class class	d — Alpha and beta proteins (a+b)
Fold Fold fold	d.31 — Cdc48 domain 2-like
Superfamily Superfamily superfamily	d.31.1 — Cdc48 domain 2-like
Family Family family	d.31.1.1 — Cdc48 domain 2-like

CATH v4.4 (2 domains)

Domain ID domain_id	1cz4A01
Class class	2 — Mainly Beta
Architecture architecture	40 — Beta Barrel
Topology topology	40 — Barwin-like endoglucanases
Homologous superfamily homologous superfamily	20 —

Domain ID domain_id	1cz4A02
Class class	3 — Alpha Beta
Architecture architecture	10 — Roll
Topology topology	330 — Vcp-like ATPase; Chain A, domain 2
Homologous superfamily homologous superfamily	10 —

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Description description	VCP-LIKE ATPASE
Molecular weight formula_weight	20649.8 kDa
Source method src_method	man
Copy count entity_count	1

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	2961

来源生物 organism	Thermoplasma acidophilum
NCBI Class编号 ncbi_taxonomy_id	2303
宿主生物 host_organism	Escherichia coli

NMR STRUCTURE OF VAT-N: THE N-TERMINAL DOMAIN OF VAT (VCP-LIKE ATPASE OF THERMOPLASMA)

1. Protein Identity and Related Structures Protein Identity & Related Structures

VCP-LIKE ATPASE

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

CATH v4.4 (2 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

fit	/app/data/saxs_computation/cz/1cz4/1cz4.fit
pddf	/app/data/pr_computation/cz/1cz4/1cz4.pr.out
profile	/app/data/saxs_computation/cz/1cz4/1cz4.dat