← Back to search
1cza

MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP

Method: X-RAY DIFFRACTION Dmax: 130.5 Å Quality: REASONABLE
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

HEXOKINASE TYPE I

Homo sapiens

UniProt P19367

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain N； UniProt 1–917 Mutation：E280A, R283A, G284Y GLC alpha-D-glucopyranose × 2 G6P 6-O-phosphono-alpha-D-glucopyranose × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：EVAPORATION；pH 6.5；298 K；PEG 8000, SODIUM ACETATE, MES, ADP, ALUMINUM NITRATE, SODIUM FLUORIDE, MAGNESIUM ACETATE, GLUCOSE, pH 6.5, EVAPORATION, temperature 298.0K Resolution 1.90 Å R-free 0.258

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1CZA Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1DGK MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I WITH GLUCOSE AND ADP IN THE ACTIVE SITE Deposited 1999-11-24 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain N 1–917（917 aa）
Mutation：E280A, R283A, G284Y, T536A GLC alpha-D-glucopyranose × 2 PO4 PHOSPHATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；PEG 8000, SODIUM ACETATE, MES, ADP, GLUCOSE, GLUCOSE-6-PHOSPHATE. GROWN CRYSTALS WERE SOAKED WITH THE SAME BUFFER, BUT W/O GLUCOSE-6-PHOSPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.80 Å R-free 0.308
1HKB CRYSTAL STRUCTURE OF RECOMBINANT HUMAN BRAIN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND GLUCOSE-6-PHOSPHATE Deposited 1997-12-01 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 2 G6P 6-O-phosphono-alpha-D-glucopyranose × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.6；HEXOKINASE WAS CRYSTALLIZED BY MIXING 20 MG/ML OF PROTEIN SOLUTION WITH 6% PEG 8000, 0.1 M NA CITRATE, PH 5.6, 0.1 M NA ACETATE, 20 MM GLUCOSE-6-PHOSPHATE, AND PRESUMABLE TRACE AMOUNT OF GLUCOSE LEFT AFTER PURIFICATION; THEN SOAKED WITH 10 MM G6P AND 5 MM GLUCOSE.
Resolution 2.80 Å R-free 0.270
1HKB CRYSTAL STRUCTURE OF RECOMBINANT HUMAN BRAIN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND GLUCOSE-6-PHOSPHATE Deposited 1997-12-01 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 2 G6P 6-O-phosphono-alpha-D-glucopyranose × 2 CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.6；HEXOKINASE WAS CRYSTALLIZED BY MIXING 20 MG/ML OF PROTEIN SOLUTION WITH 6% PEG 8000, 0.1 M NA CITRATE, PH 5.6, 0.1 M NA ACETATE, 20 MM GLUCOSE-6-PHOSPHATE, AND PRESUMABLE TRACE AMOUNT OF GLUCOSE LEFT AFTER PURIFICATION; THEN SOAKED WITH 10 MM G6P AND 5 MM GLUCOSE.
Resolution 2.80 Å R-free 0.270
1HKC RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE Deposited 1998-07-01 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 2 PO4 PHOSPHATE ION × 4 K POTASSIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；HEXOKINASE WAS CRYSTALLIZED BY HANGING DROP METHOD AT ROOM TEMPERATURE BY MIXING 20 MG/ ML OF PROTEIN IN 25 MM POTASSIUM PHOSPHATE WITH A PRECIPITANT SOLUTION CONTAINING 5% PEG 8000 AND 0.1 M NA CACODYLATE, PH 6.5. PROTEIN SAMPLES CONTAINED A TRACE AMOUNT OF GLUCOSE LEFT AFTER PURIFICATION.
Resolution 2.80 Å R-free 0.241
1QHA HUMAN HEXOKINASE TYPE I COMPLEXED WITH ATP ANALOGUE AMP-PNP Deposited 1999-05-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Chain B 1–917（917 aa）
Not recorded GLC alpha-D-glucopyranose × 4 G6P 6-O-phosphono-alpha-D-glucopyranose × 4 MG MAGNESIUM ION × 6 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；pH 6.5
Resolution 2.25 Å R-free 0.279
4F9O Crystal Structure of recombinant human Hexokinase type I with 2-deoxy-Glucose 6-Phosphate Deposited 2012-05-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–914（914 aa）
Not recorded BGC beta-D-glucopyranose × 2 0NZ 2-deoxy-6-O-phosphono-beta-D-glucopyranose × 2 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.65 Å R-free 0.258
4F9O Crystal Structure of recombinant human Hexokinase type I with 2-deoxy-Glucose 6-Phosphate Deposited 2012-05-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–914（914 aa）
Not recorded BGC beta-D-glucopyranose × 2 0NZ 2-deoxy-6-O-phosphono-beta-D-glucopyranose × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.65 Å R-free 0.258
4FOE Crystal Structure of recombinant human Hexokinase type I with Mannose 6-Phosphate Deposited 2012-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Chain B 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 4 M6D 6-O-phosphono-beta-D-mannopyranose × 4 NA SODIUM ION × 4 CIT CITRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.70 Å R-free 0.254
4FOI Crystal Structure of recombinant human Hexokinase type I mutant D413N with Glucose 1,6-bisphosphate Deposited 2012-06-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Chain B 1–917（917 aa）
Mutation：D413N Mutation：D413N BGC beta-D-glucopyranose × 4 G16 1,6-di-O-phosphono-alpha-D-glucopyranose × 4 NA SODIUM ION × 4 CIT CITRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.40 Å R-free 0.273
4FOI Crystal Structure of recombinant human Hexokinase type I mutant D413N with Glucose 1,6-bisphosphate Deposited 2012-06-20 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–917（917 aa）
Mutation：D413N BGC beta-D-glucopyranose × 2 G16 1,6-di-O-phosphono-alpha-D-glucopyranose × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.40 Å R-free 0.273
4FOI Crystal Structure of recombinant human Hexokinase type I mutant D413N with Glucose 1,6-bisphosphate Deposited 2012-06-20 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–917（917 aa）
Mutation：D413N BGC beta-D-glucopyranose × 2 G16 1,6-di-O-phosphono-alpha-D-glucopyranose × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.40 Å R-free 0.273
4FPA Crystal Structure of recombinant human Hexokinase type I mutant D413N Glucose 6-Phosphate Deposited 2012-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Chain B 1–917（917 aa）
Mutation：D413N Mutation：D413N BGC beta-D-glucopyranose × 4 BG6 6-O-phosphono-beta-D-glucopyranose × 4 NA SODIUM ION × 4 CIT CITRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.48 Å R-free 0.256
4FPA Crystal Structure of recombinant human Hexokinase type I mutant D413N Glucose 6-Phosphate Deposited 2012-06-21 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–917（917 aa）
Mutation：D413N BGC beta-D-glucopyranose × 2 BG6 6-O-phosphono-beta-D-glucopyranose × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.48 Å R-free 0.256
4FPA Crystal Structure of recombinant human Hexokinase type I mutant D413N Glucose 6-Phosphate Deposited 2012-06-21 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–917（917 aa）
Mutation：D413N BGC beta-D-glucopyranose × 2 BG6 6-O-phosphono-beta-D-glucopyranose × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 2.48 Å R-free 0.256
4FPB Crystal Structure of Recombinant Human Hexokinase Type I with 1,5-Anhydroglucitol 6-Phosphate Deposited 2012-06-21 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 1–917（917 aa）
Chain B 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 4 0WK 1,5-anhydro-6-O-phosphono-D-glucitol × 4 NA SODIUM ION × 4 CIT CITRIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 3.00 Å R-free 0.256
4FPB Crystal Structure of Recombinant Human Hexokinase Type I with 1,5-Anhydroglucitol 6-Phosphate Deposited 2012-06-21 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 2 0WK 1,5-anhydro-6-O-phosphono-D-glucitol × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 3.00 Å R-free 0.256
4FPB Crystal Structure of Recombinant Human Hexokinase Type I with 1,5-Anhydroglucitol 6-Phosphate Deposited 2012-06-21 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–917（917 aa）
Not recorded BGC beta-D-glucopyranose × 2 0WK 1,5-anhydro-6-O-phosphono-D-glucitol × 2 NA SODIUM ION × 2 CIT CITRIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions hanging drop；pH 6；277 K；PEG 4000, PEG 8000, sodium acetate, sodium citrate, pH 6.0, hanging drop, temperature 277K
Resolution 3.00 Å R-free 0.256

9 other PDB entries and 17 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name HXK1_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain N； PDBConstruct 1–917； UniProt 1–917

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1cza

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1cza
Download Download
▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id1cza
Deposition date deposition_date1999-09-01
Structure title titleMUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP
Keywords keywordsSTRUCTURALLY HOMOLOGOUS DOMAINS, TRANSFERASE; TRANSFERASE
Experimental Method methodX-RAY DIFFRACTION
▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier39.29 Å
Radius of gyration Rg (electron density) rg_electron39.56 Å
Forward intensity I(0) i0162545000.00
Molecular weight molecular_weight101600.0 kDa
Excluded volume excluded_volume126510 ų
Envelope volume envelope_volume164350 ų
Hydration-shell volume shell_volume36648 ų
Envelope diameter envelope_diameter140.8 Å
Shell Rg shell_rg42.50 Å
Envelope Rg envelope_rg39.24 Å
Shape Rg shape_rg39.55 Å
Total Rg total_rg39.74 Å
Total atoms total_atoms7104
Residues n_residues898
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax130.5 Å
Rg (real space) rg_real39.86 Å
Rg uncertainty (real space) rg_real_error1.51 Å
I(0) (real space) i0_real1.6250e+08
I(0) uncertainty (real space) i0_real_error3.0770e+06
Rg (reciprocal space) rg_reciprocal39.51 Å
I(0) (reciprocal space) i0_reciprocal162500000.0000
Solution quality estimate total_estimate0.7428
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary29.4 Å
Skewness Skewness skewness0.491
Kurtosis Kurtosis kurtosis-0.609
Angular range angular_range— – 0.2000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha23460000.0000
Real-space data points n_real_points41
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.605; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.536; Smooth: 0.300
▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (5)

▼

7. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_idd1czan1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.3 — Hexokinase
Domain ID domain_idd1czan2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.3 — Hexokinase
Domain ID domain_idd1czan3
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.3 — Hexokinase
Domain ID domain_idd1czan4
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.55 — Ribonuclease H-like motif
Superfamily Superfamily superfamilyc.55.1 — Actin-like ATPase domain
Family Family familyc.55.1.3 — Hexokinase

CATH v4.4 (4 domains)

Domain ID domain_id1czaN01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id1czaN02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology367 — Hexokinase; domain 1
Homologous superfamily homologous superfamily20 —
Domain ID domain_id1czaN03
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology420 — Nucleotidyltransferase; domain 5
Homologous superfamily homologous superfamily40 — ATPase, nucleotide binding domain
Domain ID domain_id1czaN04
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology367 — Hexokinase; domain 1
Homologous superfamily homologous superfamily20 —
▶

8. Citations (3)

▶

9. Files and Curves (10)