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1djw

PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-2-METHYLENE-1,2-CYCLIC-MONOPHOSPHONATE

Method: X-RAY DIFFRACTION D_max: 149.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1djw
deposition_date	1996-08-24
title	PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-2-METHYLENE-1,2-CYCLIC-MONOPHOSPHONATE
keywords	PHOSPHORIC DIESTER HYDROLASE, HYDROLASE, LIPID DEGRADATION, TRANSDUCER, CALCIUM-BINDING, PHOSPHOLIPASE C, PHOSPHOINOSITIDE-SPECIFIC; LIPID DEGRADATION
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	41.60 Å
rg_electron	41.91 Å
i0	229516000.00
molecular_weight	121920.0 kDa
excluded_volume	151890 Å³
envelope_volume	198590 Å³
shell_volume	42724 Å³
envelope_diameter	158.0 Å
shell_rg	42.44 Å
envelope_rg	42.10 Å
shape_rg	41.89 Å
total_rg	42.01 Å
total_atoms	8566
n_residues	1074
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	149.8 Å
rg_real	42.05 Å
rg_real_error	2.04 Å
i0_real	2.2950e+08
i0_real_error	4.8110e+06
rg_reciprocal	41.61 Å
i0_reciprocal	229400000.0000
total_estimate	0.8005
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	35.5 Å
skewness	0.595
kurtosis	-0.211
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0000
highest_alpha	25000000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.638; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.692; Smooth: 0.797

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C, ISOZYME DELTA1
formula_weight	70574.5 kDa
ec_number	3.1.4.11
mutation	DELTA(1-132) DELETION VARIANT
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	9318

GSMDQRQKLQHWIHSCLRKADKNKDNKMNFKELKDFLKELNIQVDDGYARKIFRECDHSQTDSLEDEEIETFYKMLTQRAEIDRAFEEAAGSAETLSVERLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMDQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMARHLRAILGPILLDQPLDGVTTSLPSPEQLKGKILLKGKKLGGLLPAGGENGSEATDVSDEVEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMIIYCKSVHFGGFSSPGTSGQAFYEMASFSESRALRLLQESGNGFVRHNVSCLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPNTTFNSRALTQGPWWRPERLRVRIISGQQLPKVNKNKNSIVDPKVIVEIHGVGRDTGSRQTAVITNNGFNPRWDMEFEFEVTVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDQHPSATLFVKISIQD

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	6

Entity 3 non-polymer

description	ACETATE ION
formula_weight	59.0 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	INOSITOL-2-METHYLENE-1,2-CYCLIC-MONOPHOSPHATE
formula_weight	240.1 kDa
src_method	syn
entity_count	2

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	752

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6. Citations (2)

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length_a	397.030 Å
length_b	397.030 Å
length_c	397.030 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	192

space_group	F 41 3 2
int_tables_number	210
crystal_system	—

resolution	2.450 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.2170
r_free	0.2700

fit	/app/data/saxs_computation/dj/1djw/1djw.fit
pddf	/app/data/pr_computation/dj/1djw/1djw.pr.out
profile	/app/data/saxs_computation/dj/1djw/1djw.dat