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1dkg

CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK

Method: X-RAY DIFFRACTION D_max: 114.7 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dkg
deposition_date	1997-02-13
title	CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK
keywords	HSP70, GRPE, MOLECULAR CHAPERONE, NUCLEOTIDE EXCHANGE FACTOR, COILED-COIL, COMPLEX (HSP24-HSP70), COMPLEX (HSP24-HSP70) complex; COMPLEX (HSP24/HSP70)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.85 Å
rg_electron	31.31 Å
i0	87627300.00
molecular_weight	73482.0 kDa
excluded_volume	92059 Å³
envelope_volume	123580 Å³
shell_volume	34712 Å³
envelope_diameter	123.1 Å
shell_rg	36.06 Å
envelope_rg	32.06 Å
shape_rg	31.31 Å
total_rg	31.75 Å
total_atoms	5156
n_residues	685
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	114.7 Å
rg_real	32.07 Å
rg_real_error	1.38 Å
i0_real	8.7630e+07
i0_real_error	1.6240e+06
rg_reciprocal	31.97 Å
i0_reciprocal	87620000.0000
total_estimate	0.6265
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	36.7 Å
skewness	0.584
kurtosis	0.184
angular_range	— – 0.2500 Å⁻¹
current_alpha	0.0000
highest_alpha	9441000.0000
n_real_points	51
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.702; Stabil: 1.000; Sysdev: 0.062; Positv: 1.000; Valcen: 0.943; Smooth: 0.904

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	NUCLEOTIDE EXCHANGE FACTOR GRPE
formula_weight	21882.9 kDa
mutation	CHAIN A, B, G122D
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	5183

MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKVANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVADKANPDMSAMVEDIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 2 polymer

description	MOLECULAR CHAPERONE DNAK
formula_weight	41572.1 kDa
mutation	CHAIN D, P319L
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIELLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLT

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	27

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6. Citations (1)

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length_a	149.430 Å
length_b	149.430 Å
length_c	49.030 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 41
int_tables_number	76
crystal_system	—

resolution	2.800 Å
resolution_low	30.000 Å
r_factor	0.2230
r_work	0.2230
r_free	0.3170

fit	/app/data/saxs_computation/dk/1dkg/1dkg.fit
pddf	/app/data/pr_computation/dk/1dkg/1dkg.pr.out
profile	/app/data/saxs_computation/dk/1dkg/1dkg.dat

CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)