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1dsy

C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE

Method: X-RAY DIFFRACTION D_max: 53.1 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dsy
deposition_date	2000-01-10
title	C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE
keywords	CALCIUM++, PHOSPHOLIPID BINDING PROTEIN, CALCIUM-BINDING PROTEIN, PHOSPHATIDYLSERINE, PROTEIN KINASE C, TRANSFERASE; TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	16.07 Å
rg_electron	14.93 Å
i0	5113490.00
molecular_weight	16268.0 kDa
excluded_volume	20346 Å³
envelope_volume	22607 Å³
shell_volume	13034 Å³
envelope_diameter	51.5 Å
shell_rg	20.52 Å
envelope_rg	15.28 Å
shape_rg	14.87 Å
total_rg	16.14 Å
total_atoms	1141
n_residues	137
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	53.1 Å
rg_real	16.00 Å
rg_real_error	0.33 Å
i0_real	5.1130e+06
i0_real_error	6.2610e+04
rg_reciprocal	16.01 Å
i0_reciprocal	5114000.0000
total_estimate	0.8867
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	20.2 Å
skewness	0.225
kurtosis	-0.327
angular_range	— – 0.4950 Å⁻¹
current_alpha	0.0000
highest_alpha	758900.0000
n_real_points	80
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.847; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.986

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	PROTEIN KINASE C, ALPHA TYPE
formula_weight	16240.5 kDa
ec_number	2.7.1.-
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1180

HTEKRGRIYLKAEVTDEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEG

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	CALCIUM ION
formula_weight	40.1 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE
formula_weight	455.4 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	39

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6. Citations (1)

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length_a	58.800 Å
length_b	58.800 Å
length_c	91.700 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.600 Å
resolution_low	18.000 Å
r_factor	—
r_work	0.1947
r_free	0.2327

fit	/app/data/saxs_computation/ds/1dsy/1dsy.fit
pddf	/app/data/pr_computation/ds/1dsy/1dsy.pr.out
profile	/app/data/saxs_computation/ds/1dsy/1dsy.dat