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1dym

Humicola insolens Endocellulase Cel7B (EG 1) E197A Mutant

Method: X-RAY DIFFRACTION D_max: 71.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dym
deposition_date	2000-02-03
title	Humicola insolens Endocellulase Cel7B (EG 1) E197A Mutant
keywords	ENDOGLUCANASE, CELLULOSE DEGRADATION, HYDROLASE, GLYCOSIDASE, GLYCOPROTEIN, GLYCOSYNTHASE, GLYCOSIDE HYDROLASE FAMILY 7; HYDROLASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	21.88 Å
rg_electron	20.81 Å
i0	35670200.00
molecular_weight	44388.0 kDa
excluded_volume	54775 Å³
envelope_volume	63631 Å³
shell_volume	24799 Å³
envelope_diameter	74.3 Å
shell_rg	28.30 Å
envelope_rg	21.24 Å
shape_rg	20.79 Å
total_rg	21.75 Å
total_atoms	3111
n_residues	397
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	71.6 Å
rg_real	21.75 Å
rg_real_error	0.42 Å
i0_real	3.5670e+07
i0_real_error	4.6180e+05
rg_reciprocal	21.78 Å
i0_reciprocal	35670000.0000
total_estimate	0.8799
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	28.9 Å
skewness	0.203
kurtosis	-0.288
angular_range	— – 0.3650 Å⁻¹
current_alpha	0.0000
highest_alpha	7359000.0000
n_real_points	68
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.820; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.973

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	ENDOGLUCANASE I
formula_weight	44552.1 kDa
ec_number	3.2.1.4
mutation	YES
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	3726

QKPGETKEVHPQLTTFRCTKRGGCKPATNFIVLDSLSHPIHRAEGLGPGGCGDWGNPPPKDVCPDVESCAKNCIMEGIPDYSQYGVTTNGTSLRLQHILPDGRVPSPRVYLLDKTKRRYEMLHLTGFEFTFDVDATKLPCGMNSALYLSEMHPTGAKSKYNPGGAYYGTGYCDAQCFVTPFINGLGNIEGKGSCCNAMDIWEANSRASHVAPHTCNKKGLYLCEGEECAFEGVCDKNGCGWNNYRVNVTDYYGRGEEFKVNTLKPFTVVTQFLANRRGKLEKIHRFYVQDGKVIESFYTNKEGVPYTNMIDDEFCEATGSRKYMELGATQGMGEALTRGMVLAMSIWWDQGGNMEWLDHGEAGPCAKGEGAPSNIVQVEPFPEVTYTNLRWGEIGSTYQELQ

Source

organism	HUMICOLA INSOLENS
ncbi_taxonomy_id	34413
host_organism	ASPERGILLUS ORYZAE

Entity 2 non-polymer

description	2-acetamido-2-deoxy-beta-D-glucopyranose
formula_weight	221.2 kDa
src_method	man
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	595

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6. Citations (2)

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length_a	49.969 Å
length_b	74.284 Å
length_c	60.146 Å
angle_alpha	90.00 °
angle_beta	103.82 °
angle_gamma	90.00 °
z_pdb	2

space_group	P 1 21 1
int_tables_number	4
crystal_system	—

resolution	1.750 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1580
r_free	0.2000

fit	/app/data/saxs_computation/dy/1dym/1dym.fit
pddf	/app/data/pr_computation/dy/1dym/1dym.pr.out
profile	/app/data/saxs_computation/dy/1dym/1dym.dat

Humicola insolens Endocellulase Cel7B (EG 1) E197A Mutant

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction