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1dzw

L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant F131A

Method: X-RAY DIFFRACTION D_max: 60.9 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1dzw
deposition_date	2000-03-07
title	L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant F131A
keywords	;LYASE (ALDEHYDE), ALDOLASE (CLASS II), BACTERIAL L-FUCOSE METABOLISM, CLEAVAGE OF L-FUCULOSE-1-PHOSPHATE TO DIHYDROXYACETONEPHOSPHATE AND L-LACTALDEHYDE, MUTANT STRUCTURE ;; LYASE (ALDEHYDE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	17.91 Å
rg_electron	17.16 Å
i0	9973050.00
molecular_weight	22923.0 kDa
excluded_volume	28526 Å³
envelope_volume	33067 Å³
shell_volume	16379 Å³
envelope_diameter	62.9 Å
shell_rg	23.05 Å
envelope_rg	17.61 Å
shape_rg	17.17 Å
total_rg	18.06 Å
total_atoms	1602
n_residues	206
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	60.9 Å
rg_real	17.88 Å
rg_real_error	0.45 Å
i0_real	9.9730e+06
i0_real_error	1.2740e+05
rg_reciprocal	17.88 Å
i0_reciprocal	9973000.0000
total_estimate	0.6047
solution_quality	REASONABLE a REASONABLE solution
n_peaks	2
r_peak_primary	21.4 Å
skewness	0.382
kurtosis	-0.107
angular_range	— – 0.4450 Å⁻¹
current_alpha	0.0000
highest_alpha	3304000.0000
n_real_points	75
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.729; Stabil: 0.997; Sysdev: 0.227; Positv: 1.000; Valcen: 0.997; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	L-fuculose phosphate aldolase
formula_weight	23729.2 kDa
ec_number	4.1.2.17
mutation	YES
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	P
atom_count	1702

MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTGIPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRSIPAIHYMIAAAGGNSIPCAPYATAGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	2

Entity 3 non-polymer

description	BETA-MERCAPTOETHANOL
formula_weight	78.1 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	1

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	100

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6. Citations (3)

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length_a	93.900 Å
length_b	93.900 Å
length_c	43.300 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 4 21 2
int_tables_number	90
crystal_system	—

resolution	2.170 Å
resolution_low	10.000 Å
r_factor	—
r_work	0.1520
r_free	0.2180

fit	/app/data/saxs_computation/dz/1dzw/1dzw.fit
pddf	/app/data/pr_computation/dz/1dzw/1dzw.pr.out
profile	/app/data/saxs_computation/dz/1dzw/1dzw.dat