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1e3p

tungstate derivative of Streptomyces antibioticus PNPase/GPSI enzyme

Method: X-RAY DIFFRACTION D_max: 96.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e3p
deposition_date	2000-06-20
title	tungstate derivative of Streptomyces antibioticus PNPase/GPSI enzyme
keywords	POLYRIBONUCLEOTIDE TRANSFERASE, ATP-GTP DIPHOSPHOTRANSFERASE RNA PROCESSING, RNA DEGRADATION; POLYRIBONUCLEOTIDE TRANSFERASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	27.49 Å
rg_electron	26.51 Å
i0	82916900.00
molecular_weight	68333.0 kDa
excluded_volume	84321 Å³
envelope_volume	109620 Å³
shell_volume	34152 Å³
envelope_diameter	101.7 Å
shell_rg	33.90 Å
envelope_rg	27.29 Å
shape_rg	26.59 Å
total_rg	27.01 Å
total_atoms	4775
n_residues	645
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	96.6 Å
rg_real	27.46 Å
rg_real_error	0.70 Å
i0_real	8.2920e+07
i0_real_error	1.1880e+06
rg_reciprocal	27.47 Å
i0_reciprocal	82920000.0000
total_estimate	0.8599
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	33.3 Å
skewness	0.384
kurtosis	-0.106
angular_range	— – 0.2900 Å⁻¹
current_alpha	0.0000
highest_alpha	14890000.0000
n_real_points	59
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.728; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.996

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	Polyribonucleotide nucleotidyltransferase
formula_weight	81225.4 kDa
ec_number	2.7.7.8
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5080

ASMTGGQQMGRGSGSEFMENETHYAEAVIDNGAFGTRTIRFETGRLARQAAGSAVAYLDDDTMVLSATTASKNPKDQLDFFPLTVDVEERMYAAGKIPGSFFRREGRPSEDAILTCRLIDRPLRPSFKKGLRNEIQVVATIMALNPDHLYDVVAINAASASTQLAGLPFSGPIGGVRVALIRGQWVAFPTHTELEDAVFDMVVAGRVLEDGDVAIMMVEAEATEKTIQLVKDGAEAPTEEVVAAGLDAAKPFIKVLCKAQADLAAKAAKPTGEFPVFLDYQDDVLEALSAAVRPELSAALTIAGKQDREAELDRVKALAAEKLLPEFEGREKEISAAYRALTKSLVRERVIAEKKRIDGRGVTDIRTLAAEVEAIPRVHGSALFERGETQILGVTTLNMLRMEQQLDTLSPVTRKRYMHNYNFPPYSVGETGRVGSPKRREIGHGALAERAIVPVLPTREEFPYAIRQVSEALGSNGSTSMGSVCASTMSLLNAGVPLKAPVAGIAMGLISQEINGETHYVALTDILGAEDAFGDMDFKVAGTKEFVTALQLDTKLDGIPASVLAAALKQARDARLHILDVMMEAIDTPDEMSPNAPRIITVKIPVDKIGEVIGPKRQMINQIQEDTGAEITIEDDGTIYIGAADGPAAEAARATINGIANPTSPEVGERILGSVVKTTTFGAFVSLLPGKDGLLHISQIRKLAGGKRVENVEDVLGVGQKVQVEIAEIDSRGKLSLIPVIEGEEAASDEKKDDAEQ

Source

organism	Streptomyces antibioticus
ncbi_taxonomy_id	1890
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	10

Entity 3 non-polymer

description	TUNGSTATE(VI)ION
formula_weight	247.8 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	305

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6. Citations (1)

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length_a	130.830 Å
length_b	130.830 Å
length_c	328.732 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	18

space_group	H 3 2
int_tables_number	155
crystal_system	—

resolution	2.500 Å
resolution_low	19.840 Å
r_factor	0.2130
r_work	0.2130
r_free	0.2470

fit	/app/data/saxs_computation/e3/1e3p/1e3p.fit
pddf	/app/data/pr_computation/e3/1e3p/1e3p.pr.out
profile	/app/data/saxs_computation/e3/1e3p/1e3p.dat

tungstate derivative of Streptomyces antibioticus PNPase/GPSI enzyme

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction